Misidentification of<i>Candida guilliermondii</i>as<i>C. famata</i>among Strains Isolated from Blood Cultures by the VITEK 2 System

Kim, Si Hyun; Shin, Jeong Hwan; Mok, Jeongha; Kim, Shine Young; Song, Sae Am; Kim, Hye Ran; Kook, Joong-Ki; Chang, Young-Hyo; Bae, Il Kwon; Lee, Kwangha

doi:10.1155/2014/250408

Cited by 25 publications

(20 citation statements)

References 28 publications

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“…The Vitek-2 Compact system performed poorly in the identification of the 164 C. guilliermondii isolates, with only 15.2% of isolates correctly identified to the species level, which is similar to the results of previous studies (16,17). Furthermore, there was also a low discrimination rate of 74.4% (122 isolates) between C. famata and C. guilliermondii, and 10.4% (17 isolates) was misidentified as C. famata.…”

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confidence: 82%

Identification and Antifungal Susceptibility Profile of Candida guilliermondii and Candida fermentati from a Multicenter Study in China

et al. 2016

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dWith molecular sequencing as a gold standard, the Vitek MS, Bruker Biotyper MS, and Vitek-2 Compact systems correctly identified 92.7%, 97.0%, and 15.2% of 164 Candida guillermondii isolates, respectively, and none of 8 C. fermentati isolates. All of the isolates showed high susceptibility to echinocandins, but some C. guilliermondii isolates showed low azole susceptibility. Invasive candidiasis (IC) is a public health threat worldwide and is considered a major cause of infection-related morbidity and mortality (1, 2). The incidence of candidemia due to Candida guilliermondii is low, ranging from 1% to 3%, depending on the geographic region (3, 4). However, the few available reports on C. guilliermondii infections indicate that these organisms are associated with poor clinical outcomes, which warrants further investigation (5-9).C. guilliermondii complex is a genetically heterogeneous group of phenotypically indistinguishable yeast species, including C. guilliermondii, C. fermentati, C. carpophila, and C. xestobii. Accurate and timely identification of C. guilliermondii complex isolates to the species level, including the associated antifungal susceptibility profiles, is essential for guiding clinical decisions (8). However, there is limited information on the performance of phenotypic methods like the Vitek-2 Compact (bioMérieux, France), and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems in the identification of species within the C. guilliermondii complex in China.C. guilliermondii complex isolates (n ϭ 172) collected from 40 hospitals in 18 provinces of China, under the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program (2010 to 2014), were studied. We evaluated the performance of the Vitek-2 Compact (bioMérieux, France) and two MALDI-TOF MS systems, including the Vitek MS system (IVD Knowledgebase version 2.0; bioMérieux) and the Bruker Autoflex Speed TOF/TOF MS system (with Biotyper version 3.1 software; Bruker Daltonics, USA) in the identification of C. guilliermondii complex isolates. Sequencing of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region was used as the gold standard (10).In vitro susceptibilities of the isolates to nine antifungal drugs were determined by the Sensititre YeastOne YO10 (Thermo Scientific, USA) system as per the manufacturer's instructions. MIC values were interpreted according to the CLSI document for echinocandins (11) and epidemiological cutoff values (ECVs) for the other agents (12). This study was approved by the relevant Human Research Ethics Committee (S-263).Isolates were derived from 172 patients (117 males and 55 females), with an average age of 54 Ϯ 23.1 years. Detailed information on the origin of the isolates (location and specimen type) is available in Table 1. The majority of the isolates were from surgical department (36.6%) and intensive care unit (ICU) (32.6%); patients admitted in these departments are more likely to have serious underlying disease or cancer or to be immunocom...

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confidence: 82%

Identification and Antifungal Susceptibility Profile of Candida guilliermondii and Candida fermentati from a Multicenter Study in China

et al. 2016

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“…Though the VITEK 2 system is popularly used in clinical microbiology laboratories, its performance is not optimal ( Kim et al, 2014 ; Karabıçak et al, 2015 ; Posteraro et al, 2015 ). Candida auris , an emerging multidrug resistant pathogen causing healthcare-associated infections ( Schelenz et al, 2016 ), was misidentified as C. haemulonii by the VITEK 2 system ( Kathuria et al, 2015 ).…”

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confidence: 99%

Oligonucleotide Array and VITEK Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Species Identification of Blood Yeast Isolates

Chang

Chen

et al. 2018

Front. Microbiol.

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We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

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“…C. guilliermondii is an uncommon species of Candida that is rarely the cause of peritonitis . Because this species is difficult to differentiate from the other Candida species by phenotypic features with the traditional identification system, misidentification of the C. guilliermondii species often occurs . Furthermore, it has lower susceptibility to antifungal agents .…”

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confidence: 99%

“…A 53-year-old man undergoing continuous ambulatory peritoneal dialysis (CAPD) presented with abdominal pain and cloudy dialysate. The white blood cell (WBC) count of dialysate effluent was 495/mm 3 . Serum C-reactive protein (CRP) level was 6.7 mg/dL.…”

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confidence: 99%

“…2 Because this species is difficult to differentiate from the other Candida species by phenotypic features with the traditional identification system, misidentification of the C. guilliermondii species often occurs. 3 Furthermore, it has lower susceptibility to antifungal agents. 4 16S rRNA gene sequencing is a useful method for rapid and accurate identification of pathogens in fungal peritonitis as well as bacterial peritonitis.…”

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Candida guilliermondii continuous ambulatory peritoneal dialysis peritonitis confirmed by 16s rRNA sequencing

et al. 2016

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Misidentification ofCandida guilliermondiiasC. famataamong Strains Isolated from Blood Cultures by the VITEK 2 System

Cited by 25 publications

References 28 publications

Identification and Antifungal Susceptibility Profile of Candida guilliermondii and Candida fermentati from a Multicenter Study in China

Identification and Antifungal Susceptibility Profile of Candida guilliermondii and Candida fermentati from a Multicenter Study in China

Oligonucleotide Array and VITEK Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Species Identification of Blood Yeast Isolates

Candida guilliermondii continuous ambulatory peritoneal dialysis peritonitis confirmed by 16s rRNA sequencing

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