miRNA-15a/16: As Tumor Suppressors and More

Huang, Enyu; Liu, Ronghua; Chu, Yiwei

doi:10.2217/fon.15.101

Cited by 75 publications

(68 citation statements)

References 65 publications

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“…The function of miR-16 has been primarily investigated in the field of oncology, and has been demonstrated to act as a tumor suppressor, an oncomiR (an miRNA associated with cancer), a modulator of the immune response and a negative regulator of angiogenesis (18). It has also been reported that miR-16 promotes α-synuclein aggregation by downregulating heat shock protein 70 (HSP70) in a neuroblastoma cell line (19).…”

Section: Discussionmentioning

confidence: 99%

Serum microRNA expression profiling in patients with multiple system atrophy

Kume

Iwama

Deguchi

et al. 2017

Journal of the Neurological Sciences

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Section: Discussionmentioning

confidence: 99%

Serum microRNA expression profiling in patients with multiple system atrophy

Kume

Iwama

Deguchi

et al. 2017

Journal of the Neurological Sciences

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“…In oncological studies, a growing number of miRNAs have been confirmed to have an association with a large number of neoplasms (9–11). For example, miR-15a and miR-16 may be closely associated with cancer pathogenesis (12,13). miR-143, as a tumor suppressor, inhibits the growth, and induces apoptosis of, gastric cancer cells through targeting cyclooxygenase-2 (14).…”

Section: Introductionmentioning

confidence: 99%

The diagnostic effect of serum miR-196b as biomarker in colorectal cancer

2016

Biomedical Reports

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The microRNA, miR-196b, serves a role in normal cell differentiation, proliferation and tumorigenesis of different types of cancer. The aim of the present study was to explore the serum expression of miR-196b in colorectal cancer (CRC) and its correlation with clinicopathological features. Sera samples were obtained from 103 patients with CRC, 51 patients with colorectal adenoma (Ad) and 100 healthy individuals for the present study. The serum expression of miR-196b in sera samples of the three cohorts was detected using reverse transcription-quantitative polymerase chain reaction. The diagnostic value of miR-196b in the serum of the patients with CRC was evaluated by receiver operating characteristic (ROC) curve and survival analysis, using the Kaplan-Meier method, which was performed with the data from a 5-year follow-up. The expression of miR-196b in the serum of patients with CRC was significantly higher compared with that in Ad patients or healthy individuals (all P<0.001), and the overexpression of serum miR-196b was clearly associated with lymph node invasion, differentiation, and the tumor-lymph nodes-metastasis stage (all P<0.05). ROC curve analysis demonstrated that, comparing patients with CRC with healthy individuals, the area under the curve of serum miR-196b was 0.8135, and its specificity and sensitivity were 63 and 87.38%, respectively, at a diagnostic threshold of −4.785. Patients with CRC of miR-196b-high status had shorter overall survival and disease-free survival rates compared with those of miR-196b-low status. In conclusion, the results of the present study demonstrated that serum miR-196b is upregulated in CRC, and may have an application as a diagnostic and prognostic biomarker for patients with CRC.

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“…It is frequently downregulated and plays a crucial role in oncogenesis, including the association with lower proliferation, invasion, and metastatic potential [24]. In glioma, the expression of miR-16 is low and negatively correlates with malignancy; meanwhile, restoration of miR-16 activity can significantly inhibit glioma cell growth and invasion [15].…”

Section: Discussionmentioning

confidence: 99%

Long Intergenic Noncoding RNA 00152 Promotes Glioma Cell Proliferation and Invasion by Interacting with MiR-16

Chen¹,

Li²,

Gao³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long noncoding RNAs (lncRNAs) are a novel class of protein-noncoding transcripts that are aberrantly expressed in multiple diseases including cancers. LINC00152 has been identified as an oncogene involved in many kinds of cancer; however, its expression pattern and function in human glioma remain unclear. Methods: Quantitative real-time polymerase chain reaction was carried out to measure LINC00152 expression in human glioma cell lines and tissues. CCK-8 and EdU assays were performed to assess cell proliferation, and scratch assays and Transwell assays were used to assess cell migration and invasion, respectively. Luciferase reporter assays were carried out to determine the interaction between miR-16 and LINC00152. In vivo experiments were conducted to assess tumor formation. Results: LINC00152 was found to be significantly upregulated in human glioma cell lines and clinical samples. Knockdown of LINC00152 suppressed glioma cell proliferation, migration, and invasion in vitro. In vivo assays in nude mice confirmed that LINC00152 knockdown inhibits tumor growth. Furthermore, mechanistic investigation showed that LINC00152 binds to miR-16 in a sequence-specific manner and suppresses its expression. miR-16 inhibition strongly attenuated LINC00152 knockdown–mediated suppressive effects on proliferation, migration, and invasion. Moreover, LINC00152 induced BMI1 expression by sponging miR-16; this effect further promoted glioma cell proliferation and invasion. Conclusion: We regard LINC00152 as an oncogenic lncRNA promoting glioma cell proliferation and invasion and as a potential target for human glioma treatment.

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miRNA-15a/16: As Tumor Suppressors and More

Cited by 75 publications

References 65 publications

Serum microRNA expression profiling in patients with multiple system atrophy

Serum microRNA expression profiling in patients with multiple system atrophy

The diagnostic effect of serum miR-196b as biomarker in colorectal cancer

Long Intergenic Noncoding RNA 00152 Promotes Glioma Cell Proliferation and Invasion by Interacting with MiR-16

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