2014
DOI: 10.1016/j.molcel.2014.01.018
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Mislocalization of the Centromeric Histone Variant CenH3/CENP-A in Human Cells Depends on the Chaperone DAXX

Abstract: Centromeres are essential for ensuring proper chromosome segregation in eukaryotes. Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals. Its overexpression in aggressive cancers raises questions concerning its effect on chromatin dynamics and contribution to tumorigenesis. We find that CenH3 overexpression in human cells leads to ectopic enrichment at sites of active histone turnover involving a heterotypic tetramer containing CenH3-H4 with H3.3… Show more

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Cited by 221 publications
(350 citation statements)
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“…The sensitivity of the uncross-linked CCAN to MNase digestion can account in part for the differences in DNA protection that led to conflicting conclusions about the structure of the CENP-A nucleosome. However, by following N-ChIP with salt fractionation, we now show that CENP-A particles observed using low-salt conditions (Lacoste et al 2014;Nechemia-Arbely et al 2017) comprise only a minor fraction of the total CENP-A genome-wide. In contrast, the major N-ChIP salt fraction consists of particles that protect much larger DNA fragments, consistent with the presence of an intact CCAN complex.…”
Section: Divergent α Satellites Retain Some Competence For Cenp-a Assmentioning
confidence: 78%
See 1 more Smart Citation
“…The sensitivity of the uncross-linked CCAN to MNase digestion can account in part for the differences in DNA protection that led to conflicting conclusions about the structure of the CENP-A nucleosome. However, by following N-ChIP with salt fractionation, we now show that CENP-A particles observed using low-salt conditions (Lacoste et al 2014;Nechemia-Arbely et al 2017) comprise only a minor fraction of the total CENP-A genome-wide. In contrast, the major N-ChIP salt fraction consists of particles that protect much larger DNA fragments, consistent with the presence of an intact CCAN complex.…”
Section: Divergent α Satellites Retain Some Competence For Cenp-a Assmentioning
confidence: 78%
“…We wondered whether the relative lack of large fragments obtained using N-ChIP in other published studies (Lacoste et al 2014;Henikoff et al 2015;Nechemia-Arbely et al 2017) might have resulted from the failure to solubilize most centromeric chromatin under low-salt conditions. To address this possibility, we prepared Illumina sequencing libraries from N-ChIP salt fractions and subjected them to paired-end sequencing.…”
Section: Ccan Complexes Extracted With High Salt Are Heterogeneous Inmentioning
confidence: 99%
“…Ordinarily, CENH3 is enriched at the positions of the centromeres but can also be present to some degree elsewhere on the chromosome (40). However, the incorporation into arms is a regular occurrence upon overexpression of CENH3 (41,42), which can produce ectopic centromeres, suggesting that a continuum of frequency of de novo centromere formation might exist. Under normal conditions, one possibility might be that any de novo centromere formation is blocked in the arms.…”
Section: Discussionmentioning
confidence: 99%
“…In fact, histone chaperones have emerged as key players in the transient disorganization of chromatin required in the DNA repair process. Thus, the histone chaperones aprataxin-PNK-like factor (APLF) (6), antisilencing function 1 (Asf1) (9), chromatin assembly factor 1 (CAF-1) (10), death domain-associated protein 6 (DAXX) (11), facilitating chromatin transcription (FACT) (12), histone regulator A (HIRA) (13), nucleolin (14), p400 (15), and nucleosome assembly protein 1-like 1 (NAP1L1) and 1-like 4 (NAP1L4) (7) are recruited to damaged chromatin, thereby promoting histone dynamics in response to DNA damage. Recently, SET/TAF-1β was found to be recruited to DNA breaks to modulate the DNA damage response through the retention of certain chromatin proteins, resulting in chromatin compaction (16).…”
mentioning
confidence: 99%