miRNA-17 Members that Target<i>Bmpr2</i>Influence Signaling Mechanisms Important for Embryonic Stem Cell Differentiation In Vitro and Gastrulation in Embryos

Larabee, Shannon M.; Coia, H; Jones, Shiloh B.; Cheung, E.; Gi, Gallicano

doi:10.1089/scd.2014.0051

Cited by 25 publications

(17 citation statements)

References 87 publications

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“…It was demonstrated that hsa‐let‐7b‐5p suppressed the osteogenic differentiation capacity of stem cells (Wang et al, ). hsa‐miR‐17‐3p also influenced bone metabolism through the suppression of BMPR2 (Larabee, Coia, Jones, Cheung, & Gallicano, ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

et al. 2018

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Objectives MicroRNAs (miRNAs) may play an important role in inflammatory response. However, the involvement of miRNAs in the pathogenesis of periodontitis is unclear. The present study aimed to compare the miRNA expression profiles in individuals with chronic (CP) or aggressive (AP) periodontitis. Materials and methods Eighteen non‐smoker individuals (CP = 9 and AP = 9) without any history of systemic diseases or previous periodontal therapies were selected at the Clinics of Periodontology from the Federal University of Minas Gerais. Gingival tissue samples were collected during the initial periodontal therapy. miRNAs were isolated, and expression patterns of 754 miRNAs were assessed with a quantitative miRNA PCR array. miRNAs expression profiles were compared between CP and AP groups. Results There were no differences observed in the miRNAs expression profiles between CP and AP (p > 0.05). According to the microarray analyses, the most expressed miRNAs in both groups were hsa‐miR‐1274b, hsa‐let‐7b‐5p, hsa‐miR‐24‐3p, hsa‐miR‐19b‐3p, hsa‐miR‐720, hsa‐miR‐126‐3p, hsa‐miR‐17‐3p and hsa‐miR‐21‐3p. Conclusion Findings suggested no differences in miRNAs expression profiles between chronic and aggressive forms of periodontitis. The overexpression of specific miRNAs could provide insights into the pathogenesis of both forms of the disease.

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Section: Discussionmentioning

confidence: 99%

Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

et al. 2018

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“…miRNAs are non-coding RNAs that regulate many biological processes, including embryonic development [39–42]. miRNAs play an important role in the maternal-conceptus interface[43].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Fatty Acid Synthase Reduces Blastocyst Hatching through Regulation of the AKT Pathway in Pigs

2017

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Fatty acid synthase (FASN) is an enzyme responsible for the de novo synthesis of long-chain fatty acids. During oncogenesis, FASN plays a role in growth and survival rather than acting within the energy storage pathways. Here, the function of FASN during early embryonic development was studied using its specific inhibitor, C75. We found that the presence of the inhibitor reduced blastocyst hatching. FASN inhibition decreased Cpt1 expression, leading to a reduction in mitochondria numbers and ATP content. This inhibition of FASN resulted in the down-regulation of the AKT pathway, thereby triggering apoptosis through the activation of the p53 pathway. Activation of the apoptotic pathway also leads to increased accumulation of reactive oxygen species and autophagy. In addition, the FASN inhibitor impaired cell proliferation, a parameter of blastocyst quality for outgrowth. The level of OCT4, an important factor in embryonic development, decreased after treatment with the FASN inhibitor. These results show that FASN exerts an effect on early embryonic development by regulating both fatty acid oxidation and the AKT pathway in pigs.

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“…In general, one gene can be repressed by multiple miRNAs and one miRNA may repress multiple target genes, which results in the establishment of complex regulatory feedback loops . In multiple cellular processes, including development, proliferation, and differentiation, miRNAs fine‐tune cellular fate by targeting important transcription factors or key pathways .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-145 Regulates Neural Stem Cell Differentiation Through the Sox2–Lin28/let-7 Signaling Pathway

2016

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MicroRNAs (miRNAs or miRs) regulate several biological functions, including cell fate determination and differentiation. Although miR-145 has already been described to regulate glioma development, its precise role in neurogenesis has never been addressed. miR-145 represses sexdetermining region Y-box 2 (Sox2), a core transcription factor of embryonic stem cells (ESCs), to inhibit pluripotency and self-renewal in human ESCs. In addition, the Sox2-Lin28/let-7 signaling pathway regulates proliferation and neurogenesis of neural precursors. In this study, we aimed to investigate the precise role of miR-145 in neural stem cell (NSC) fate decision, and the possible involvement of the Sox2-Lin28/let-7 signaling pathway in miR-145 regulatory network. Our results show for the first time that miR-145 expression significantly increased after induction of mouse NSC differentiation, remaining elevated throughout this process. Forced miR-145 downregulation decreased neuronal markers, namely bIII-tubulin, NeuN, and MAP2. Interestingly, throughout NSC differentiation, protein levels of Sox2 and Lin28, a well-known suppressor of let-7 biogenesis, decreased. Of note, neuronal differentiation also resulted in let-7a and let-7b upregulation. Transfection of NSCs with anti-miR-145, in turn, increased both Sox2 and Lin28 protein levels, while decreasing both let-7a and let-7b. More importantly, Sox2 and Lin28 silencing partially rescued the impairment of neuronal differentiation induced by miR-145 downregulation. In conclusion, our results demonstrate a novel role for miR-145 during NSC differentiation, where miR-145 modulation of Sox2-Lin28/let-7 network is crucial for neurogenesis progression. STEM CELLS 2016;34:1386-1395 SIGNIFICANCE STATEMENTNeural stem cells (NSCs) are a potential source of repairing cells. Although neurogenesis occurs in adult mammalian brain, NSCs produce very few neurons during aging or after injury. Thus, the identification of novel players that improve neural plasticity can have a huge impact in age-associated neurogenesis and cognitive decline. Recent evidence suggests that microRNAs (miRNAs or miRs), small noncoding RNA molecules that regulate gene expression, affect NSC fate. However, the underlying molecular mechanisms driving the enhancement of neuroplasticity by miRNAs remain largely unknown. Using in vitro models of mouse NSC, we revealed the molecular mechanisms by which miR-145 drives NSCs toward neuronal differentiation. The results from our study might add significant new information on key players responsible for neuroplasticity enhancement, thus contributing to improve the health and life quality of the aging population.

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miRNA-17 Members that TargetBmpr2Influence Signaling Mechanisms Important for Embryonic Stem Cell Differentiation In Vitro and Gastrulation in Embryos

Cited by 25 publications

References 87 publications

Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

Inhibition of Fatty Acid Synthase Reduces Blastocyst Hatching through Regulation of the AKT Pathway in Pigs

MicroRNA-145 Regulates Neural Stem Cell Differentiation Through the Sox2–Lin28/let-7 Signaling Pathway

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