miRNA for the assessment of lymph node metastasis in patients with oral squamous cell carcinoma: Systematic review and metanalysis

Abstract

Background miRNA is one of the advanced epigenetic molecular markers correlating with lymph node metastasis in patients with Oral squamous cell carcinoma (OSCC). Numerous published papers are showing correlation of miRNA with metastasis. There is a need to analyze and validate such correlation. Method English language literature in major databases from the last 20 years was searched using controlled vocabulary and keywords. Strict inclusion and exclusion criteria were followed for selection of studies. The quality assessment was done as per the QUADAS tool 2 by three independent reviewers. The metanalysis was performed by using random effect model. Standardized mean difference (SMD) was considered as the effect measure. Statistical software used was STATA version 13.1. Results With all inclusion and exclusion criteria, eight studies could qualify for metanalysis. The pooled estimate is found to be 0.13 (−0.35, 0.62), P = .585, which is statistically not significant. This indicates that there is a no significant difference in the fold change between metastasis and no metastasis groups. P‐value of chi‐square statistic for heterogeneity is <.001 (significant), and I‐squared statistic is 87.2%, which indicates that heterogeneity is present to a considerable extent. Egger's test shows there is no publication bias involved (P = .819). Conclusion The metanalysis showed no significant difference in the fold change of miRNA expression between metastasis and non‐metastasis OSCC patients. Future studies can be directed to eliminate the heterogeneity among the studies noted in this analysis to confirm the role of miRNA for assessment of regional metastasis with special focus on tongue squamous cell carcinoma.

lncRNA HOXA11‐AS maintains the stemness of oral squamous cell carcinoma stem cells and reduces the radiosensitivity by targeting miR‐518a‐3p/PDK1

“…miRNAs are epigenetic molecular markers associated with lymph node metastasis in OSCC patients. 26 Expectedly, knockdown of miR-518a-3p partially reversed the effects of HOXA11-AS silencing on F I G U R E 4 HOXA11-AS silencing inhibited EMT in CSCs. HOXA11-AS was knocked down or overexpressed in CD133 + SCC15 and CD133 + SCC9 cells, respectively.…”

“…miRNAs are epigenetic molecular markers associated with lymph node metastasis in OSCC patients 26 . Expectedly, knockdown of miR‐518a‐3p partially reversed the effects of HOXA11‐AS silencing on repressing CSC stemness and enhancing cell radiosensitivity, indicating that HOXA11‐AS modulated the stem cell stemness and radiosensitivity of OSCC by targeting miR‐518a‐3p.…”

Salivary Biomarkers for Oral Cancer Detection: An Exploratory Systematic Review

“…The study of miRNA holds promise. Different systematic reviews have also concluded that they are potential biomarkers for OSCC and OPMDs [ 11 , 72 , 73 ]. The main issue is, as with many of the other markers, is that apart from mir-21, which was assessed by two studies [ 47 , 48 ], the salivary expression of all other miRNAs, has been analyzed by single studies.…”

Expression of MicroRNAs in Sepsis-Related Organ Dysfunction: A Systematic Review

“…In recent years, miRNAs have taken a prominent role in the study of non-coding RNAs [ 281 ]. Many studies have focused on the role of miRNAs in the pathophysiology of various diseases such as cancer, and neurodegenerative and immune-mediated diseases [ 282 , 283 , 284 , 285 , 286 ]. As knowledge has advanced, it has become apparent that the most important targets of miRNAs are the genes that regulate the complicated inflammatory process [ 287 , 288 , 289 ].…”