Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis

Mangeon, Amanda; Bell, Elizabeth M.; Lin, Wan-ching; Jablonska, Barbara; Springer, Patricia S.

doi:10.1093/jxb/erq259

Cited by 47 publications

(46 citation statements)

References 71 publications

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“…While knockout mutations of most LBD genes show no obvious phenotypes, their overexpression in many cases results in leaf phenotypes like lobed and curled leaves, along with dwarfing and degrees of infertility (Shuai et al, 2002;Nakazawa et al, 2003;Naito et al, 2007;Mangeon et al, 2011). Similarly, we observed no obvious morphological changes in leaf or root morphology in either lbd20 mutant, however both lines exhibited increased resistance to F. oxysporum accompanied by increased expression of a subset of JA-regulated defense genes.…”

Section: Discussionmentioning

confidence: 53%

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

et al. 2012

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The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family encodes plant-specific transcriptional regulators functioning in organ development. In a screen of Arabidopsis (Arabidopsis thaliana) sequence-indexed transferred DNA insertion mutants, we found disruption of the LOB DOMAIN-CONTAINING PROTEIN20 (LBD20) gene led to increased resistance to the root-infecting vascular wilt pathogen Fusarium oxysporum. In wild-type plants, LBD20 transcripts were barely detectable in leaves but abundant in roots, where they were further induced after F. oxysporum inoculation or methyl jasmonate treatment. Induction of LBD20 expression in roots was abolished in coronatine insensitive1 (coi1) and myc2 (allelic to jasmonate insensitive1) mutants, suggesting LBD20 may function in jasmonate (JA) signaling. Consistent with this, expression of the JA-regulated THIONIN2.1 (Thi2.1) and VEGETATIVE STORAGE PROTEIN2 (VSP2) genes were up-regulated in shoots of lbd20 following treatment of roots with F. oxysporum or methyl jasmonate. However, PLANT DEFENSIN1.2 expression was unaltered, indicating a repressor role for LBD20 in a branch of the JA-signaling pathway. Plants overexpressing LBD20 (LBD20-OX) had reduced Thi2.1 and VSP2 expression. There was a significant correlation between increased LBD20 expression in the LBD20-OX lines with both Thi2.1 and VSP2 repression, and reduced survival following F. oxysporum infection. Chlorosis resulting from application of F. oxysporum culture filtrate was also reduced in lbd20 leaves relative to the wild type. Taken together, LBD20 is a F. oxysporum susceptibility gene that appears to regulate components of JA signaling downstream of COI1 and MYC2 that are required for full elicitation of F. oxysporum-and JA-dependent responses. To our knowledge, this is the first demonstration of a role for a LBD gene family member in either biotic stress or JA signaling.

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Section: Discussionmentioning

confidence: 53%

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

et al. 2012

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“…LBD genes are suggested to be involved in developmental processes, including leaf polarity establishment (Lin et al, 2003;Xu et al, 2003) and lateral root formation (Inukai et al, 2005;Liu et al, 2005;Okushima et al, 2007). Recent results also suggest some LBDs be involved in auxin signaling (Lin et al, 2003;Inukai et al, 2005;Taramino et al, 2007;Mangeon et al, 2011). The USP protein family has been identified and studied in bacteria, but its biochemical role is not yet fully understood.…”

Section: Resultsmentioning

confidence: 99%

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

et al. 2012

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Reactive oxygen species (ROS) play an important role as triggers of gene expression during biotic and abiotic stresses, among which is low oxygen (O 2 ). Previous studies have shown that ROS regulation under low O 2 is driven by a RHO-like GTPase that allows tight control of hydrogen peroxide (H 2 O 2 ) production. H 2 O 2 is thought to regulate the expression of heat shock proteins, in a mechanism that is common to both O 2 deprivation and to heat stress. In this work, we used publicly available Arabidopsis (Arabidopsis thaliana) microarray datasets related to ROS and O 2 deprivation to define transcriptome convergence pattern. Our results show that although Arabidopsis response to anoxic and hypoxic treatments share a common core of genes related to the anaerobic metabolism, they differ in terms of ROS-related gene response. We propose that H 2 O 2 production under O 2 deprivation is a trait present in a very early phase of anoxia, and that ROS are needed for the regulation of a set of genes belonging to the heat shock protein and ROS-mediated groups. This mechanism, likely not regulated via the N-end rule pathway for O 2 sensing, is probably mediated by a NADPH oxidase and it is involved in plant tolerance to the stress.

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“…Several transcription factors such as the zinc-finger protein, CCCH-type family protein, auxin-responsive AUX IAA family member protein, homeobox, HSP, and MADS-BOX were upregulated in HKI 1105 (tolerant) as well as in Arabidopsis [50], whereas homeobox genes were down-regulated in V 372 (susceptible). The gene coding for LOB-domain proteins, which are plant-specific transcription factors involved in forming lateral roots [51], and responding to auxin [52], was significantly expressed in HKI 1105.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression of Transcriptomes and Their Co-Expression Pattern in Subtropical Maize (Zea mays L.) under Waterlogging Stress

et al. 2013

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Waterlogging causes extensive damage to maize crops in tropical and subtropical regions. The identification of tolerance genes and their interactions at the molecular level will be helpful to engineer tolerant genotypes. A whole-genome transcriptome assay revealed the specific role of genes in response to waterlogging stress in susceptible and tolerant genotypes. Genes involved in the synthesis of ethylene and auxin, cell wall metabolism, activation of G-proteins and formation of aerenchyma and adventitious roots, were upregulated in the tolerant genotype. Many transcription factors, particularly ERFs, MYB, HSPs, MAPK, and LOB-domain protein were involved in regulation of these traits. Genes responsible for scavenging of ROS generated under stress were expressed along with those involved in carbohydrate metabolism. The physical locations of 21 genes expressed in the tolerant genotype were found to correspond with the marker intervals of known QTLs responsible for development of adaptive traits. Among the candidate genes, most showed synteny with genes of sorghum and foxtail millet. Co-expression analysis of 528 microarray samples including 16 samples from the present study generated seven functional modules each in the two genotypes, with differing characteristics. In the tolerant genotype, stress genes were co-expressed along with peroxidase and fermentation pathway genes.

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Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis

Cited by 47 publications

References 71 publications

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

Genome-Wide Expression of Transcriptomes and Their Co-Expression Pattern in Subtropical Maize (Zea mays L.) under Waterlogging Stress

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