miRNA profiling in the hippocampus of attention‐deficit/hyperactivity disorder rats

Tian, Tian; Zhang, Yan; Wu, Tianqi; Yang, Lei; Chen, Chunxiao; Li, Nan; Li, Yue; Xu, Siliang; Fu, Ziyi; Ji, Chenbo; Chi, Xia; Tong, Meiling; Chen, Ronghua; Qin, Hong; Hu, Yong

doi:10.1002/jcb.27639

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…We found that 17 miRNAs whose expression was changed in mice hippocampus by BPA. Bioinformatics analysis was conducted to further study the potential roles of the target genes regulated by these deregulated miRNAs [ 47 ]. GO analysis suggested that target genes of these miRNAs were closely associated with regulation of central nervous system structure and function, including several cognitive processes, especially the learning and memory.…”

Section: Discussionmentioning

confidence: 99%

Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis

Luo

Ding

et al. 2023

PLoS ONE

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The mechanisms of Bisphenol A (BPA) induced learning and memory impairment have still not been fully elucidated. MicroRNAs (miRNAs) are endogenous non-coding small RNA molecules involved in the process of toxicant-induced neurotoxicity. To investigate the role of miRNAs in BPA-induced learning and memory impairment, we analyzed the impacts of BPA on miRNA expression profile by high-throughput sequencing in mice hippocampus. Results showed that mice treated with BPA displayed impairments of spatial learning and memory and changes in the expression of miRNAs in the hippocampus. Seventeen miRNAs were significantly differentially expressed after BPA exposure, of these, 13 and 4 miRNAs were up- and downregulated, respectively. Bioinformatic analysis of Gene Ontology (GO) and pathway suggests that BPA exposure significantly triggered transcriptional changes of miRNAs associated with learning and memory; the top five affected pathways involved in impairment of learning and memory are: 1) Long-term depression (LTD); 2) Thyroid hormone synthesis; 3) GnRH signaling pathway; 4) Long-term potentiation (LTP); 5) Serotonergic synapse. Eight BPA-responsive differentially expressed miRNAs regulating LTP and LTD were further screened to validate the miRNA sequencing data using Real-Time PCR. The deregulation expression levels of proteins of five target genes (CaMKII, MEK1/2, IP3R, AMPAR1 and PLCβ4) were investigated via western blot, for further verifying the results of gene target analysis. Our results showed that LTP and LTD related miRNAs and their targets could contribute to BPA-induced impairment of learning and memory. This study provides valuable information for novel miRNA biomarkers to detect changes in impairment of learning and memory induced by BPA exposure.

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Section: Discussionmentioning

confidence: 99%

Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis

Luo

Ding

et al. 2023

PLoS ONE

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“…For example, miR-764-3p was down-regulated in the hippocampus of rats with dementia [ 70 ]. miR-741-3p was upregulated in attention-deficit/hyperactivity disorder rats [ 71 ]. miR-18a-5p was involved in axonogenesis in rat hippocampus by regulating its target genes [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Analysis of Repeated Low-Level Sarin-Exposed Rat Hippocampus and Identification of Cerna Networks to Investigate the Mechanism of Sarin-Induced Cognitive Impairment

Shi

Liu

Jin

et al. 2023

Biology

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Sarin is a potent organophosphorus nerve agent that causes cognitive dysfunction, but its underlying molecular mechanisms are poorly understood. In this study, a rat model of repeated low-level sarin exposure was established using the subcutaneous injection of 0.4 × LD50 for 21 consecutive days. Sarin-exposed rats showed persistent learning and memory impairment and reduced hippocampal dendritic spine density. A whole-transcriptome analysis was applied to study the mechanism of sarin-induced cognitive impairment, and a total of 1035 differentially expressed mRNA (DEmRNA), including 44 DEmiRNA, 305 DElncRNA, and 412 DEcircRNA, were found in the hippocampus of sarin-treated rats. According to Gene Ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and Protein–Protein Interaction (PPI) analysis, these DERNAs were mainly involved in neuronal synaptic plasticity and were related to the pathogenesis of neurodegenerative diseases. The circRNA/lncRNA–miRNA–mRNA ceRNA network was constructed, in which Circ_Fmn1, miR-741-3p, miR-764-3p, miR-871-3p, KIF1A, PTPN11, SYN1, and MT-CO3 formed one circuit, and Circ_Cacna1c, miR-10b-5p, miR-18a-5p, CACNA1C, PRKCD, and RASGRP1 constituted another circuit. The balance between the two circuits was crucial for maintaining synaptic plasticity and may be the regulatory mechanism by which sarin causes cognitive impairment. Our study reveals the ceRNA regulation mechanism of sarin exposure for the first time and provides new insights into the molecular mechanisms of other organophosphorus toxicants.

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“…miR-741-3p is a member of the fragile-X miRNA cluster [ 225 ] and was dramatically upregulated in rat models of nonalcoholic fatty liver disease [ 226 ] and attention-deficit/hyperactivity disorder [ 227 ]. A previous study showed protection of miR-741-3p against radiation-induced injury of bone mesenchymal stem cells [ 228 ].…”

Section: Modulation Of Microglia For Ribi Therapymentioning

confidence: 99%

Microglia as Therapeutic Target for Radiation-Induced Brain Injury

Liu

Yan

Duan

et al. 2022

IJMS

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Radiation-induced brain injury (RIBI) after radiotherapy has become an increasingly important factor affecting the prognosis of patients with head and neck tumor. With the delivery of high doses of radiation to brain tissue, microglia rapidly transit to a pro-inflammatory phenotype, upregulate phagocytic machinery, and reduce the release of neurotrophic factors. Persistently activated microglia mediate the progression of chronic neuroinflammation, which may inhibit brain neurogenesis leading to the occurrence of neurocognitive disorders at the advanced stage of RIBI. Fully understanding the microglial pathophysiology and cellular and molecular mechanisms after irradiation may facilitate the development of novel therapy by targeting microglia to prevent RIBI and subsequent neurological and neuropsychiatric disorders.

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miRNA profiling in the hippocampus of attention‐deficit/hyperactivity disorder rats

Cited by 14 publications

References 37 publications

Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis

Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis

Whole-Transcriptome Analysis of Repeated Low-Level Sarin-Exposed Rat Hippocampus and Identification of Cerna Networks to Investigate the Mechanism of Sarin-Induced Cognitive Impairment

Microglia as Therapeutic Target for Radiation-Induced Brain Injury

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