Mismatch recognition function of Arabidopsis thaliana MutSγ

Gómez, Rodrigo; Spampinato, Claudia P.

doi:10.1016/j.dnarep.2013.01.002

Cited by 20 publications

(12 citation statements)

References 40 publications

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“…MSH7 is a plant-specific MutS protein sharing high similarity with MSH6 (Adé et al, 1999). The Arabidopsis MSH2-MSH7 complex was shown to have the capacity to recognize specific base-base mismatches (Culligan and Hays, 2000;Wu et al, 2003;Gómez and Spampinato, 2013). During meiosis, MSH7 is suggested to be associated with homoeologous recombination (Dong et al, 2002;Tam et al, 2011).…”

Section: Meica1 May Act To Suppress Nahrmentioning

confidence: 99%

Meiotic Chromosome Association 1 Interacts with TOP3α and Regulates Meiotic Recombination in Rice

Wang

et al. 2017

Plant Cell

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Homologous recombination plays a central role in guaranteeing chromosome segregation during meiosis. The precise regulation of the resolution of recombination intermediates is critical for the success of meiosis. Many proteins, including the RECQ DNA helicases (Sgs1/BLM) and Topoisomerase 3α (TOP3α), have essential functions in managing recombination intermediates. However, many other factors involved in this process remain to be defined. Here, we report the isolation of meiotic chromosome association 1 (MEICA1), a novel protein participating in meiotic recombination in rice (). Loss of MEICA1 leads to nonhomologous chromosome association, the formation of massive chromosome bridges, and fragmentation. MEICA1 interacts with MSH7, suggesting its role in preventing nonallelic recombination. In addition, MEICA1 has an anticrossover activity revealed by suppressing the defects of crossover formation in compared with that in, showing the similar function with its interacted protein TOP3α. Thus, our data establish two pivotal roles for MEICA1 in meiosis: preventing aberrant meiotic recombination and regulating crossover formation.

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Section: Meica1 May Act To Suppress Nahrmentioning

confidence: 99%

Meiotic Chromosome Association 1 Interacts with TOP3α and Regulates Meiotic Recombination in Rice

Wang

et al. 2017

Plant Cell

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“…a AGI Arabidopsis genome initiative b AGI locus code for components involved in DNA metabolic pathways is shown in Table 2 the role of a given plant MMR protein came from in vivo studies. Using a yeast system, we have found that AtMutSc is able to specifically recognize G/T, A/C, T/C, G/A, T/T, or A/A mismatches and/or neighboring sequences containing T [118] and that the expression of AtMutLa affected the repair of IDLs in microsatellite sequences [119]. The functionality of plant MutS and MutL homologs was also assessed using A. thaliana (reviewed in [114]), tomato [120], rice [121] and Nicotiana [122] mutant plants.…”

Section: Rmi1 At5g63540mentioning

confidence: 99%

Protecting DNA from errors and damage: an overview of DNA repair mechanisms in plants compared to mammals

Spampinato

2016

Cell. Mol. Life Sci.

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The genome integrity of all organisms is constantly threatened by replication errors and DNA damage arising from endogenous and exogenous sources. Such base pair anomalies must be accurately repaired to prevent mutagenesis and/or lethality. Thus, it is not surprising that cells have evolved multiple and partially overlapping DNA repair pathways to correct specific types of DNA errors and lesions. Great progress in unraveling these repair mechanisms at the molecular level has been made by several talented researchers, among them Tomas Lindahl, Aziz Sancar, and Paul Modrich, all three Nobel laureates in Chemistry for 2015. Much of this knowledge comes from studies performed in bacteria, yeast, and mammals and has impacted research in plant systems. Two plant features should be mentioned. Plants differ from higher eukaryotes in that they lack a reserve germline and cannot avoid environmental stresses. Therefore, plants have evolved different strategies to sustain genome fidelity through generations and continuous exposure to genotoxic stresses. These strategies include the presence of unique or multiple paralogous genes with partially overlapping DNA repair activities. Yet, in spite (or because) of these differences, plants, especially Arabidopsis thaliana, can be used as a model organism for functional studies. Some advantages of this model system are worth mentioning: short life cycle, availability of both homozygous and heterozygous lines for many genes, plant transformation techniques, tissue culture methods and reporter systems for gene expression and function studies. Here, I provide a current understanding of DNA repair genes in plants, with a special focus on A. thaliana. It is expected that this review will be a valuable resource for future functional studies in the DNA repair field, both in plants and animals.

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“…Among them, MSH2 and MLH1 are the key monomers that form heterodimers with other MMR proteins, such as MSH2–MSH6 (MutS α ), MSH2-MSH3 (MutS β ), MSH2–MSH7 (MutS γ ), MLH1-PMS1 (MutL α , for humans, MLH1-PMS2), and MLH1–MLH3 (MutL γ ) [9–13]. MutS α is mainly required to correct single base mispairs and short insertion/deletion loops, whereas MutS β is predominantly involved in the removal of large insertion/deletion loops (2–12 nucleotides), and plant specific MutS γ preferentially recognizes certain base-base mismatches [11, 14]. Thus mutation or disruption of plant MutS and MutL genes may affect DNA mismatch repair, resulting in mutations in both morphology and DNA.…”

Section: Introductionmentioning

confidence: 99%

Specific-Locus Amplified Fragment Sequencing Reveals Spontaneous Single-Nucleotide Mutations in Rice OsMsh6 Mutants

Hairui

Zhu

2017

BioMed Research International

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Genomic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. We investigated mutations arising spontaneously in rice OsMsh6 mutants by specific-locus amplified fragment sequencing. Totally 994 single-nucleotide mutations were identified in three mutants and on average the mutation density is about 1/136.72 Kb per mutant line. These mutations were relatively randomly distributed in genome and might be accumulated in generation-dependent manner. All possible base transitions and base transversions could be seen and the ratio of transitions to transversions was about 3.12. We also observed the nearest-neighbor bias around the mutated base. Our data suggests that OsMsh6 (LOC_Os09g24220) is important in ensuring genome stability by recognizing mismatches that arise spontaneously and provides useful information for investigating the function of the OsMsh6 gene in DNA repair and exploiting MMR mutants in rice induced mutation breeding.

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Mismatch recognition function of Arabidopsis thaliana MutSγ

Cited by 20 publications

References 40 publications

Meiotic Chromosome Association 1 Interacts with TOP3α and Regulates Meiotic Recombination in Rice

Meiotic Chromosome Association 1 Interacts with TOP3α and Regulates Meiotic Recombination in Rice

Protecting DNA from errors and damage: an overview of DNA repair mechanisms in plants compared to mammals

Specific-Locus Amplified Fragment Sequencing Reveals Spontaneous Single-Nucleotide Mutations in Rice OsMsh6 Mutants

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