MiRNA-203 suppresses cell proliferation, migration and invasion in colorectal cancer via targeting of EIF5A2

Deng, Bingbing; Wang, Bin; Fang, Jie; Zhu, Xiaoye; Cao, Zhongwei; Qi, Lin; Zhou, Lisheng; Sun, Xiaojiang

doi:10.1038/srep28301

Cited by 90 publications

(62 citation statements)

References 37 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNAs (miRNAs) are a subset of non-coding RNAs of ~19-23 nucleotides in length, which post-transcriptionally regulate gene expression (5). miRNAs play a role in crucial biological processes including proliferation (6,7), differentiation (8), chemosensitivity (9,10) and apoptosis (11,12). Studies have shown that aberrations in the expression of certain miRNAs may cause or contribute to human diseases, including cancer (13).…”

Section: Introductionmentioning

confidence: 99%

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Othman

Nagoor

2017

International Journal of Oncology

View full text Add to dashboard Cite

Lung cancer remains a major health problem with a low 5-year survival rate of patients. Recent studies have shown that dysregulation of microRNAs (miRNAs) are prevalent in lung cancer and these aberrations play a significant role in the progression of tumour progression. In the present study, bioinformatics analyses was employed to predict potential miR-608 targets, which are associated with signaling pathways involved in cancer. Luciferase reporter assay identified AKT2 as a novel target of miR-608, and suppression of its protein levels was validated through western blot analysis. Zebrafish embryos were microinjected with cells transfected with miR-608 to elucidate the role of miR-608 in vivo, and immunostained with antibodies to detect activated caspase-3. We present the first evidence that miR-608 behaves as a tumour suppressor in A549 and SK-LU-1 cells through the regulation of AKT2, suggesting that selective targeting of AKT2 via miR-608 may be developed as a potential therapeutic strategy for miRNA-based non-small cell lung cancer (NSCLC) therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Othman

Nagoor

2017

International Journal of Oncology

View full text Add to dashboard Cite

show abstract

“…Furthermore, elevated expression of WHSC1 is often observed in many types of human cancers, and expression product of WHSC1 is essential for the growth of cancer cells [52][53][54] . The gene EIF5A2 was related to not only ESCA [55][56][57] , but also breast cancer 58 , lung cancer 59,60 , bladder cancer 61,62 , stomach cancer [63][64][65] , oral cancer 66 , liver cancer 67 , and colorectal cancer 68 . By checking the expression of these genes detected in tumors (RNA-seq data in TCGA), most of these genes are significantly up-regulated in tumors ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Finding new cancer epigenetic and genetic biomarkers from cell-free DNA by combining SALP-seq and machine learning: esophageal cancer as an example

Liu

Xia

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Cancer is an important public health problem worldwide and its early diagnosis and effective prognosis are critical for its treatment. In recent years, as a good material for cancer liquid biopsy, plasma cell-free DNA (cfDNA) has been widely analyzed by next generation sequencing (NGS) for finding new molecular markers for cancer diagnosis such as size, methylation and end coordinate. However, the current studies did not still involve esophageal cancer (ESCA), a main cancer that seriously threatens human health and life in China. Here we therefore tried to find new biomarkers for this cancer from cfDNA. Materials & methods: Thirty cfDNA samples from 26 ESCA patients and 4 healthy people were used to construct the NGS libraries and sequenced by using SALP-seq. The sequencing data were analyzed with variant bioinformatics methods for finding ESCA molecular biomarkers. Results & conclusion: We identified 103 epigenetic markers (including 54 genome-wide and 49 promoter markers) and 37 genetic markers for ESCA. These markers provide new molecular biomarkers for ESCA diagnosis, prognosis and therapy. Importantly, this study provides a new pipeline for finding new molecular markers for cancers from cfDNA by combining SALP-seq and machine learning. Finally, by finding new molecular markers for ESCA from cfDNA, this study sheds important new insights on the clinical worth of cfDNA. 2 the incidence of cancer has not changed significantly before, cancer mortality has continued to decline, not only because of the development of medical standards, but also for preventive screening.Tissue biopsy is still the gold standard for diagnosing tumors, but due to the traumatic nature of patients, it brings a lot of interference to the dynamic treatment of patients. There are also many risks and ethical issues in tissue biopsy, which makes it have certain limitations. Liquid biopsy is a kind of cutting-edge technology to analyze a range of tumor material in the blood or other body fluids in a minimally invasive or noninvasive manner. The tumor material includes circulating tumor cells (CTCs), cell-free tumor DNA (ctDNA), messenger RNA (mRNA), microRNA (miRNA), and exosomes. Of these tumor biomarkers, ctDNA are most widely recognized in clinical application 4 . Comparing to ctDNA, cell-free DNA (cfDNA) is a broader term which describes DNA that is freely circulating, but is not necessarily of tumor origin. CtDNA refers to the cfDNA from tumor.CfDNA in human body fluids, such as plasma, was discovered in 1948 5 . Most of the plasma cfDNA originated from the hematopoietic system in healthy subjects, but in clinical patients (e.g. pregnancy and cancer), the related cells/tissues would release additional DNA into the plasma 6,7 . The detection of this perturbation would allow us to diagnose the abnormality for people in a noninvasive way. In recent years, methods based on the analysis of plasma cfDNA which is as an emerging technology have been largely explored for noninvasive prenatal testing (NIPT) and cancer liquid biopsy 8,9...

show abstract

“…The primers used to amply the 3′‐UTR of SOX2 were designed and synthesized by Sangong (Shanghai, China). The mutagenesis of the 3′‐UTR segment of SOX2 was performed as previously described . Both wild‐type and mutant luciferase reporter constructs were confirmed by sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…The mutagenesis of the 3′-UTR segment of SOX2 was performed as previously described. 34 Both wild-type and mutant luciferase reporter constructs were confirmed by sequencing. GBM cells were transiently cotransfected with 0.2 μg of pGL3-SOX2-3′-UTR or pGL3-SOX2-3′-UTR Mut, 0.02 μg of pRL-TK-Renilla luciferase reporter plasmids (Promega) containing the Renilla luciferase for normalization and with 5 pmol of miR-429 mimic or negative control.…”

Section: Bioinformatics and Luciferase Reporter Assaymentioning

confidence: 99%

MiR‐429 suppresses glioblastoma multiforme by targeting SOX2

Dong

Hao

Cui

et al. 2017

Cell Biochemistry & Function

View full text Add to dashboard Cite

Accumulating evidence has shown that miR-429 plays an important role in the development and progression of tumour. However, the role of miR-429 in glioblastoma multiforme (GBM) remains largely unknown. The present study is designed to investigate the function of miR-429 in GBM and to explore the molecular mechanism underlying its function. The expression level of miR-429 was detected in GBM tissues and cell lines by quantitative real-time polymerase chain reaction. The effect of overexpression of miR-429 on in vitro cell proliferation, apoptosis and invasion was examined. Western blot analysis was used to detect the influence of miR-429 on the expression of target gene, and Pearson analysis was used to calculate the correlation between the expression of targets gene and the miR-429 in GBM tissues. Our study shows that miR-429 is downregulated in GBM tissues compared with noncancerous tissues (P < .01). In addition, the expression of miR-429 in GBM cell lines is also significantly lower (P < .01). Enforced expression of miR-429 inhibits GBM cells proliferation, induces apoptosis and suppresses invasion and leads to the downregulation of the SOX2 protein. Moreover, the expression level of miR-429 in GBM tissues shows inverse relationship with the expression level of SOX2 protein. Our findings suggest that miR-429 represents a potential tumour-suppressive miRNA and plays an important role in GBM progression by directly targeting SOX2.

show abstract

MiRNA-203 suppresses cell proliferation, migration and invasion in colorectal cancer via targeting of EIF5A2

Cited by 90 publications

References 37 publications

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Finding new cancer epigenetic and genetic biomarkers from cell-free DNA by combining SALP-seq and machine learning: esophageal cancer as an example

MiR‐429 suppresses glioblastoma multiforme by targeting SOX2

Contact Info

Product

Resources

About