miRNA let-7 family regulated by NEAT1 and ARID3A/NF-κB inhibits PRRSV-2 replication in vitro and in vivo

You, Xiangbin; Liu, Min; Liu, Qian; Li, Huijuan; Qu, Yilin; Gao, Xiaoxiao; Huang, Chengyu; Luo, Gan; Cao, Gang; Xu, Dequan

doi:10.1371/journal.ppat.1010820

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…Meng cloned the GP5 gene into a eukaryotic expression plasmid under the control of the cytomegalovirus early promoter to prepare a DNA vaccine, which could induce the production of antibodies after immunization of piglets [ 41 ]. More and more studies showed that miRNAs could be used to treat PRRS [ 42 – 44 ]. In our research, intramuscular injection of pEGFP-N1-miR-320 could reduce the replication of PRRSV in pigs.…”

Section: Discussionmentioning

confidence: 99%

MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB

Gao,

You,

Wang

et al. 2024

Vet Res

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Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease caused by Porcine reproductive and respiratory syndrome virus (PRRSV), results in huge economic losses to the world pig industry. MiRNAs have been reported to be involved in regulation of viral infection. In our study, miR-320 was one of 21 common differentially expressed miRNAs of Meishan, Pietrain, and Landrace pig breeds at 9-h post-infection (hpi). Bioinformatics and experiments found that PRRSV replication was inhibited by miR-320 through directly targeting PRRSV ORF6. In addition, the expression of CCAAT enhancer binding protein beta (CEBPB) was also inhibited by miR-320 by targeting the 3ʹ UTR of CEBPB, which significantly promotes PRRSV replication. Intramuscular injection of pEGFP-N1-miR-320 verified that miR-320 significantly inhibited the replication of PRRSV and alleviated the symptoms caused by PRRSV in piglets. Taken together, miR-320 have significant roles in the infection and may be promising therapeutic target for PRRS.

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Section: Discussionmentioning

confidence: 99%

MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB

Gao,

You,

Wang

et al. 2024

Vet Res

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“…Accumulating evidence has confirmed that let-7 plays a key role in regulating immune responses ( 92 , 93 ). Upon PRRSV infection, the expression of let-7 family is significantly upregulated, and the let-7 family inhibits PRRSV proliferation via directly targeting both the PRRSV-2 genome and IL-6 ( Figure 2A , Table 1 ) ( 16 ). More importantly, let-7 family can remarkably decrease PRRSV infection and associated pathological changes both in vitro and in vivo , suggesting that the let-7 family may serve as promising therapeutic targets for PRRS.…”

Section: Roles Of Mirnas In Host Response To Prrsv Infectionmentioning

confidence: 99%

“…Mature miRNAs, within the RISC, induce translational repression and deadenylation of target mRNA. Numerous host miRNAs have demonstrated influence on various processes, including pathogenic diseases and host immunity ( 4 , 15 , 16 ). Therefore, understanding the biological functions of miRNAs and the mechanism of their actions will be instrumental in drug development and disease control.…”

Section: Introductionmentioning

confidence: 99%

Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line

Huang,

Liu

2024

Front. Immunol.

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Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most globally devastating viruses threatening the swine industry worldwide. Substantial advancements have been achieved in recent years towards comprehending the pathogenesis of PRRSV infection and the host response, involving both innate and adaptive immune responses. Not only a multitude of host proteins actively participate in intricate interactions with viral proteins, but microRNAs (miRNAs) also play a pivotal role in the host response to PRRSV infection. If a PRRSV–host interaction at the protein level is conceptualized as the front line of the battle between pathogens and host cells, then their fight at the RNA level resembles the hidden front line. miRNAs are endogenous small non-coding RNAs of approximately 20–25 nucleotides (nt) that primarily regulate the degradation or translation inhibition of target genes by binding to the 3’-untranslated regions (UTRs). Insights into the roles played by viral proteins and miRNAs in the host response can enhance our comprehensive understanding of the pathogenesis of PRRSV infection. The intricate interplay between viral proteins and cellular targets during PRRSV infection has been extensively explored. This review predominantly centers on the contemporary understanding of the host response to PRRSV infection at the RNA level, in particular, focusing on the twenty-six miRNAs that affect viral replication and the innate immune response.

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“…The let-7 family consists of let-7a, let-7c, let-7d, let-7e, let-7f, let-7g, let-7i, and miR-98. A recent study has demonstrated that PRRSV-2 infection leads to a marked change in the expression of the let-7 family, which subsequently targets PRRSV-2 genomic RNAs, effectively inhibiting their replication [ 121 ]. Moreover, the expression of ARID3A transcription factor down-regulates let-7a and let-7f expression, thereby significantly promoting PRRSV-2 replication.…”

Section: Ncrna During Porcine Virus Infectionmentioning

confidence: 99%

Regulatory Non-Coding RNAs during Porcine Viral Infections: Potential Targets for Antiviral Therapy

Li,

Yu,

et al. 2024

Viruses

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Pigs play important roles in agriculture and bio-medicine; however, porcine viral infections have caused huge losses to the pig industry and severely affected the animal welfare and social public safety. During viral infections, many non-coding RNAs are induced or repressed by viruses and regulate viral infection. Many viruses have, therefore, developed a number of mechanisms that use ncRNAs to evade the host immune system. Understanding how ncRNAs regulate host immunity during porcine viral infections is critical for the development of antiviral therapies. In this review, we provide a summary of the classification, production and function of ncRNAs involved in regulating porcine viral infections. Additionally, we outline pathways and modes of action by which ncRNAs regulate viral infections and highlight the therapeutic potential of artificial microRNA. Our hope is that this information will aid in the development of antiviral therapies based on ncRNAs for the pig industry.

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miRNA let-7 family regulated by NEAT1 and ARID3A/NF-κB inhibits PRRSV-2 replication in vitro and in vivo

Cited by 14 publications

References 47 publications

MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB

MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB

Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line

Regulatory Non-Coding RNAs during Porcine Viral Infections: Potential Targets for Antiviral Therapy

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