Misidentification of Brucella melitensis as Ochrobactrum anthropi by API 20NE

Elsaghir, Ashraf A.F.; James, Edward

doi:10.1099/jmm.0.05153-0

Cited by 67 publications

(58 citation statements)

References 7 publications

(6 reference statements)

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“…Brucella and Ochrobactrum are phenotypically and genetically closely related micro-organisms that may cause diseases with similar clinical presentation (Elsaghir & James, 2003;Vaidya et al, 2006). Although Brucella and Ochrobactrum can be differentiated phenotypically by means of their growth behaviour, motility, lysis by Brucella-specific bacteriophages and agglutination with Brucella-specific antisera, misidentification of these organisms using biochemical test systems such as API 20NE has been reported (e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Although Brucella and Ochrobactrum can be differentiated phenotypically by means of their growth behaviour, motility, lysis by Brucella-specific bacteriophages and agglutination with Brucella-specific antisera, misidentification of these organisms using biochemical test systems such as API 20NE has been reported (e.g. Elsaghir & James, 2003;Hubalek et al, 2007). Currently, no PCR assay is available for the discrimination of O. anthropi, O. intermedium and Brucella spp.…”

Section: Resultsmentioning

confidence: 99%

“…These test systems often do not allow correct identification of the agent, and consequently misidentifications of Brucella spp. as O. anthropi (Elsaghir & James, 2003) or O. intermedium (Hubalek et al, 2007) have been reported. Furthermore, as O. anthropi is the only species of Ochrobactrum in the database of commercially available test systems such as API 20NE, differentiation of O. anthropi and O. intermedium is impossible (Teyssier et al, 2005;Kämpfer et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene

Scholz

Pfeffer

Witte

et al. 2008

Journal of Medical Microbiology

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Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. Consequently, difficulties in their identification and differentiation have been reported. In this study, a sensitive recA gene-based multi-primer single-target PCR (MP-ST-PCR) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. The specificity of the assay was evaluated using a representative panel of 50 O. anthropi and 16 O. intermedium strains and the type strains of all Brucella spp. Detection limits for purified DNA from O. anthropi, O. intermedium and Brucella melitensis were 100, 10 and 100 fg, respectively. Brucella DNA was also successfully detected in various clinical specimens from a human patient with culture-proven brucellosis and from a Brucella-infected sheep and its aborted fetuses. The sensitivity of the MP-ST-PCR was comparable to that of an evaluated in-house Brucella real-time PCR assay. The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene

Scholz

Pfeffer

Witte

et al. 2008

Journal of Medical Microbiology

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“…This kind of close relationship has been reported between B. melitensis and Ochrobactrum spp. and has also led to misidentification (Elsaghir and James 2003). Besides this phenotypic features O. anthropi is one of the closest Brucella relatives based on DNA, rRNA, and protein analyses (Yanagi and Yamasato 1993, Velasco et al 1998, Cloeckaert et al 1999.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

The mismatched isolation of Brucella strains from nomic hosts

Büyük¹,

Sidamonidze²,

Zhgenti³

et al. 2017

EurasianJVetSci

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ÖzAmaç: Bu çalışmada Kafkas Üniversitesine ait Brucella suş koleksiyonunun bir bölümü çeşitli yöntemler ile değerlendi-rilmiştir. Öneri: Bu çalışma belli konaklar arasında çapraz Brucella infeksiyonunun sıradışı olarak nitelendirilebileceğini fakat zamanla bunun olağan olabileceğini göstermektedir. Bu şe-kildeki çalışmalar, Brucella cinsinin patojenitesini, konak spesifitesini ve gelişimini daha iyi anlamaya yardımcı olacaktır. Gereç veAnahtar kelimeler: Brucella, saha suşlar, sğır, koyun, moleküler karakterizasyon Abstract Aim: A small part of field Brucella strain collection of Kafkas University was evaluated by different methods in this study. Materials and Methods:The strains were originated from cattle and sheep which had a history of abortion. Thirty field Brucella strains (16 were originated from sheep and 14 were from cattle) were randomly selected and included in the study. In addition of standardized phenotypical characterization, genus specific real-time PCR and species specific Bruce-Ladder PCR were conducted for molecular typing.Results: Out of 16 sheep and 14 cattle samples 15 and 13 were identified as B. melitensis and B. abortus, respectively. Interestingly one strain was characterized as B. abortus isolated from aborted sheep foetus and the other as B. melitensis from aborted cattle milk sample. Furthermore one strain from aborted sheep foetus was unable to be confirmed as Brucella spp. by genotypically (neither by real-time PCR nor Bruce-Ladder PCR) though all phenotypic features were similar to Brucella genus and kept under strict conditions for future characterization on suspicion of Ochrobactrum spp. Conclusion:These report alleges that Brucella crossinfection among certain hosts ceased to be extraordinary and gained an usual face over time. These kind of efforts would help in further understanding the evolution, host specificity and pathogenicity of the genus Brucella.

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“…However, identification of Brucella species using some commercial bacterial identification systems has not been consistent and misidentifications have occurred in the past [4]. Brucella species has been misidentified as Moraxella phenylpyruvica using the API 20NE system (bioMẻrieux, Marcy-I'Etoile, France) [5,6], Ochrobacterium anthropi by the API 20NE system [7] and RapID NF Plus system (Innovative Diagnostic Systems Inc., Atlanta, USA) [8], and as Haemophilus influenzae biotype IV and Moraxella species with use of MicroScan panels Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) [7].…”

Section: Introductionmentioning

confidence: 99%

16S rRNA gene sequence analysis of a Brucella melitensis infection misidentified as Bergeyella zoohelcum

Dash

Panigrahi

Al-Zarouni

et al. 2011

J Infect Dev Ctries

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Misidentification of Brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate from a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieux Inc., Durham, NC, USA). Because of this identification ambiguity by the two automated bacterial identification systems we performed 16S rRNA sequencing and serotyping of the isolate and confirmed it as a Brucella spp. Combining the sequence data with the Vitek 2 system data we conclude that the infection was caused by B. melitensis.

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Misidentification of Brucella melitensis as Ochrobactrum anthropi by API 20NE

Cited by 67 publications

References 7 publications

Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene

Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene

The mismatched isolation of Brucella strains from nomic hosts

16S rRNA gene sequence analysis of a Brucella melitensis infection misidentified as Bergeyella zoohelcum

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