MisR/MisS Two-Component Regulon in
            <i>Neisseria meningitidis</i>

Tzeng, Yih-Ling; Kahler, Charlene M.; Zhang, Xinjian; Stephens, David S.

doi:10.1128/iai.01007-07

Cited by 43 publications

(75 citation statements)

References 69 publications

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“…Two independent transcriptomic analyses reported lists of genes whose expression was altered in meningococcal strains inactivated for the NMA0798 gene (Newcombe et al, 2005;Tzeng et al, 2008). Only 29 genes were identified in both studies and among them none of the REP2 genes.…”

Section: Discussionmentioning

confidence: 99%

“…Tzeng et al (2008) proposed a consensus sequence for the NMA0798 DNA binding site, i.e. (A/T)(A/T)TGTAA(A/G/ C)G. In our study, DNase I footprinting mapping of the NMA0798 binding sites in the REP2 sequence identified sequences similar to the core consensus sequence for the NMA0798 binding site previously reported (Tzeng et al, 2008). The differences in affinity between the NMA0798 promoter and the REP2 sequences for the NMA0798 protein evidenced in EMSAs could be related to differences in sequence from the consensus proposed by Tzeng et al (2008).…”

Section: Discussionmentioning

confidence: 99%

“…The translation start codon of the xseB gene is indicated in bold. The core consensus sequence for the NMA0798 binding site previously reported (Tzeng et al, 2008) is underlined.…”

Section: Discussionmentioning

confidence: 99%

“…This TCS was also shown to be necessary for the bacterium to cross an epithelial cell layer (Johnson et al, 2001), and was demonstrated to constitute a functional signal transduction system (Tzeng et al, 2006) that modulates the meningococcal virulence factor lipopolysaccharide (Tzeng et al, 2004). In addition, transcriptomic analyses revealed that inactivation of NMA0798 perturbed the expression of a large number of genes (Newcombe et al, 2005), many of which are involved in the synthesis of components of the meningococcal cell surface (Tzeng et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Experimental evidence demonstrating a regulatory function is only available for one of these four TCSs, i.e. the NMA0797 (PhoQ, MisS)/0798 (PhoP, MisR) system (Johnson et al, 2001;Newcombe et al, 2004Newcombe et al, , 2005Tzeng et al, 2004Tzeng et al, , 2006Tzeng et al, , 2008. A meningococcal knockout mutant in the NMA0798 gene was reported to display an attenuated virulence phenotype in a mouse model of infection (Newcombe et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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A two-component system is required for colonization of host cells by meningococcus

et al. 2009

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In order to adapt to changing environments, bacteria have evolved two-component systems (TCSs) that are able to sense and respond to environmental stimuli. The signal perception relies on a sensor protein whose activation allows rapid adaptation through transcriptional regulation achieved by the regulatory protein. The ability to adhere to and grow on the surface of human host cells is an absolute requirement for many pathogens, including Neisseria meningitidis, in order to colonize new hosts and to disseminate inside their host. Among the four TCSs encoded in the meningococcus genome, only the PhoQ (MisS)/PhoP (MisR) system has been shown to constitute a functional signal transduction circuit. To investigate the involvement of this TCS in the adaptation process requisite for host cell colonization, we have tested the ability to grow on host cells of a mutant inactivated for the sensor of the TCS. Our results demonstrate the involvement of the TCS in the adaptation of the meningococcus to growth on host cells. We show that the expression of the PhoQ (MisS)/PhoP (MisR) TCS is cell-contact controlled. Furthermore, this TCS controls the regulation of a group of genes, the REP2 regulon, previously shown to be cellcontact regulated and to encode functions crucial for the adaptation of the bacterium to host cell colonization. Thus, we provide evidence that one of the four TCSs existing in N. meningitidis contributes to the adaptation of the pathogen to growth on host cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The translation start codon of the xseB gene is indicated in bold. The core consensus sequence for the NMA0798 binding site previously reported (Tzeng et al, 2008) is underlined.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A two-component system is required for colonization of host cells by meningococcus

et al. 2009

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Regulation of Neisseria meningitidis cytochrome bc₁ components by NrrF, a Fur‐controlled small noncoding RNA

et al. 2017

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NrrF is a small regulatory RNA of the human pathogen Neisseria meningitidis . NrrF was previously shown to repress succinate dehydrogenase ( sdh CDAB ) under control of the ferric uptake regulator (Fur). Here, we provide evidence that cytochrome bc 1 , encoded by the polycistronic mRNA pet ABC , is a NrrF target as well. We demonstrated differential expression of cytochrome bc 1 comparing wild‐type meningococci and meningococci expressing NrrF when sufficient iron is available. Using a gfp ‐reporter system monitoring translational control and target recognition of sRNA in Escherichia coli , we show that interaction between NrrF and the 5′ untranslated region of the pet ABC mRNA results in its repression. The NrrF region essential for repression of pet ABC was identified by site‐directed mutagenesis and is fully conserved among meningococci. Our results provide further insights into the mechanism by which Fur controls essential components of the N. meningitidis respiratory chain. Adaptation of cytochrome bc 1 complex component levels upon iron limitation is post‐transcriptionally regulated via the small regulatory RNA NrrF.

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Moonlighting Functions of Heat Shock Protein 90

Chen

Jeffery

2019

Heat Shock Proteins

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MisR/MisS Two-Component Regulon in Neisseria meningitidis

Cited by 43 publications

References 69 publications

A two-component system is required for colonization of host cells by meningococcus

A two-component system is required for colonization of host cells by meningococcus

Regulation of Neisseria meningitidis cytochrome bc₁ components by NrrF, a Fur‐controlled small noncoding RNA

Moonlighting Functions of Heat Shock Protein 90

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MisR/MisS Two-Component Regulon in Neisseria meningitidis

Cited by 43 publications

References 69 publications

A two-component system is required for colonization of host cells by meningococcus

A two-component system is required for colonization of host cells by meningococcus

Regulation of Neisseria meningitidis cytochrome bc1 components by NrrF, a Fur‐controlled small noncoding RNA

Moonlighting Functions of Heat Shock Protein 90

Contact Info

Product

Resources

About

Regulation of Neisseria meningitidis cytochrome bc₁ components by NrrF, a Fur‐controlled small noncoding RNA