Missing Iron-Oxidizing Acidophiles Highly Sensitive to Organic Compounds

Ueoka, Nagayoshi; Kouzuma, Atsushi; Watanabe, Kazuya

doi:10.1264/jsme2.me16086

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…E6-1, 3-8 HM769771 (Figures 3 and 4, tailings and TIF) and Acidithiobacillus sp. NU-1_LC115032 ( Figures 3 and 4, bacterial enrichment) are phylogenetically similar to A. ferrooxidans ATCC19859 [38,39]. The detection of these acidophilic, iron-oxidizing bacteria is consistent with microbial communities from acid mine drainage environments [2,3,10,[17][18][19]21,[40][41][42][43].…”

Section: Interpreting Biogeochemical Processes Contributing To Tif Dementioning

confidence: 55%

Terraced Iron Formations: Biogeochemical Processes Contributing to Microbial Biomineralization and Microfossil Preservation

et al. 2018

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Terraced iron formations (TIFs) are laminated structures that cover square meter-size areas on the surface of weathered bench faces and tailings piles at the Mount Morgan mine, which is a non-operational open pit mine located in Queensland, Australia. Sampled TIFs were analyzed using molecular and microanalytical techniques to assess the bacterial communities that likely contributed to the development of these structures. The bacterial community from the TIFs was more diverse compared to the tailings on which the TIFs had formed. The detection of both chemolithotrophic iron-oxidizing bacteria, i.e., Acidithiobacillus ferrooxidans and Mariprofundus ferrooxydans, and iron-reducing bacteria, i.e., Acidobacterium capsulatum, suggests that iron oxidation/reduction are continuous processes occurring within the TIFs. Acidophilic, iron-oxidizing bacteria were enriched from the TIFs. High-resolution electron microscopy was used to characterize iron biomineralization, i.e., the association of cells with iron oxyhydroxide mineral precipitates, which served as an analog for identifying the structural microfossils of individual cells as well as biofilms within iron oxyhydroxide laminations—i.e., alternating layers containing schwertmannite (Fe16O16(OH)12(SO4)2) and goethite (FeO(OH)). Kinetic modeling estimated that it would take between 0.25–2.28 years to form approximately one gram of schwertmannite as a lamination over a one-m2 surface, thereby contributing to TIF development. This length of time could correspond with seasonable rainfall or greater than average annual rainfall. In either case, the presence of water is critical for sustaining microbial activity, and subsequently iron oxyhydroxide mineral precipitation. The TIFs from the Mount Morgan mine also contain laminations of gypsum (CaSO·2H2O) alternating with iron oxyhydroxide laminations. These gypsum laminations likely represented drier periods of the year, in which millimeter-size gypsum crystals presumably precipitated as water gradually evaporated. Interestingly, gypsum acted as a substrate for the attachment of cells and the growth of biofilms that eventually became mineralized within schwertmannite and goethite. The dissolution and reprecipitation of gypsum suggest that microenvironments with circumneutral pH conditions could exist within TIFs, thereby supporting iron oxidation under circumneutral pH conditions. In conclusion, this study highlights the relationship between microbes for the development of TIFs and also provides interpretations of biogeochemical processes contributing to the preservation of bacterial cells and entire biofilms under acidic conditions.

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Section: Interpreting Biogeochemical Processes Contributing To Tif Dementioning

confidence: 55%

Terraced Iron Formations: Biogeochemical Processes Contributing to Microbial Biomineralization and Microfossil Preservation

et al. 2018

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“…In another study, it was observed that the presence of lead, plastics, and other nonmetallic portions was found to be responsible for the reduced bacterial growth. 37 In a bioleaching process, the volume of inoculum is also considered to be a critical factor which directly influences the rate of bioleaching, as the number of active bacteria present in the medium increases with a rise in the volume of the inoculum. 31 Figure 8A depicts that there was an increase in the initial frequency of Cu extraction from CPCBs with an increase in the volume of inoculum from 5% to 25%.…”

Section: Resultsmentioning

confidence: 99%

Neural network prediction of bioleaching of metals from waste computer printed circuit boards using Levenberg‐Marquardt algorithm

Annamalai

Gurumurthy

2020

Computational Intelligence

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The applicability of artificial neural network (ANN) to predict the bioleaching of metals using from computer printed circuit boards (CPCB) and the influence of process parameters were studied. The influence of process parameters initial pH (1.6-2.4), pulp density (2%-13%), and the initial volume of Inoculum (5%-25%) were investigated on the rate of bioleaching of metals from CPCB. Network inputs were fed as initial pH, pulp density, and inoculum volume and with the extraction of Cu, Ag, and Au as output. The ANN was developed using the Levenberg-Marquardt algorithm and trained for modeling and prediction. The most fitting architectures for Cu, Ag, and Au were [

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“…The doubling time for each condition were compared (Ccorahua-Santo et al 2017) using the one-way ANOVA test followed by Tukey's test using GraphPad Prism version 6.0.0 for Windows (www.graphpad.com). The differences were considered to be significant at P < 0.05 (Ueoka et al 2016).…”

Section: Effect Of Temperature On Doubling Time In a Ferrooxidans Atc...mentioning

confidence: 99%

Ausencia de síntesis de trehalosa en Acidithiobacillus ferrooxidans ATCC 23270 y Acidithiobacillus ferrivorans CF27 a baja temperatura

2023

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La trehalosa es un tipo de carbohidrato, que en procariotas protege contra diferentes tipos de estrés y también se utiliza como fuente de almacenamiento de carbono. Hay cuatro formas diferentes de sintetizar trehalosa en Acidithiobacillus ferrivorans y dos en Acidithiobacillus ferrooxidans, pero su propósito sigue siendo desconocido. Este estudio tuvo como objetivo medir la producción de trehalosa en diferentes condiciones mediante su cuantificación en tres medios de cultivo a dos temperaturas diferentes. También se evaluó la cinética de crecimiento de ambas especies y se analizó la concentración de trehalosa durante la fase estacionaria temprana mediante un método enzimático. Los resultados mostraron que el medio 9K modificado con hierro ferroso a 28 °C tuvo la mayor producción de trehalosa, con A. ferrivorans CF27 con una mayor producción de 0.34 µmol/mg de proteína en comparación con A. ferrooxidans ATCC 23270 a 0.31 µmol/mg de proteína. Al utilizar CuS, la producción de trehalosa fue menor, con 0.02 y 0.03 µmol/mg de proteína para A. ferrivorans CF27 y A. ferrooxidans ATCC 23270, respectivamente, mientras que en presencia de zinc no se detectó trehalosa. A 15°C, el método enzimático no detectó trehalosa en los tres medios de cultivo, lo que indicaria que este carbohidrato no protege contra las bajas temperaturas en ninguna de las especies.

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Missing Iron-Oxidizing Acidophiles Highly Sensitive to Organic Compounds

Cited by 9 publications

References 30 publications

Terraced Iron Formations: Biogeochemical Processes Contributing to Microbial Biomineralization and Microfossil Preservation

Terraced Iron Formations: Biogeochemical Processes Contributing to Microbial Biomineralization and Microfossil Preservation

Neural network prediction of bioleaching of metals from waste computer printed circuit boards using Levenberg‐Marquardt algorithm

Ausencia de síntesis de trehalosa en Acidithiobacillus ferrooxidans ATCC 23270 y Acidithiobacillus ferrivorans CF27 a baja temperatura

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