miRNA target prediction might explain the reduced transmission of SARS-CoV-2 in Jordan, Middle East

Haddad, Hazem; Al-Zyoud, Walid

doi:10.1016/j.ncrna.2020.08.002

Cited by 16 publications

(21 citation statements)

References 9 publications

(10 reference statements)

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“… USA To understand the pathophysiology of SARS and COVID-19 and identify novel therapeutic targets through in silico miRNA analysis ( Fulzele et al, 2020 ) 6 Haddad H August 14, 2020. Jordan To determine predicted human miRNAs that to bind to the ss-RNA of the SARS-CoV-2 whole-genome and specifically to the virus spike glycoprotein gene ( Haddad and Al-Zyoud, 2020 ) 7 Srivastava R September 25, 2020. USA To understand the role of dysregulated post-transcriptional regulatory networks (RNA-binding proteins and miRNAs) during a SARS-CoV-2 infection.…”

Section: Resultsmentioning

confidence: 99%

The role of microRNAs in modulating SARS-CoV-2 infection in human cells: a systematic review

Marchi¹,

Sugita²,

Centa³

et al. 2021

Infection, Genetics and Evolution

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MicroRNAs are gene expression regulators, associated with several human pathologies, including the ones caused by virus infections. Although their role in infection diseases is not completely known, they can exert double functions in the infected cell, by mediating the virus infection and/or regulating the immunity-related gene targets through complex networks of virus-host cell interactions. In this systematic review, the Pubmed, EMBASE, Scopus, Lilacs, Scielo, and EBSCO databases were searched for research articles published until October 22nd, 2020 that focused on describing the role, function, and/or association of miRNAs in SARS-CoV-2 human infection and COVID-19. Following the PRISMA 2009 protocol, 29 original research articles were selected. Most of the studies reported miRNA data based on the genome sequencing of SARS-CoV-2 isolates and computational prediction analysis. The latter predicted, by at least one independent study, 1266 host miRNAs to target the viral genome. Thirteen miRNAs were identified by four independent studies to target SARS-CoV-2 specific genes, suggested to act by interfering with their cleavage and/or translation process. The studies selected also reported on viral and host miRNAs that targeted host genes, on the expression levels of miRNAs in biological specimens of COVID-19 patients, and on the impact of viral genome mutations on miRNA function. Also, miRNAs that regulate the expression levels of the ACE2 and TMPRSS2 proteins, which are critical for the virus entrance in the host cells, were reported. In conclusion, despite the limited number of studies identified, based on the search terms and eligibility criteria applied, this systematic review provides evidence on the impact of miRNAs on SARS-CoV-2 infection and COVID-19. Although most of the reported viral/host miRNAs interactions were based on in silico prediction analysis, they demonstrate the relevance of the viral/host miRNA interaction for viral activity and host responses. In addition, the identified studies highlight the potential use of miRNAs as therapeutic targets against COVID-19, and other viral human diseases (This review was registered at the International Prospective Register of Systematic Reviews (PROSPERO) database (#CRD42020199290).

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Section: Resultsmentioning

confidence: 99%

The role of microRNAs in modulating SARS-CoV-2 infection in human cells: a systematic review

Marchi¹,

Sugita²,

Centa³

et al. 2021

Infection, Genetics and Evolution

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show abstract

“…Therefore, the mutant variant could cause a more severe condition due to the absence of the hsa-miR-27b-3p sequence [31]. An amino acid substation of D614G at the S glycoprotein changed its miRNA sequence from hsa-miR-4793-5p to hsa-miR-3620-3p and increased the target score due to the nucleotide substitution 1841A > G at the SARS-CoV-2 RNA [32]. The artificial regulation of human and viral miRNAs could also change COVID-19 pathophysiology.…”

Section: Discussionmentioning

confidence: 99%

“…hsa-miR-23b and hsa-miR-98, by cleaving VP1 and interferon (IFN)-β genes, target the S protein of SARS-CoV-2 [31]. hsa-miR-510-3p, hsa-miR-624-5p, and hsa-miR-497-5p have been reported to target RNA S glycoprotein of SARS-CoV-2 sequence [32]. In addition, hsa-miR-622, hsa-miR-761, miR-A3r, hsa-miR-15b-5p, miR-A2r, and has-miR-196a-5p have been shown to target the S protein gene [33].…”

Section: Attachment and Entrymentioning

confidence: 99%

“…hsa-let-7a and hsa-miR-101 have been shown to target nonstructural protein synthesis in SARS-CoV-2, by cleaving IFN-β and ATP5B genes, respectively [31]. hsa-miR-497-5p, hsa-miR -21-3p, and hsa-miR-195-5p are widely expressed in respiratory epithelial cells, have a high target score against the SARS-CoV-2 genome, and are predicted to be effective against the virus through nucleotide deletion in the coding regions of the viral ss-RNA [32].…”

Section: Viral Genomic Replication and Translation And Protein Synthesismentioning

confidence: 99%

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MicroRNAs and SARS-CoV-2 life cycle, pathogenesis, and mutations: biomarkers or therapeutic agents?

et al. 2020

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To date, proposed therapies and antiviral drugs have been failed to cure coronavirus disease 2019 (COVID-19) patients. However, at least two drug companies have applied for emergency use authorization with the United States Food and Drug Administration for their coronavirus vaccine candidates and several other vaccines are in various stages of development to determine safety and efficacy. Recently, some studies have shown the role of different human and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) microRNAs (miRNAs) in the pathophysiology of COVID-19. miRNAs are non-coding single-stranded RNAs, which are involved in several physiological and pathological conditions, such as cell proliferation, differentiation, and metabolism. They act as negative regulators of protein synthesis through binding to the 3′ untranslated region (3′ UTR) of the complementary target mRNA, leading to mRNA degradation or inhibition. The databases of Google Scholar, Scopus, PubMed, and Web of Science were searched for literature regarding the importance of miRNAs in the SARS-CoV-2 life cycle, pathogenesis, and genomic mutations. Furthermore, promising miRNAs as a biomarker or antiviral agent in COVID-19 therapy are reviewed.

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“…However, we have recently reported in Non-coding RNA Research that the 1841A > G substitution at the viral genomic RNA level (D614G at spike protein level) showed many named microRNA sequences in human cells (e.g., hsa-miR-4793-5p to hsa-miR-3620-3p). These named miRNA sequences with an increased target binding score with viral RNA of spike protein from 91% to 92% by utilizing the miRDB database [ 21 ]. Our non-coding RNA findings support a new hypothesis of host non-coding RNA based response, which can directly bind and affect the viral RNA replication of the D614G spike protein.…”

Section: Discussionmentioning

confidence: 99%

Mutational sensitivity of D614G in spike protein of SARS-CoV-2 in Jordan

Al-Zyoud

Haddad

2021

Biochemistry and Biophysics Reports

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miRNA target prediction might explain the reduced transmission of SARS-CoV-2 in Jordan, Middle East

Cited by 16 publications

References 9 publications

The role of microRNAs in modulating SARS-CoV-2 infection in human cells: a systematic review

The role of microRNAs in modulating SARS-CoV-2 infection in human cells: a systematic review

MicroRNAs and SARS-CoV-2 life cycle, pathogenesis, and mutations: biomarkers or therapeutic agents?

Mutational sensitivity of D614G in spike protein of SARS-CoV-2 in Jordan

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