miRNA Clusters with Down-Regulated Expression in Human Colorectal Cancer and Their Regulation

Pidíková, Paulína; Reis, Richard M.; Herichová, Iveta

doi:10.3390/ijms21134633

Cited by 51 publications

(33 citation statements)

References 176 publications

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“…miRNA-mediated gene expression regulation involves many biological backgrounds, such as cell differentiation, proliferation, apoptosis, and carcinogenesis [17][18][19][20][21] complementary sites on the target mRNA, resulting in the negative or positive regulation of the expression of the target gene [22][23][24][25][26]. e low expression of many miRNA clusters in CRC regulates proliferation and metastasis by targeting specific mRNAs [27]. For example, miR-125 inhibits the proliferation and invasion of CRC by targeting PDZ-binding…”

Section: Discussionmentioning

confidence: 99%

miRNA-6715-5p Inhibits Cellular Proliferation and Invasion in Colorectal Cancer by Directly Targeting CST4

Ding

Zhou

Zhang

2021

Journal of Oncology

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Background. Data on the correlation between CST4 and colorectal cancer (CRC) metastasis are scarce. The aim of this study was to analyze CST4 expression and investigate its biological roles and related microRNA- (miRNA-) mediated regulation in CRC. Methods. The expression of CST4 was examined in cancer tissues and their corresponding adjacent normal tissues from 40 gastric adenocarcinoma patients. The expression level of CST4 in specimens (cancer and normal tissues) was assessed through immunohistochemistry and/or quantitative polymerase chain reaction. miRNAs targeting CST4 in CRC were predicted by bioinformatics software. CST4 was knocked down in HCT116 cells and candidate miRNAs were transfected into HCT116 cells, and the effects of CST4 knockdown and miRNA transfection on cell proliferation and invasion were examined using CCK8, cell colony formation, and Transwell migration assays. Luciferase double-reporter assays were performed to verify the relationship between miRNA and CST4. Results. The expression of CST4 in CRC tissues was significantly higher than that in normal paracancerous tissues, but the results for miRNA-6715-5p were opposite. Regardless of CST4 knockdown or miRNA-6715-5p overexpression, the proliferation and invasion ability of HCT116 cells decreased significantly. Luciferase double-reporter assays showed that the upregulation of miR-6715-5p significantly reduced the luciferase activities of the CST4 3′-UTR plasmid in HCT116 cells. Conclusion. CST4 may be involved in CRC proliferation and metastasis. miRNA-6715-5p directly targets CST4 and negatively regulates its expression.

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Section: Discussionmentioning

confidence: 99%

miRNA-6715-5p Inhibits Cellular Proliferation and Invasion in Colorectal Cancer by Directly Targeting CST4

Ding

Zhou

Zhang

2021

Journal of Oncology

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“…[ 19 ] It was noticeable to find that miR-367 is downregulated in individuals with colorectal cancer, and this downregulation may be responsible for the poor survival rate of these patients. [ 20 ] Furthermore, when highly expressed miR-367-3p is combined with its target gene and other tumor suppressors to form a positive feedback loop in glioma microenvironment, neoplasm is repressed and survival rate is improved. [ 21 ] In this study, according to the results of RT-qPCR, miR-367 expression in serum of BC patients is lower than that in the normal subjects.…”

Section: Discussionmentioning

confidence: 99%

Correlation of microRNA-367 in the clinicopathologic features and prognosis of breast cancer patients

Liu¹,

Pan²,

Fu³

2021

Medicine

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Breast cancer (BC) is a malignant tumor originating from cells of the breast. Notably, microRNAs have been recognized as biomarkers of BC metastasis. The present study is designed to evaluate the association between microRNA (miR)-367 expression and BC with the variance of clinicopathologic features and prognosis. Initially, 63 BC patients were allocated in the BC group, while the other 40 healthy volunteers were recruited as the control group. miR-367 expression in the serum of patients and healthy controls was detected using real-time polymerase chain reaction. Furthermore, the relation between miR-367 in serum and clinicopathologic features and prognosis of BC patients was accessed. miR-367 expression in serum of the BC group was evidently lower than that in the control group (all P < .001). Besides, miR-367 underexpression in the BC group was closely associated with the variance in tumor nodes metastasis advanced stage, tumor diameter, and lymph node metastasis of BC (all P < .001). In addition, compared with the control group, poorly expressed miR-367 BC group had short period of disease-free survival and overall survival (all P < .001). Our study demonstrated that miR-367 expression is associated with BC clinicopathologic features and prognosis. This investigation may offer new insight for BC treatment.

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“…However, opposing functions of individual members of the aforementioned polycistron, miR-99a/100 [ 61 ] and LET-7 [ 62 ] were also identified. Therefore, understanding how clusters (and their individual miRNAs) function is important as they may prove useful in therapeutic targeting [ 63 ]. Altogether, given the functional variability of individual miRNAs and clusters, it is of no surprise that dysregulation can result in different disease types.…”

Section: Mirna Clusters and Their Role In Tumor Developmentmentioning

confidence: 99%

The Role of miRNAs, miRNA Clusters, and isomiRs in Development of Cancer Stem Cell Populations in Colorectal Cancer

Stark

Facey²,

Viswanathan

et al. 2021

IJMS

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MicroRNAs (miRNAs or miRs) have a critical role in regulating stem cells (SCs) during development and altered expression can cause developmental defects and/or disease. Indeed, aberrant miRNA expression leads to wide-spread transcriptional dysregulation which has been linked to many cancers. Mounting evidence also indicates a role for miRNAs in the development of the cancer SC (CSC) phenotype. Our goal herein is to provide a review of: (i) current research on miRNAs and their targets in colorectal cancer (CRC), and (ii) miRNAs that are differentially expressed in colon CSCs. MicroRNAs can work in clusters or alone when targeting different SC genes to influence CSC phenotype. Accordingly, we discuss the specific miRNA cluster classifications and isomiRs that are predicted to target the ALDH1, CD166, BMI1, LRIG1, and LGR5 SC genes. miR-23b and miR-92A are of particular interest because our previously reported studies on miRNA expression in isolated normal versus malignant human colonic SCs showed that miR-23b and miR-92a are regulators of the LGR5 and LRIG1 SC genes, respectively. We also identify additional miRNAs whose expression inversely correlated with mRNA levels of their target genes and associated with CRC patient survival. Altogether, our deliberation on miRNAs, their clusters, and isomiRs in regulation of SC genes could provide insight into how dysregulation of miRNAs leads to the emergence of different CSC populations and SC overpopulation in CRC.

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miRNA Clusters with Down-Regulated Expression in Human Colorectal Cancer and Their Regulation

Cited by 51 publications

References 176 publications

miRNA-6715-5p Inhibits Cellular Proliferation and Invasion in Colorectal Cancer by Directly Targeting CST4

miRNA-6715-5p Inhibits Cellular Proliferation and Invasion in Colorectal Cancer by Directly Targeting CST4

Correlation of microRNA-367 in the clinicopathologic features and prognosis of breast cancer patients

The Role of miRNAs, miRNA Clusters, and isomiRs in Development of Cancer Stem Cell Populations in Colorectal Cancer

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