miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing

Shinohara, Yuta; Yahagi, Kazuko; Kawano, Mitsuoki; Nishiyori, Hiromi; Kawazu, Chika; Suzuki, Naoki; Manabe, Ri Ichiroh; Hirase, Hajime

doi:10.1016/j.bbrc.2011.05.004

Cited by 17 publications

(21 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A–C). The founding member of the miRNA family let-7 and mir-125 are highly expressed in neurons consistent with previous data [18], [40], [41], [68] (Fig. 2A–C).…”

Section: Resultssupporting

confidence: 92%

“…Selective inactivation of Dicer during hippocampal development causes abnormal neuronal morphology and affects the number of hippocampal progenitors [29] and results in an array of phenotypes including reduced dendritic branching, and large increases in dendritic spine length in mature neurons [26]. Using microarray, real-time RT-PCR, in situ hybridization, and deep-sequencing approaches, several studies have identified miRNAs in the hippocampus during development or in adulthood [15], [18], [40], [41], after induction of neuronal activity [42], [43], [44] or injury [45], [46]. While most of these studies where performed in hippocampal brain tissue or slices, in this study we focus on primary rat hippocampal neurons in culture and analyze the miRNA expression profiles during development and after induction of neuronal activity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures

et al. 2013

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are evolutionarily conserved non-coding RNAs of ∼22 nucleotides that regulate gene expression at the level of translation and play vital roles in hippocampal neuron development, function and plasticity. Here, we performed a systematic and in-depth analysis of miRNA expression profiles in cultured hippocampal neurons during development and after induction of neuronal activity. MiRNA profiling of primary hippocampal cultures was carried out using locked nucleic-acid-based miRNA arrays. The expression of 264 different miRNAs was tested in young neurons, at various developmental stages (stage 2–4) and in mature fully differentiated neurons (stage 5) following the induction of neuronal activity using chemical stimulation protocols. We identified 210 miRNAs in mature hippocampal neurons; the expression of most neuronal miRNAs is low at early stages of development and steadily increases during neuronal differentiation. We found a specific subset of 14 miRNAs with reduced expression at stage 3 and showed that sustained expression of these miRNAs stimulates axonal outgrowth. Expression profiling following induction of neuronal activity demonstrates that 51 miRNAs, including miR-134, miR-146, miR-181, miR-185, miR-191 and miR-200a show altered patterns of expression after NMDA receptor-dependent plasticity, and 31 miRNAs, including miR-107, miR-134, miR-470 and miR-546 were upregulated by homeostatic plasticity protocols. Our results indicate that specific miRNA expression profiles correlate with changes in neuronal development and neuronal activity. Identification and characterization of miRNA targets may further elucidate translational control mechanisms involved in hippocampal development, differentiation and activity-depended processes.

show abstract

“…2A–C). The founding member of the miRNA family let-7 and mir-125 are highly expressed in neurons consistent with previous data [18], [40], [41], [68] (Fig. 2A–C).…”

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures

et al. 2013

View full text Add to dashboard Cite

show abstract

“…General profiling by microarrays distinguished the differential expression of miRNAs in different regions of the rodent brain [49,50] with a specific emphasis on the cortex and hippocampus [51,52]. More recently, miRNA expression was also explored under conditions of elevated activity known to induce synaptic plasticity.…”

Section: Mirna Function In Synaptic Plasticity With Implications For mentioning

confidence: 99%

MicroRNAs and synaptic plasticity—a mutual relationship

Aksoy‐Aksel

Zampa

Schratt

2014

Phil. Trans. R. Soc. B

105

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are rapidly emerging as central regulators of gene expression in the postnatal mammalian brain. Initial studies mostly focused on the function of specific miRNAs during the development of neuronal connectivity in culture, using classical gain-and loss-of-function approaches. More recently, first examples have documented important roles of miRNAs in plastic processes in intact neural circuits in the rodent brain related to higher cognitive abilities and neuropsychiatric disease. At the same time, evidence is accumulating that miRNA function itself is subjected to sophisticated control mechanisms engaged by the activity of neural circuits. In this review, we attempt to pay tribute to this mutual relationship between miRNAs and synaptic plasticity. In particular, in the first part, we summarize how neuronal activity influences each step in the lifetime of miRNAs, including the regulation of transcription, maturation, gene regulatory function and turnover in mammals. In the second part, we discuss recent examples of miRNA function in synaptic plasticity in rodent models and their implications for higher cognitive function and neurological disorders, with a special emphasis on epilepsy as a disorder of abnormal nerve cell activity.

show abstract

“…Most profiling studies use a fold-change cut-off but this may not be appropriate for very abundant miRNAs where this would potentially miss changes in the order of thousands of copies per cell. Notably, the most abundant miRNAs in the hippocampus, such as members of the let-7 family, miR-124 and miR-9 [41-42] often do not appear in the miRNA lists in epilepsy studies (see Table 1) and researchers should be encouraged to re-mine their data with this in mind.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA and epilepsy

Henshall

2014

Current Opinion in Neurology

107

View full text Add to dashboard Cite

Purpose of Review To provide a synthesis of recent profiling studies investigating microRNA changes in experimental and human epilepsy and outline mechanistic, therapeutic and diagnostic potentials of this research area for clinical practice. Recent Findings A series of studies in experimental and human epilepsy have undertaken large-scale expression profiling of microRNAs, key regulatory molecules in cells controlling protein levels. Levels of over 100 different microRNAs were found to either increase or decrease in the hippocampus, of which more than 20 were identified in more than one study, including higher levels of miR-23a, miR-34a, miR-132 and miR-146a. Altered levels of enzymes involved in microRNA biogenesis and function, including Dicer and Argonaute 2, have also been found in epileptic brain tissue. Functional studies using oligonucleotide-based inhibitors support roles for microRNAs in the control of cell death, synaptic structure, inflammation and the immune response. Finally, data show brain injuries that precipitate epilepsy generate unique microRNA profiles in biofluids. Summary MicroRNA represents a potentially important mechanism controlling protein levels in epilepsy. As such, microRNAs might be targeted to prevent or disrupt epilepsy as well as serve as diagnostic biomarkers of epileptogenesis.

show abstract

miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing

Cited by 17 publications

References 29 publications

Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures

Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures

MicroRNAs and synaptic plasticity—a mutual relationship

MicroRNA and epilepsy

Contact Info

Product

Resources

About