MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and bacterial DNA promotes cross-priming and Th1 immunity

Girvan, Rebecca; Knight, Deborah; O'Loughlin, Chris; Hayman, Colin M.; Hermans, Ian F.; Webster, Gill

doi:10.1016/j.vaccine.2010.10.040

Cited by 38 publications

(69 citation statements)

References 55 publications

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“…Generally, Th1 cells secrete IgG2a antibody, and Th2 cells secrete IgG1 antibody, and the ratio of IgG2a/IgG1 directs a Th1-biased immune response. 44 In our study, OVA-PHYP elicited the highest IgG2a/IgG1 ratio, as illustrated in Figure 5B, which indicated an intensive bias toward Th1 compared with the empty PLA nanospheres or drug alone. Furthermore, the cellular immune responses by splenocytes and DCs from mice being inoculated were also investigated.…”

supporting

confidence: 58%

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Zheng

Qin

et al. 2018

IJN

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Background Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated. Purpose In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres. Methods The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses. Results The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4 + and CD8 + T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes). Conclusion It was corroborated that PHY- and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations.

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supporting

confidence: 58%

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Zheng

Qin

et al. 2018

IJN

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“…However, interest in the adjuvant potential of P. acnes-derived preparations continues. MIS416 is a microparticle immunostimulant comprising P. acnes whole-cells extracted with alcohols and guanidine to give a minimal cell wall skeleton rich in immunostimulatory crosslinked muramyl dipeptide repeats and native bacterial DNA fragments, each of which have known adjuvant activity [95]. There is also continued interest in the development of vaccines based on various P. acnes components, including a surface sialidase [96,97] and a secreted CAMP factor [98].…”

Section: Vaccinesmentioning

confidence: 99%

Propionibacterium acnes: infection beyond the skin

Perry¹,

Lambert

2011

Expert Review of Anti-infective Therapy

207

164

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Propionibacterium acnes is a Gram-positive bacterium that forms part of the normal flora of the skin, oral cavity, large intestine, the conjunctiva and the external ear canal. Although primarily recognized for its role in acne, P. acnes is an opportunistic pathogen, causing a range of postoperative and device-related infections. These include infections of the bones and joints, mouth, eye and brain. Device-related infections include those of joint prostheses, shunts and prosthetic heart valves. P. acnes may play a role in other conditions, including inflammation of the prostate leading to cancer, SAPHO (synovitis, acne, pustulosis, hyperostosis, osteitis) syndrome, sarcoidosis and sciatica. If an active role in these conditions is established there are major implications for diagnosis, treatment and protection. Genome sequencing of the organism has provided an insight into the pathogenic potential and virulence of P. acnes.

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“…One recent report favoured MDP as part of an experimental proteoliposome-based vaccine formulation against Candida species, since effective immune responses were induced in mice without toxic effects (Mašek et al, 2011). Similarly, administration of biodegradable microparticles comprising MDP with bacterial DNA from Propionibacterium acnes (MIS416) resulted in a nontoxic Th1 cellular adjuvant with desirable immunostimulatory properties in mice and rabbits (Girvan et al, 2011). We have previously demonstrated that microglia respond to TLR1/2, 3, 4 and 9 agonists by increasing their capacity to phagocytose and kill ingested E. coli strains (Ribes et al, 2009).…”

Section: Tablementioning

confidence: 99%

The nucleotide-binding oligomerization domain-containing-2 ligand muramyl dipeptide enhances phagocytosis and intracellular killing of Escherichia coli K1 by Toll-like receptor agonists in microglial cells

Ribes¹,

Adam²,

Schütze³

et al. 2012

Journal of Neuroimmunology

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MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and bacterial DNA promotes cross-priming and Th1 immunity

Cited by 38 publications

References 55 publications

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Propionibacterium acnes: infection beyond the skin

The nucleotide-binding oligomerization domain-containing-2 ligand muramyl dipeptide enhances phagocytosis and intracellular killing of Escherichia coli K1 by Toll-like receptor agonists in microglial cells

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