MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity

Peirsman, Arne; Blondeel, Eva; Ahmed, Tasdiq; Anckaert, Jasper; Audenaert, Dominique; Boterberg, Tom; Buzás, Krisztina; Carragher, Neil O.; Castellani, Gastone; Castro, Flávia; Dangles‐Marie, Virginie; Dawson, John C.; Tullio, Pascal De; Vlieghere, Elly De; Dedeyne, Sándor; Depypere, Herman; Diósdi, Ákos; Dmitriev, Ruslan I.; Dolznig, Helmut; Fischer, Suzanne; Gespach, Christian; Goossens, Vera; Heino, Jyrki; Hendrix, An; Horváth, Péter; Kunz‐Schughart, Leoni A.; Maes, Sebastiaan; Mangodt, Christophe; Mestdagh, Pieter; Michlíková, Soňa; Oliveira, Maria José; Pampaloni, Francesco; Piccinini, Filippo; Pinheiro, Cláudio; Rahn, Jennifer J.; Robbins, Stephen M.; Siljamäki, Elina; Steigemann, Patrick; Sys, Gwen; Takayama, Shuichi; Tesei, Anna; Tulkens, Joeri; Waeyenberge, Michiel Van; Vandesompele, Jo; Wagemans, Glenn; Weindorfer, Claudia; Yigit, Nurten; Zablowsky, Nina; Zanoni, Michele; Blondeel, Phillip; Wever, Olivier De

doi:10.1038/s41592-021-01291-4

Cited by 47 publications

(46 citation statements)

References 36 publications

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“…With the progress in tissue bioengineering and biofabrication, the necessity of O 2 quantification in microaggregates and spheroid 3D tissue constructs becomes important. One of the concerns is the standardization of the multicellular spheroid model physiological parameters, which depend on the method of spheroid generation and culture conditions 8 . In addition, uncontrolled application of O 2 releasing biomaterials or O 2 perfusion in microtissues lacking functional vascularization can be toxic to cells, lead to reprogramming of their metabolism, and decreased survival in the post-transplantation period 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Okkelman

Vercruysse

Kondrashina³

et al. 2022

JoVE

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Multicellular spheroids are important tools for studying tissue and cancer physiology in 3D and are frequently used in tissue engineering as tissue assembling units for biofabrication. While the main power of the spheroid model is in mimicking physicalchemical gradients at the tissue microscale, the real physiological environment (including dynamics of metabolic activity, oxygenation, cell death, and proliferation) inside the spheroids is generally ignored. At the same time, the effects of the growth medium composition and the formation method on the resulting spheroid phenotype are well documented. Thus, characterization and standardization of spheroid phenotype are required to ensure the reproducibility and transparency of the research results. The analysis of average spheroid oxygenation and the value of O 2 gradients in three dimensions (3D) can be a simple and universal way for spheroid phenotype characterization, pointing at their metabolic activity, overall viability, and potential to recapitulate in vivo tissue microenvironment. The visualization of 3D oxygenation can be easily combined with multiparametric analysis of additional physiological parameters (such as cell death, proliferation, and cell composition) and applied for continuous oxygenation monitoring and/or end-point measurements. The loading of the O 2 probe is performed during the stage of spheroid formation and is compatible with various protocols of spheroid generation. The protocol includes a high-throughput method of spheroid generation with introduced red and nearinfrared emitting ratiometric fluorescent O 2 nanosensors and the description of multiparameter assessment of spheroid oxygenation and cell death before and after bioprinting. The experimental examples show comparative O 2 gradients analysis in homo-and hetero-cellular spheroids as well as spheroid-based bioprinted constructs.

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Section: Introductionmentioning

confidence: 99%

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Okkelman

Vercruysse

Kondrashina³

et al. 2022

JoVE

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“…However, protocols for 3D spheroid assays vary significantly between different laboratories and are often not adequately described in research publications or provide sufficient mechanistic information of compound/drug activity. An international consortium (Mi-SpheroID) has defined and recommended a minimum set of experimental parameters (cell line, spheroid formation method; media; and spheroid size) required to report spheroid research to improve interlaboratory reproducibility and data mining across multiple studies [22] . Many 3D spheroid studies only report size as the primary assay endpoint, however spheroid size provide little information on compound mechanism-of-action and can sometimes be misleading.…”

Section: Discussionmentioning

confidence: 99%

Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis

Culley¹,

Nagle²,

Dawson³

et al. 2023

SLAS Discovery

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“…This physical parameter allows for the development of superficial and inner zones with different nutrients and oxygen gradients, a structural characteristic of the tumour mass [ 138 , 139 , 140 ]. Oxygen tension and permeability are features that affect the chemo- and radio-cytotoxicity on tumour cells [ 141 , 142 , 143 ]. For these reasons, scaffold-free cultures are excellent and representative systems for in vitro drug screening assays.…”

Section: Precision Medicine In Hnsccsmentioning

confidence: 99%

Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches

Miserocchi

Spadazzi

Calpona

et al. 2022

JPM

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Head and neck cancers (HNCs) represent the sixth most widespread malignancy worldwide. Surgery, radiotherapy, chemotherapeutic and immunotherapeutic drugs represent the main clinical approaches for HNC patients. Moreover, HNCs are characterised by an elevated mutational load; however, specific genetic mutations or biomarkers have not yet been found. In this scenario, personalised medicine is showing its efficacy. To study the reliability and the effects of personalised treatments, preclinical research can take advantage of next-generation sequencing and innovative technologies that have been developed to obtain genomic and multi-omic profiles to drive personalised treatments. The crosstalk between malignant and healthy components, as well as interactions with extracellular matrices, are important features which are responsible for treatment failure. Preclinical research has constantly implemented in vitro and in vivo models to mimic the natural tumour microenvironment. Among them, 3D systems have been developed to reproduce the tumour mass architecture, such as biomimetic scaffolds and organoids. In addition, in vivo models have been changed over the last decades to overcome problems such as animal management complexity and time-consuming experiments. In this review, we will explore the new approaches aimed to improve preclinical tools to study and apply precision medicine as a therapeutic option for patients affected by HNCs.

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MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity

Cited by 47 publications

References 36 publications

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis

Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches

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