MiRNA-29a regulates the expression of numerous proteins and reduces the invasiveness and proliferation of human carcinoma cell lines

Muniyappa, Mohan Kumar; Dowling, Paul; Henry, Michael; Meleady, Paula; Doolan, Padraig; Gammell, Patrick; Clynes, Martin; Barron, Niall

doi:10.1016/j.ejca.2009.09.014

Cited by 108 publications

(72 citation statements)

References 33 publications

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“…The three miR-29 isoforms are arranged in two clusters: miR-29b1/miR-29a located on chromosome 7q32 and miR-29b2/miR-29c located on chromosome 1q32. miR-29a has been shown to reduce invasiveness and proliferation of human carcinoma cell lines (Muniyappa et al, 2009). miR-29b was previously correlated with good prognosis in patients with acute myeloid leukemia, and has a tumor suppressor function in leukemic blasts by targeting apoptosis, cell cycle and proliferation pathways (Garzon et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-29b is involved in the Src-ID1 signaling pathway and is dysregulated in human lung adenocarcinoma

et al. 2012

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Section: Discussionmentioning

confidence: 99%

MicroRNA-29b is involved in the Src-ID1 signaling pathway and is dysregulated in human lung adenocarcinoma

et al. 2012

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“…Downregulation of miR-29a expression has been found in colorectal, gastric, and lung cancers, acute myeloid leukemia, and lymphoma. 13,[15][16][17][18] Furthermore, a significant downregulation TRIM68 is a target of miR-29a and miR-1256 and was upregulated in PCa. By comparing computerized prediction of miRNA targets with mRNA microarray analysis, we found that TRIM68 could be a target of both miR-29a and miR-1256.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, both PGK-1 and AR genes are located within Xqll-Xql3 region and PGK-1 short tandem repeat polymorphism linkage to AR in the expression of miR-29a has been found to be correlated with short survival, more invasive phenotype, and early recurrence. [16][17][18] Therefore, miR-29a is believed to play important roles in the inhibition of cancer development and progression and the loss of its expression is in part responsible for tumor development and progression. However, there is no report on the expression level of miR-29a in PCa cells, normal prostate epithelial cells, human PCa specimens or normal human prostate tissues.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic deregulation of miR-29a and miR-1256 by isoflavone contributes to the inhibition of prostate cancer cell growth and invasion

et al. 2012

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“…Five of these miRNA have been reported to be oncogenic and two of the miRNA are believed to have tumor-suppressive functions (Table 1). (15,(17)(18)(19)(20)(21)(22)(23)(24) Compartment-specific miRNA overexpression was not detected in the liver invasion front with respect to the same miRNA in the liver, the tumor invasion front or the tumor center. Similarly, expression analysis of samples taken from the tumor invasion front was compared with samples taken from pure liver, the liver invasion front or the tumor center.…”

Section: Resultsmentioning

confidence: 98%

Invasion front‐specific expression and prognostic significance of microRNA in colorectal liver metastases

Kahlert¹,

Klupp²,

Brand

et al. 2011

Cancer Science

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The tumor edge of colorectal cancer and its adjacent peritumoral tissue is characterized by an invasion front-specific expression of genes that contribute to angiogenesis or epithelial-to-mesenchymal transition. Dysregulation of these genes has a strong impact on the invasion behavior of tumor cells. However, the invasion front-specific expression of microRNA (miRNA) still remains unclear. Therefore, the aim of the present study was to investigate miRNA expression patterns at the invasion front of colorectal liver metastases. Laser microdissection of colorectal liver metastases was performed to obtain separate tissue compartments from the tumor center, tumor invasion front, liver invasion front and pure liver parenchyma. Microarray expression analysis revealed 23 miR-NA downregulated in samples from the tumor invasion front with respect to the same miRNA in the liver, the liver invasion front or the tumor center. By comparing samples from the liver invasion front with samples from pure liver parenchyma, the tumor invasion front and the tumor center, 13 miRNA were downregulated. By quantitative RT-PCR, we validated the liver invasion front-specific downregulation of miR-19b, miR-194, let-7b and miR-1275 and the tumor invasion front-specific downregulation of miR-143, miR-145, let-7b and miR-638. Univariate analysis demonstrated that enhanced expression of miR-19b and miR-194 at the liver invasion front, and decreased expression of let-7 at the tumor invasion front, is an adverse prognostic marker of tumor recurrence and overall survival. In conclusion, the present study suggests that invasion front-specific downregulation of miRNA in colorectal liver metastases plays a pivotal role in tumor progression. (Cancer Sci 2011; 102: 1799-1807 C olorectal cancer is the third most frequent cancer in Western countries.(1) Approximately 50% of patients with colorectal cancer die from distant metastases, and in particular liver metastases, within 10 years of the initial diagnosis.(2) Recent studies have demonstrated that microRNA (miRNA) play important roles in tumor invasion and metastasis.(3-7) miRNA are small, non-coding RNA that silence specific target genes in mammalian cells by repressing translation.(8) Depending on the target gene, miRNA can act as either oncogenes or tumor suppressive genes.(9) These findings are in agreement with clinical studies, which have shown that the aberrant expression of specific miRNA can be used as powerful prognostic and predictive markers in colorectal cancer.(10,11) Because current prognostic markers of many cancers, including colorectal liver metastases, poorly predict metastatic progression or tumor response to chemotherapy, the identification of such prognostic markers is the subject of intense research.However, most reports in the literature on the use of miRNA as prognostic markers investigated only macroscopically identifiable tumor tissues and did not address whether the relevant miRNA was mainly expressed in tumor epithelial cells or in tumor-associated stromal cells. Furthe...

show abstract

MiRNA-29a regulates the expression of numerous proteins and reduces the invasiveness and proliferation of human carcinoma cell lines

Cited by 108 publications

References 33 publications

MicroRNA-29b is involved in the Src-ID1 signaling pathway and is dysregulated in human lung adenocarcinoma

MicroRNA-29b is involved in the Src-ID1 signaling pathway and is dysregulated in human lung adenocarcinoma

Epigenetic deregulation of miR-29a and miR-1256 by isoflavone contributes to the inhibition of prostate cancer cell growth and invasion

Invasion front‐specific expression and prognostic significance of microRNA in colorectal liver metastases

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