miRNA–mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress

Zhang, Huilong; Liu, Xiaowei; Yang, Xiuyan; Wu, Haoran; Zhu, Jianfeng; Zhang, Huaxin

doi:10.3390/plants9030351

Cited by 22 publications

(16 citation statements)

References 66 publications

(74 reference statements)

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“…In a typical halophyte, Reaumuria soongorica, 13 novel miRNAs were discovered under salt stress. miRNA-mRNA integrated analysis revealed that miRNAs regulate the network response to salt stress during seed germination through GA, auxin, and ABA signaling pathways (Zhang H. et al, 2020). In addition, a comparative transcriptome analysis revealed some new lncRNAs in sweet sorghum, including lncRNA13472, lncRNA11310, lncRNA2846, lncRNA26929, and lncRNA14798.…”

Section: Epigenetic Changesmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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As two coexisting abiotic stresses, salt stress and alkali stress have severely restricted the development of global agriculture. Clarifying the plant resistance mechanism and determining how to improve plant tolerance to salt stress and alkali stress have been popular research topics. At present, most related studies have focused mainly on salt stress, and salt-alkali mixed stress studies are relatively scarce. However, in nature, high concentrations of salt and high pH often occur simultaneously, and their synergistic effects can be more harmful to plant growth and development than the effects of either stress alone. Therefore, it is of great practical importance for the sustainable development of agriculture to study plant resistance mechanisms under saline-alkali mixed stress, screen new saline-alkali stress tolerance genes, and explore new plant salt-alkali tolerance strategies. Herein, we summarized how plants actively respond to saline-alkali stress through morphological adaptation, physiological adaptation and molecular regulation.

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Section: Epigenetic Changesmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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“…Reaumuria soongorica is a typical perennial salt-yielding halophytic shrub, which shows strong adaptability to saline and desert soils, and is a representative dominant species in salt-alkali desert areas of grassland ecosystems. This species plays a crucial role in stabilizing shifting sands, thereby helping to prevent soil erosion and desertification 17 . Further, the R. soongorica community provides a good pasture in desert areas 18 , with excellent soil improvement effects 19 .…”

Section: Introductionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s response to salinity have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was positively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47 177 136 differentially expressed proteins (DEPs) were identified in low-salt versus CK, high-salt versus control, and high-salt versus low-salt comparisons, respectively. A total of 72 DEPs were further screened from the comparison groupings, of which 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 points) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated versus CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis for enhancing its breeding, under salt stress conditions.

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“…14). Of the 15 identified mun-miRs, miR 156, miR 171 and miR 390 were found to be differentially expressed in germinating seeds of halophyte Reaumuria soongorica under salt stress conditions (54). Legume genomes are rich in SNPs.…”

Section: Discussionmentioning

confidence: 99%

Genome wide in-silico miRNA and target network prediction from stress responsive Horsegram (Macrotyloma uniflorum) accessions

Yasin

Mishra

Pillai

et al. 2020

Sci Rep

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Horsegram (Macrotyloma uniflorum (Lam.) Verdc.) is a drought hardy food and fodder legume of Indo-African continents with diverse germplasm sources demonstrating alternating mechanisms depicting contrasting adaptations to different climatic zones. Tissue specific expression of genes contributes substantially to location specific adaptations. Regulatory networks of such adaptive genes are elucidated for downstream translational research. MicroRNAs are small endogenous regulatory RNAs which alters the gene expression profiles at a particular time and type of tissue. Identification of such small regulatory RNAs in low moisture stress hardy crops can help in cross species transfer and validation confirming stress tolerance ability. This study outlined prediction of conserved miRNAs from transcriptome shotgun assembled sequences and EST sequences of horsegram. We could validate eight out of 15 of the identified miRNAs to demonstrate their role in deficit moisture stress tolerance mechanism of horsegram variety Paiyur1 with their target networks. The putative mumiRs were related to other food legumes indicating the presence of gene regulatory networks. Differential miRNA expression among drought specific tissues indicted the probable energy conservation mechanism. Targets were identified for functional characterization and regulatory network was constructed to find out the probable pathways of post-transcriptional regulation. The functional network revealed mechanism of biotic and abiotic stress tolerance, energy conservation and photoperiod responsiveness.

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miRNA–mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress

Cited by 22 publications

References 66 publications

Response Mechanisms of Plants Under Saline-Alkali Stress

Response Mechanisms of Plants Under Saline-Alkali Stress

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Genome wide in-silico miRNA and target network prediction from stress responsive Horsegram (Macrotyloma uniflorum) accessions

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