miRNA-21 as High Potential Prostate Cancer Biomarker in Prostate Cancer Patients in Indonesia

Gunawan, Regina R; Astuti, Indwiani; Danarto, H R

doi:10.31557/apjcp.2023.24.3.1095

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…Gunawan и соавт. на выборке из 20 пациентов с РПЖ и 20 пациентов с ДГПЖ экспрессия miR-21-5p в образцах мочи различалась более чем в 12 раз между группами и продемонстрировала более высокие чувствительность и специфичность по сравнению с ПСА [48]. Эти данные в совокупности с результатами представленного исследования могут свидетельствовать о потенциальной диагностической значимости определения miR-21 у пациентов с РПЖ.…”

Section: диагностика и лечение опухолей мочеполовой системы рак предс...unclassified

Assessment of miR-21-5p, miR-451a, and miR-144-3p level in urine in differential diagnosis of localized prostate cancer

Dolotkazin,

Averinskaya,

Knyazev

et al. 2024

Onkourologiâ

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Background. Limited sensitivity and specificity of existing prostate cancer (PCa) diagnosis methods drive the search for new markers. A number of studies has demonstrated the potential for measuring expression of certain microRNAs in urine.Aim. To evaluate the diagnostic potential of measuring microRNA expression in urine in PCa.Materials and methods. A collection of urine sediment samples from 19 patients with benign prostatic hyperplasia and 44 patients with PCa was analyzed. RNA was isolated using the miRNEasy Serum/Plasma Kit. 16 µL of RNA isolated from each sample were converted into cDNA, which served as a template for real-time polymerase chain reaction. For sequencing, microRNA libraries were prepared using MGIEasy Small RNA Library Prep Kit v.2.0. The formed DNA nanoballs were placed into an MGI DNBSEQ-G400 sequencer. Sequencing results were processed using IsoMiRmap. Differences in microRNA abundance were analyzed using DESeq2. For miRNA-21, high-throughput sequencing data were corroborated by the results of quantitative real-time polymerase chain reaction.Results. 1154 types of microRNA were identified, 11 were differentially represented in all comparison groups. The most significant differences in cell sediment between benign prostatic hyperplasia and PCa patients were recorded for miR-451a (area under the curve (AUC) 0.98). Additionally, the abundance levels of two microRNA isoforms were significantly different: hsa-miR-144-3p|-1 (AUC 0.96) and hsa-miR-21-5p|+4 (AUC 0.68).Сonclusion. This study confirms that altered expression of microRNAs miR-21, miR-451a and miR-144-3p is associated with PCa, can be detected in urine samples, and can also be a potential non-invasive diagnostic criterion.

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Section: диагностика и лечение опухолей мочеполовой системы рак предс...unclassified

Assessment of miR-21-5p, miR-451a, and miR-144-3p level in urine in differential diagnosis of localized prostate cancer

Dolotkazin,

Averinskaya,

Knyazev

et al. 2024

Onkourologiâ

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MicroRNA´S COMO BIOMARCADORES DO CÂNCER DE PRÓSTATA: UMA REVISÃO INTEGRATIVA

Telis,

Pimenta,

Teixeira

et al. 2023

Rev. Foco

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MicroRNA é um constituinte do genoma não-codificante capaz de regular a síntese proteica em nível pós-transcricional, silenciando ou induzindo a degradação de RNA mensageiros. A expressão de microRNA é basal em todos os tipos celulares, ao passo que o perfil de expressão é particular a cada um. O conjunto de microRNAs produzidos em uma célula se torna uma característica exclusiva a ela, de acordo com suas próprias funções metabólicas. Investigações dos mecanismos moleculares do câncer revelaram que o miRNA desempenha um papel importante nesta doença, estando pontualmente desregulado e tendo uma participação direta na mediação de processos de invasão tecidual e divisão celular. A presente revisão investigou os microRNAs no câncer de próstata (CaP) a partir de trabalhos da literatura, com o intuito de formar um painel de biomarcadores para o diagnóstico mais precoce da doença. Trata-se de um estudo do tipo revisão integrativa na qual foram utilizadas as bases de dados: U.S National Library of Medicine (PUBMED), Scientific Eletronic Library Online (SciELO) e Google Acadêmico. Os descritores utilizados foram “Prostatic Neoplasms” e “microRNA”, associados ao operador boleano AND, visando a resposta da seguinte pergunta norteadora: Quais microRNA´s são potenciais biomarcadores do CaP? Notou-se que a heterogeneidade do tumor pode estar atrelada a falta de evidências para a maioria dos microRNAs investigados e que o microRNA (miR-21) apresenta potencial para biomarcador do CaP.

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miRNA-21 as High Potential Prostate Cancer Biomarker in Prostate Cancer Patients in Indonesia

Cited by 2 publications

References 17 publications

Assessment of miR-21-5p, miR-451a, and miR-144-3p level in urine in differential diagnosis of localized prostate cancer

Assessment of miR-21-5p, miR-451a, and miR-144-3p level in urine in differential diagnosis of localized prostate cancer

MicroRNA´S COMO BIOMARCADORES DO CÂNCER DE PRÓSTATA: UMA REVISÃO INTEGRATIVA

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