miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells

Zhang, Ke; Zhang, Hao; Zhou, Xun; Tang, Wenbin; Xiao, Li; Liu, Ying‐Hong; Liu, Hong; Peng, Youming; Sun, Lin; Liu, Fuyou

doi:10.1155/2012/673096

Cited by 34 publications

(31 citation statements)

References 21 publications

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“…Recent evidence suggested that miRNAs contribute greatly to EMT, especially to the EMT of PMCs. This provided critical new information that advanced the knowledge of peritoneal fibrosis [11,12]. MiRNAs are distributed in a tissue-and timespecific manner, but their roles in the MGO-induced EMT of PMCs in vivo have not been explored in depth.…”

Section: Introductionmentioning

confidence: 99%

MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats

Liu¹,

Zhang²,

Tian³

2014

Cellular and Molecular Biology Letters

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Epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMC) is a major contributor to the pathogenesis of peritoneal fibrosis. EMT is at least in part caused by repeated exposure to glucose degradation products (GDPs), such as methylglyoxal (MGO). MiRNA contributes greatly to the EMT of PMCs. In this study, we tried to profile whether differences exist between the peritoneal membrane (PM) miRNA expression seen in control rats and that seen in rats injected intraperitoneally with MGO. We assessed whether miR-30b has a possible role in MGO-induced EMT of PMCs in rats. Comparative miRNA expression array and real-time PCR analyses were conducted for the control group at the start of the experiment and for the MGO group after 1 and 2 weeks. During the second week, the MGO rats were treated with: a chemically modified antisense RNA oligonucleotide (ASO) complementary to the mature miR-30b (ASO group); an miR-30b mismatch control sequence (MIS group); or a citrate buffer (EMT group analyses indicated that the 3' untranslated region (3'-UTR) of bone morphogenetic protein 7 (BMP7) mRNA did contain a putative binding site for miR-30b. We also tried to investigate whether miR-30b targeted BMP7 in vitro by transfection. Of the upregulated miRNAs, miR-30b expression demonstrated the greatest increase. The administration of miR-30b ASO for two weeks significantly reduced α-SMA excretion and upregulated E-cadherin and BMP-7 expression. Our in vitro study showed that miR-30b directly targeted and inhibited BMP7 by binding to its 3'-UTR. Our results revealed that miR-30b is involved in MGO-induced EMT of PMCs in rats.

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Section: Introductionmentioning

confidence: 99%

MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats

Liu¹,

Zhang²,

Tian³

2014

Cellular and Molecular Biology Letters

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“…7,8 TGF- β 1 is known to have a pathogenetic role in the MMT in states of PD dysfunction. 1,10–12 However, the mechanism(s) by which TGF- β 1 induces MMT in PD dysfunction remains to be clearly defined.…”

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confidence: 99%

“…13–15 Recent studies indicate that miRNAs may contribute to the process of EMT in PD dysfunction 10,16,17 as well as in development of fibrosis. 18–20 For example, miR-30 and miR-589 have been reported to be associated with the MMT process and peritoneal fibrosis in certain models of PD 10,16 , suggesting that miRNA may h ave a critical role in the pathogenesis of TGF- β -induced fibrosis in PD.…”

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confidence: 99%

MicroRNA-129-5p modulates epithelial-to-mesenchymal transition by targeting SIP1 and SOX4 during peritoneal dialysis

Xiao

Zhou

Liu

et al. 2015

Laboratory Investigation

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Peritoneal dialysis (PD) is the most readily feasible home-dialysis method for renal replacement therapy. However, repeated use of PD can lead to induction of mesothelial/epithelial–mesenchymal transition (MMT/EMT) and fibrosis, eventually leading to ultrafiltration failure and discontinuation of PD. MicroRNA-129-5p (miR-129-5p) is believed to be a potent downstream inhibitor of TGF-β1 in renal fibrosis, but the effect of miR-129-5p on MMT/EMT relevant to PD is unknown. In this study, as determined by microRNA array analysis and confirmed by northern blot analysis and real-time PCR, we demonstrate that miRNA-129-5p is decreased in mesothelial cells isolated from effluent of patients having PD for more than 6 months extending to several years compared with those who have undergone PD for less than 6 months. The decreased expression of miR-129-5p was accompanied with alterations in EMT-related genes and the expression of respective proteins in vivo. In addition, in in vitro studies we noted that the expression of E-cadherin and claudin-1 were significantly reduced with increased cell migration in HMrSV5, a human peritoneal mesothelial cell line (HPMC), treated with TGF-β1, whereas expression of vimentin, fibronectin and transcription factors SIP1 and SOX4 increased significantly, as assessed by real-time PCR, western blot analysis and immunofluorescence microscopy. Furthermore, alteration in EMT-related genes and proteins were reversed by overexpression of miR-129-5p. No effect was observed in cells treated with miR-negative control. Meanwhile, inhibition of SIP1 and SOX4 with their respective siRNA also could decrease the expression of EMT-related genes and protein levels in HPMCs induced with TGF-β1. Finally, we demonstrate that SIP1 can inhibit the promoter activity of E-cadherin while enhancing the promoter activity of vimentin. We also observed that miR-129-5p could directly target the 3′UTR of SIP1 and SOX4 genes, and repressed their post-transcriptional activities. These data suggest that there is a novel TGF-β1/miR-129-5p/SIP-1 or SOX4 pathway that has a significant role in MMT and fibrosis in the setting of PD.

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“…Ultraviolet spectrophotometry was used to determined the content of peptides [6]. the maximum absorption peak (OD1) of polypeptide solution was determined by using ultraviolet spectrophotometer at the full wavelength to determine the maximum absorption peak (OD1) of polypeptide solution.…”

Section: Determination Of Decolorizing Ratementioning

confidence: 99%

Effect of active carbon on decoloration of antler base collagen peptides

Jiang¹

2018

Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018

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The antler base protein hydrolyze solution was decolorized through activated carbon. Based on the decolorizing rate and loss rate of peptide, the influence of peptide pH, active carbon content, treatment temperature and reaction time on the decolorization of active carbon were studied. The best conditions of decolorization were determined as follow: 2% active carbon content, pH 3, treatment temperature of 50℃, reaction time of 3h, the decolorizing rate and loss rate of antler base collagen peptides were 87.5% and 10.57±0.57%, respectively. IntroductionAntler base is the rudimental antler on the pedicle of male sika deer (Cervus nippon) or red deer (cervus elephus) after sawing off the velvet antler, which then falls off by itself when the new velvet antler begins to germinate in the next year [1,2]. It looks like a plate, so it is named 'antler base'. Antler base slag was the residue which water-soluble protein was extracted or was extracted with 50% alcohol, more than 80% of antlers base protein was retained in residue, which was mainly collagen. The collagen could be prepared antioxidant activity polypeptide used protease.More bitter flavour would be made when the more exposed hydrophobic amino groups by protease hydrolysis, at the same time, the color of hydrolysate would darken with the increase of the hydrolysis going [3]. The bitterness and yellowness of peptides solution not only affected the appearance and taste, but also had great limitations on the application of peptides. So the decolorization was an essential part in the process of purification of peptides. Activated carbon had high surface area, strong adsorptivity, low cost and no influence on the physicochemical characterization and biological activity of the product [4,5]. Therefore, active carbon was widely used in decolorizing treatment of industrial products.In this study, antler base collagen peptides were prepared by alkaline protease, the peptides were decolorized used active carbon, and the influence of decolorizing temperature, time, pH and the adding amount of activated carbon on decolorizing rate were investigated. It would provided a theoretical basis for the deep application of antlers base collagen peptide.

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miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells

Cited by 34 publications

References 21 publications

MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats

MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats

MicroRNA-129-5p modulates epithelial-to-mesenchymal transition by targeting SIP1 and SOX4 during peritoneal dialysis

Effect of active carbon on decoloration of antler base collagen peptides

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