MiRNA-181a Regulates Adipogenesis by Targeting Tumor Necrosis Factor-α (TNF-α) in the Porcine Model

Li, Hongyi; Chen, Xiao; Guan, Lan; Qi, Qien; Shu, Gang; Jiang, Qingyan; Li, Yuan; Xi, Qianyun; Zhang, Yongliang

doi:10.1371/journal.pone.0071568

Cited by 101 publications

(82 citation statements)

References 54 publications

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“…Therefore, pigs are ideal animal models to study human diseases related to adipose [13]. Recently, researchers have studied the mechanism of obesity using pigs and made some progress [35]. In this study, the Laiwu pig and Large White pig, with significant differences in adipopexis, were chosen as the animal models.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

Zhang¹,

Huang²,

Guo³

et al. 2017

Oncotarget

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The domestic pig, an important species in the animal production industry, is also a model system for studying fat deposition and fat-associated diseases in humans. In order to investigate the genetic relationships of the miRNAs that may regulate fat deposition, we performed a genome-wide analysis of miRNAs derived from subcutaneous adipose tissue of Laiwu and Large White pig using RNA-seq. A total of 26,486,906 reads were obtained. Further analysis indicated that 39 known miRNAs and 56 novel miRNAs had significantly differential expression between the two breeds of pigs. Gene Ontology and KEGG pathway analysis revealed that the predicted targets of these differentially expressed miRNAs were involved in several fat-associated pathways, such as the PPAR, MAPK and Wnt signaling pathways. In addition, we found that three miRNAs, ssc-miR-133a-3p, ssc-miR-486 and ssc-miR-1, had an impact on the development of porcine subcutaneous fat through the PPAR signaling pathway. This study provides clues to understand the potential mechanisms of adipogenesis and fat deposition between two different pig breeds. Furthermore, these results may also contribute to the research involving human obesity.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

Zhang¹,

Huang²,

Guo³

et al. 2017

Oncotarget

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“…47,48 The TLR/NFκB-related miR-181a decreased in monocytes of obese individuals and is a putative biomarker of metabolic syndrome. 49 Moreover, miR-181a could promote adipogenesis via targeting TNFα, 50 and downregulation of miR-181a could improve hepatic insulin sensitivity by upregulating sirtuin-1. 51 In this study, miR-181d was predicted to be a putative regulator of Adamts1.…”

Section: Discussionmentioning

confidence: 99%

The miR-181d-regulated metalloproteinase Adamts1 enzymatically impairs adipogenesis via ECM remodeling

Chen

Ning

et al. 2016

Cell Death Differ

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The extracellular matrix (ECM) maintenance is crucial to the structural integrity of adipocytes and whole adipose tissue formation. However, the potential impact of the ECM on adipocyte lineage commitment is unclear. Herein, we demonstrate that forced expression of matrix-associated metalloproteinase Adamts1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), which we show is targeted by microRNA-181d (miR-181d) during BMP4-induced adipocytic lineage commitment, markedly impairs adipocyte commitment. Conversely, siRNA-induced inhibition of Adamts1 promotes adipocyte commitment. Adamst1 metalloprotease activity is required for this inhibition and is determined to function via remodeling ECM components followed by activating FAK-ERK signaling pathway during the commitment process. Furthermore, ablation of Adamts1 in adipose tissue increases adipose tissue mass, reduces insulin sensitivity, and disrupts lipid homeostasis. This finding is consistent with Adamts1 decreased expression in the adipose tissue of obese mice and an inverse correlation of Adamts1 expression with body mass index in humans. Collectively, our results indicate that Adamts1 acts as an ECM 'modifier', with miR-181d-induced downregulation, that regulates adipocyte lineage commitment and obesity.

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“…74 In RO-treated P20 cells, miR-138 was up-regulated up twofold ( Among miRNAs involved in adipogenesis regulation, miR-181a were down-expressed in RO-treated cells and this is in agreement with results achieved in subcutaneous fat tissue of seven-day-old piglets. 75 In those cells, the suppression of miR-181a led to inhibition of adipocyte differentiation by affecting the expression of tumor necrosis factor-a, as well as genes involved in adipogenesis. 75 In 3T3-L1 cells, miR-181a was also included among miRNAs that target transcription factors or differentiation markers during adipogenesis and that activate Wnt signaling.…”

mentioning

confidence: 99%

Effects of Rosmarinus officinalis extract on human primary omental preadipocytes and adipocytes

Stefanon

Pomari

Colitti

2015

Exp Biol Med (Maywood)

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The prevalence of obesity is increasing all over the world. Although it has been shown that natural substances influence fat metabolism, little is known about the effect on cellular and molecular mechanisms in human. In this in vitro study, the activity of Rosmarinus officinalis (RO) standardized extract in modulating human primary visceral preadipocytes differentiation, lipolysis, and apoptosis was investigated. Moreover, gene expression of key adipogenesis modulators and microRNAs-seq were evaluated. Preadipocytes treated with RO extract significantly reduced triglyceride incorporation during maturation in a dose-dependent manner without affecting cell viability. In addition, RO extract stimulated lipolytic activity in differentiating preadipocytes and mature adipocytes in treated cells compared to controls. Differentiating preadipocytes incubated in the presence of RO extract showed a decreased expression of cell cycle genes such as cyclin D1, cyclin-dependent kinase 4, cyclin-dependent kinase inhibitor 1A (p21, Cip1) and an increased expression of GATA binding protein 3, wingless-type MMTV integration site family, member 3A mRNA levels. Recent studies have demonstrated that some phytochemicals alter the expression of specific genes and microRNAs that play a fundamental role in the pathogenesis of obesity and related diseases. Interestingly, genes modulated in RO-treated cells were found to be validated miRNAs targets, such as let-7f-1, miR-17, and miR-143. The results indicated that RO extract modulates human adipocyte differentiation and significantly interferes with adipogenesis and lipid metabolism, supporting its interest as dietary supplement.

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MiRNA-181a Regulates Adipogenesis by Targeting Tumor Necrosis Factor-α (TNF-α) in the Porcine Model

Cited by 101 publications

References 54 publications

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

The miR-181d-regulated metalloproteinase Adamts1 enzymatically impairs adipogenesis via ECM remodeling

Effects of Rosmarinus officinalis extract on human primary omental preadipocytes and adipocytes

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