Mis16 and Mis18 Are Required for CENP-A Loading and Histone Deacetylation at Centromeres

Hayashi, Takeshi; Fujita, Yohta; Iwasaki, Osamu; Adachi, Yoh; Takahashi, Kohske; Yanagida, Mitsuhiro

doi:10.1016/j.cell.2004.09.002

Cited by 393 publications

(660 citation statements)

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“…Centromere localization limited to the period of chromosome segregation supports their role in spindle attachment. Taken together, the behavior of these groups was generally consistent with the subcomplex structures that have been identified from genetic interaction and biochemical purification studies (Hayashi et al, 2004;Obuse et al, 2004;Liu et al, 2005).…”

Section: Meiotic Behaviors Of Kinetochore Proteins Observed In Livingsupporting

confidence: 53%

“…Recently, it has been reported that S. pombe Moa1 functions in meiotic cohesin Rec8-mediated monopolar spindle attachment at meiosis I and that its centromere localization depends on Cnp3, a CENP-C homolog (Yokobayashi and Watanabe, 2005). In S. cerevisiae, centromere localization of meiotic cohesin Rec8 is reduced by loss of CHL4 (Marston et al, 2004), which is a homolog of S. pombe Mis15, and Mis15 requires Mis6 for its centromere localization (Hayashi et al, 2004). Mis6 is also required for loading of Cnp1, a CENP-A homolog (Takahashi et al, 2000).…”

Section: Mis6 Complex: Basic Architecture Of the Kinetochorementioning

confidence: 99%

“…Subcomplex structures of the S. pombe kinetochore, similar to that of S. cerevisiae and humans, have been reported (Hayashi et al, 2004;Obuse et al, 2004;Liu et al, 2005). The S. pombe kinetochore contains the Ndc80 complex (Ndc80, Nuf2, Spc24, and Spc25), which is highly conserved in many organisms from yeasts to humans (Nabetani et al, 2001;Wigge and Kilmartin, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Recently 13 proteins were purified as a Mis6-containing complex by biochemical purification . These proteins include Sim4 and Mis15, which have been reported to depend on the Mis6 protein for their centromere localization (Takahashi et al, 2000;Pidoux et al, 2003;Hayashi et al, 2004). Thus, it is likely that these proteins compose the Mis6 complex (also called the Sim4 complex in Liu et al, 2005), which corresponds to the S. cerevisiae COMA and Ctf19 complexes.…”

Section: Introductionmentioning

confidence: 99%

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Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

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During the transition from mitosis to meiosis, the kinetochore undergoes significant reorganization, switching from a bipolar to a monopolar orientation. To examine the centromere proteins that are involved in fundamental reorganization in meiosis, we observed the localization of 22 mitotic and 2 meiotic protein components of the kinetochore during meiosis in living cells of the fission yeast. We found that the 22 mitotic proteins can be classified into three groups: the Mis6-like group, the NMS (Ndc80-Mis12-Spc7) group, and the DASH group, based on their meiotic behavior. Mis6-like group proteins remain at the centromere throughout meiosis. NMS group proteins disappear from the centromere at the onset of meiosis and reappear at the centromere in two steps in late prophase. DASH group proteins appear shortly before metaphase of meiosis I. These observations suggest that Mis6-like group proteins constitute the structural basis of the centromere and that the NMS and DASH group proteins reassemble to establish the functional metaphase kinetochore. On the other hand, the meiosis-specific protein Moa1, which plays an important role in forming the meiotic monopolar kinetochore, is loaded onto the centromere significantly earlier than the NMS group, whereas another meiosis-specific protein, Sgo1, is loaded at times similar to the NMS group.

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Section: Meiotic Behaviors Of Kinetochore Proteins Observed In Livingsupporting

confidence: 53%

Section: Mis6 Complex: Basic Architecture Of the Kinetochorementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

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“…Consistent with this hypothesis, RNA interference (RNAi) is required for the localization of many kinetochore proteins Oegema et al, 2001;Goshima et al, 2003). However, several kinetochore proteins do not seem to require CenH3 for localization, suggesting that CenH3-independent assembly pathways also exist (Goshima et al, 2003;Hayashi et al, 2004;Regnier et al, 2005). In addition, the overexpression of human CenH3 fails to nucleate complete kinetochore assembly, despite driving CenH3 localization to euchromatin (Van Hooser et al, 2001).…”

Section: Introductionmentioning

confidence: 72%

De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant

Collins

Castillo

Tatsutani

et al. 2005

MBoC

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Kinetochores mediate chromosome attachment to the mitotic spindle to ensure accurate chromosome segregation. Budding yeast is an excellent organism for kinetochore assembly studies because it has a simple defined centromere sequence responsible for the localization of >65 proteins. In addition, yeast is the only organism where a conditional centromere is available to allow studies of de novo kinetochore assembly. Using a conditional centromere, we found that yeast kinetochore assembly is not temporally restricted and can occur in both G 1 phase and prometaphase. We performed the first investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4 and found that all proteins tested depend on Cse4 to localize. Consistent with this observation, Cse4-depleted cells had severe chromosome segregation defects. We therefore propose that yeast kinetochore assembly requires both centromeric DNA specificity and centromeric chromatin. INTRODUCTIONAccurate chromosome segregation in mitosis and meiosis is essential for the maintenance of genomic stability. Chromosomes attach to the mitotic spindle at the kinetochore, the protein complex that assembles onto centromeric DNA. Although kinetochore function is conserved, the underlying centromeric DNA is highly variable. Budding yeast contain a 125-base pair sequence-specific centromere that is sufficient for kinetochore formation (Fitzgerald-Hayes et al., 1982). In contrast, centromeres in multicellular eukaryotes are composed of megabases of highly repetitive DNA that lack sequence specificity (for review, see Sullivan et al., 2001). In these organisms, kinetochore assembly seems to be propagated by unidentified epigenetic component(s) (Karpen and Allshire, 1997;Sullivan et al., 2001).The best-characterized kinetochore is in budding yeast where Ͼ65 components have been identified that constitutively localize to the kinetochore (for reviews, see Biggins and Walczak, 2003;McAinsh et al., 2003). Most of the yeast kinetochore proteins are found in biochemically distinct complexes known as the CBF3, CTF19/COMA, MTW1, NDC80, and DAM1 complexes that seem to assemble on a single centromeric nucleosome (Meluh et al., 1998). Although the exact architecture of the kinetochore is not known, dependency relationships subdivide the kinetochore into inner, central, and outer domains. The inner kinetochore contains the CBF3 complex (Ndc10, Cep3, Skp1, and Ctf13) as well as the DNA binding proteins Mif2, Cbf1, and the yeast centromeric histone H3 variant (CenH3) Cse4. CBF3 binds directly to the centromeric DNA and is thought to nucleate kinetochore assembly because all kinetochore proteins require it for localization (Russell et al., 1999;Goshima and Yanagida, 2000;He et al., 2001;Janke et al., 2001Janke et al., , 2002. The central kinetochore contains the MTW1 (Mtw1, Dsn1, Nnf1, and Nsl1) and CTF19/COMA (Ctf19, Mcm16, Mcm19, Mcm21, Mcm22, Ctf3, Chl4, Okp1, Ame1, Iml3, Nkp1, and Nkp2) complexes. CTF19/COMA can be further divided into two subcomplexes, with Ame1...

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Centromeres

Gohard

Zhiteneva

Earnshaw

2014

Encyclopedia of Life Sciences

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Cell division requires the faithful partitioning of the replicated genome into two daughter cells. Failure to achieve this can result in cell death or drive neoplastic transformation and, in meiosis, can lead to infertility and aneuploidy. Centromeres are sites at which eukaryotic chromosomes interact with the mitotic spindle and sister chromatids remain linked until properly aligned. Linkage to the spindle occurs via the kinetochore – a highly elaborate proteinaceous structure assembled on the surface of the centromere. Paradoxically given their essential functions, there is a surprising diversity in centromeric architecture, deoxyribonucleic acid (DNA) sequence and protein composition across eukaryotes. Centromere assembly and maintenance are epigenetically determined, and their key unifying feature is the presence of a centromere‐specific histone H3 variant: CENP‐A. In this article, the authors have focused on the specification, composition and function of the mammalian centromere in mitosis. Key Concepts: Centromeres are chromosomal loci necessary for chromosome segregation. The most common form of centromere is the regional centromere that is present once, and only once, per chromosome. Centromeres are epigenetically regulated; the centromere‐specific histone H3 variant CENP‐A is the determinant of centromere identity. Centromeres assemble kinetochores consisting of multiple copies of >100 proteins that regulate chromosome interactions with the mitotic spindle. Centromeres are flanked by heterochromatin, which is necessary for sister chromatid cohesion and error correction. Plasticity in centromere positioning and activity can compensate for the loss or gain of a centromere on a single chromosome fragment.

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Mis16 and Mis18 Are Required for CENP-A Loading and Histone Deacetylation at Centromeres

Cited by 393 publications

References 43 publications

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant

Centromeres

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