Mise en évidence du premier cas de microsporidiose intestinale à Enterocytozoon bieneusi chez un patient VIH positif à Hanoï, Vietnam

Lejeune, Anne; Espern, Anne; Phung, Dac Cam; Nguyen, T.C.; Miégeville, M.

doi:10.1016/j.medmal.2005.06.003

Cited by 6 publications

(1 citation statement)

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“…We stored DNA extracts at −20°C until subsequent analysis. We completed amplification with Eb and Eb5 oligonucleotide primers at a final concentration of 0.5 μmol/L and detected the 180-bp product using the specific fluorescent TaqMan probe EbS2 in the LightCycler 480 II apparatus (Roche, https://www.roche.com) as described previously ( 7 , 21 ). We set the positive cutoff value of qPCR at < 39 quantitative cycles (Cq) and tested each clinical sample in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Genotyping Approach for Potential Common Source of Enterocytozoon bieneusi Infection in Hematology Unit

Désoubeaux¹,

Nourrisson²,

Moniot³

et al. 2019

Emerg. Infect. Dis.

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Microsporidiosis is a fungal infection that generally causes digestive disorders, especially in immunocompromised hosts. Over a 4-day period in January 2018, 3 patients with hematologic malignancies who were admitted to the hematology unit of a hospital in France received diagnoses of Enterocytozoon bieneusi microsporidiosis. This unusually high incidence was investigated by sequence analysis at the internal transcribed spacer rDNA locus and then by 3 microsatellites and 1 minisatellite for multilocus genotyping. The 3 isolates had many sequence similarities and belonged to a new genotype closely related to genotype C. In addition, multilocus genotyping showed high genetic distances with all the other strains collected from epidemiologically unrelated persons; none of these strains belonged to the new genotype. These data confirm the epidemiologic link among the 3 patients and support a common source of infection.

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Section: Methodsmentioning

confidence: 99%