miRNA expression in COVID-19

Geraylow, Kiarash Roustai; Hemmati, Romina; Kadkhoda, Sepideh; Ghafouri‐Fard, Soudeh

doi:10.1016/j.genrep.2022.101641

Cited by 14 publications

(13 citation statements)

References 89 publications

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“…We used Calu-3, a common, sensitive and effective preclinical model for studying human respiratory processes and lung-injury-related diseases [ 52 ]. Calu-3 is a well-established in vitro model of respiratory virus infection, an alternative approach that mimics the characteristics of bronchial epithelial cells very well [ 53 , 54 ]. However, Calu-3 is a lineage derived from lung adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Altered microRNA Transcriptome in Cultured Human Airway Cells upon Infection with SARS-CoV-2

Diallo

Jacob

Vion

et al. 2023

Viruses

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Numerous proteomic and transcriptomic studies have been carried out to better understand the current multi-variant SARS-CoV-2 virus mechanisms of action and effects. However, they are mostly centered on mRNAs and proteins. The effect of the virus on human post-transcriptional regulatory agents such as microRNAs (miRNAs), which are involved in the regulation of 60% of human gene activity, remains poorly explored. Similar to research we have previously undertaken with other viruses such as Ebola and HIV, in this study we investigated the miRNA profile of lung epithelial cells following infection with SARS-CoV-2. At the 24 and 72 h post-infection time points, SARS-CoV-2 did not drastically alter the miRNome. About 90% of the miRNAs remained non-differentially expressed. The results revealed that miR-1246, miR-1290 and miR-4728-5p were the most upregulated over time. miR-196b-5p and miR-196a-5p were the most downregulated at 24 h, whereas at 72 h, miR-3924, miR-30e-5p and miR-145-3p showed the highest level of downregulation. In the top significantly enriched KEGG pathways of genes targeted by differentially expressed miRNAs we found, among others, MAPK, RAS, P13K-Akt and renin secretion signaling pathways. Using RT-qPCR, we also showed that SARS-CoV-2 may regulate several predicted host mRNA targets involved in the entry of the virus into host cells (ACE2, TMPRSS2, ADAM17, FURIN), renin–angiotensin system (RAS) (Renin, Angiotensinogen, ACE), innate immune response (IL-6, IFN1β, CXCL10, SOCS4) and fundamental cellular processes (AKT, NOTCH, WNT). Finally, we demonstrated by dual-luciferase assay a direct interaction between miR-1246 and ACE-2 mRNA. This study highlights the modulatory role of miRNAs in the pathogenesis of SARS-CoV-2.

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Section: Discussionmentioning

confidence: 99%

Altered microRNA Transcriptome in Cultured Human Airway Cells upon Infection with SARS-CoV-2

Diallo

Jacob

Vion

et al. 2023

Viruses

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“…Teo et al, in their study with rs8679, found that the total ΔΔG value for miRNA binding was lower in the variant allele versus the wild type ( Teo et al, 2012 ). Many miRNA dysregulations have been demonstrated in patients with COVID-19 ( Paul et al, 2022 ; Roustai Geraylow et al, 2022 ). Demiray et al found that miR-223 decreased in COVID-19 patients compared to healthy controls ( Demiray et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The 3′UTR region of the DNA repair gene PARP-1 May increase the severity of COVID-19 by altering the binding of antiviral miRNAs

et al. 2023

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“…45 Since the start of COVID-19, several studies have investigated the possible dysregulation of human miRNAs after SARS-CoV-2 infection in vivo, in vitro, and in silico [46][47][48] Initially, human miRNAs were predicted using computer-assisted techniques that were further validated through in vivo or in vitro studies. [48][49][50] In terms of miRNAs related to SARS-CoV-2/COVID-19, within a short span of only ~2 years, already hundreds of studies have been published. In this systematic review, we not only summarise the currently available data, but also analyse the available data from patients to examine the performance of currently enumerated miRNAs as biomarkers across the globe.…”

Section: Mirnas In Sars-cov-2mentioning

confidence: 99%

“…Moreover, viruses also transcribe miRNAs that may interfere with the host‐cellular defence system 45 . Since the start of COVID‐19, several studies have investigated the possible dysregulation of human miRNAs after SARS‐CoV‐2 infection in vivo, in vitro, and in silico 46–48 Initially, human miRNAs were predicted using computer‐assisted techniques that were further validated through in vivo or in vitro studies 48–50 . In terms of miRNAs related to SARS‐CoV‐2/COVID‐19, within a short span of only ∼2 years, already hundreds of studies have been published.…”

Section: Introductionmentioning

confidence: 99%

Differentially‐regulated miRNAs in COVID‐19: A systematic review

Ahmad

Gull

Baby

et al. 2023

Reviews in Medical Virology

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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for coronavirus disease of 2019 that infected more than 760 million people worldwide with over 6.8 million deaths to date. COVID-19 is one of the most challenging diseases of our times due to the nature of its spread, its effect on multiple organs, and an inability to predict disease prognosis, ranging from being completely asymptomatic to death. Upon infection, SARS-CoV-2 alters the host immune response by changing host-transcriptional machinery. MicroRNAs (miRNAs) are regarded as post-transcriptional regulators of gene expression that can be perturbed by invading viruses. Several in vitro and in vivo studies have reported such dysregulation of host miRNA expression upon SARS-CoV-2 infection. Some of this could occur as an anti-viral response of the host to the viral infection. Viruses themselves can counteract that response by mounting their own pro-viral response that facilitates virus infection, an aspect which may cause pathogenesis. Thus, miRNAs could serve as possible disease biomarkers in infected people. In the current review, we have summarised and analysed the existing data about miRNA dysregulation in patients infected with SARS-CoV-2 to determine their concordance between studies, and identified those that could serve as potential biomarkers during infection, disease progression, and death, even in people with other comorbidities. Having such biomarkers can be vital in not only predicting prognosis, but also the development of novel miRNA-based anti-virals and Abbreviations: 3 0 UTR, 3 0 untranslated regions; 5 0 UTR, 5 0

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miRNA expression in COVID-19

Cited by 14 publications

References 89 publications

Altered microRNA Transcriptome in Cultured Human Airway Cells upon Infection with SARS-CoV-2

Altered microRNA Transcriptome in Cultured Human Airway Cells upon Infection with SARS-CoV-2

The 3′UTR region of the DNA repair gene PARP-1 May increase the severity of COVID-19 by altering the binding of antiviral miRNAs

Differentially‐regulated miRNAs in COVID‐19: A systematic review

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