MiRNA-154-5p inhibits cell proliferation and metastasis by targeting PIWIL1 in glioblastoma

Wang, Xiuyu; Sun, Shupeng; Tong, Xiaoguang; Ma, Quanfeng; Di, Hui; Fu, Tao; Sun, Zhen; Cai, Ying; Fan, Wei; Wu, Qiaoli; Li, Yidi; Wang, Qiong; Wang, Jinhuan

doi:10.1016/j.brainres.2017.08.014

Cited by 41 publications

(40 citation statements)

References 25 publications

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“…MiRNAs act as a cancer-promoting or anti-cancer factor through epigenetic regulation and regulation of the expression of processing genes and proteins [20,21]. For example, Wang et al proved miR-154 is down-regulated in glioblastoma while miR-154 overexpression suppresses the growth of glioblastoma cells, which could act as a beneficial therapeutic target [22]. However, Lin et al revealed that miR-154 is overexpressed in RCC specimens and cells, which exaggerates the growth of RCC cells [13].…”

Section: Discussionmentioning

confidence: 99%

Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154

Zhang

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/ b-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/b-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154.

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Section: Discussionmentioning

confidence: 99%

Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154

Zhang

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Discussionmentioning

confidence: 99%

“…MiR-154-5p plays opposite roles in different types of cancers (Wang et al 2017;Lin et al 2018). MiR-154-5p is downregulated in glioma and targets PIWIL1 to suppress tumor growth and metastasis (Wang et al 2017). In contrast, miR-154-5p is overexpressed in renal cell carcinoma and promotes cancer cell migration and proliferation (Lin et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…DLGAP1-AS2 is a recent study has been characterized as an oncogenic lncRNA in glioma (Miao et al 2020), while its role in other cancers is unknown. Our deep sequencing analysis revealed the alteration of DLGAP1-AS2 in HCC and its inverse correlation with miR-154-5p (data not shown), which is also a critical player in cancer biology (Wang et al 2017). This study was therefore carried out to analyze the interactions between DLGAP1-AS2 and miR-154-5p in HCC.…”

Section: Introductionmentioning

confidence: 95%

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LncRNA DLGAP1-AS2 SiRNA Silencing Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Up-regulating MiR-154-5p Through Methylation

Chen

Zhang

et al. 2020

Preprint

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Background:DLGAP1-AS2 has been characterized as an oncogenic lncRNA in glioma. This study was performed to explore the role of DLGAP1-AS2 in hepatocellular carcinoma (HCC). Methods:Expression of DLGAP1-AS2 and miR-154-5p in paired HCC and non-tumor tissues from 62 HCC patients was determined by RT-qPCR. The 62 HCC patients were followed up for 5 years to analyze the prognostic value of DLGAP1-AS2 for HCC. DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression was achieved in HCC cells to study the relationship between them. Methylation of miR-154-5p was analyzed by methylation-specific PCR. Cell proliferation was analyzed by CCK-8 assay.Results: DLGAP1-AS2 was upregulated in HCC and predicted poor survival. MiR-154-5p was downregulated in HCC and inversely correlated with DLGAP1-AS2. In HCC cells, DLGAP1-AS2 siRNA silencing resulted in the upregulation of miR-154-5p and decreased methylation of miR-154-5p gene. Transwell assay showed that, DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression inhibited cell invasion and migration, and the combination of LGAP1-AS2 siRNA silencing and miR-154-5p overexpression showed stronger effects.Conclusion: DLGAP1-AS2 siRNA silencing may inhibit HCC cell migration and invasion by up-regulating miR-154-5p through methylation.

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“…Additionally, they have shown that ectopic overexpression of miR-154 can inhibit progression of glioblastoma. 51 In another study conducted on glioma tissue samples and cell lines, it was determined that PIWIL1 and circular RNA circ_101064 were upregulated and there was an inverse correlation between them and miR-154 expression. Upregulated levels of PIWIL1 and circular RNA circ_101064 were associated with aggressive phenotype of the disease and forced overexpression of miR-154 could repress the progression of glioma through binding to its downstream molecule PIWIL1.…”

Section: Mir-154 and Nervous System Cancersmentioning

confidence: 99%

<p>MicroRNA-154: A Novel Candidate for Diagnosis and Therapy of Human Cancers</p>

Nazarizadeh¹,

Mohammadi²,

Alian³

et al. 2020

OTT

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MicroRNAs (miRNAs) are endogenous, non-coding, single-stranded, tiny RNAs with 21-23 nucleotides that regulate several biological functions through binding to target mRNAs and modulating gene expression at post-transcriptional levels. Recent studies have described crucial roles for miRNAs in pathophysiology of numerous human cancers. They can act as an oncogene and promote cancer or as a tumor suppressor and alleviate the disease. Recently discovered microRNA-154 (miR-154) has been proposed to be involved in multiple physiological and pathological processes including cancer. With this aspect, aberrant expression of miR-154 has been demonstrated in variety of human malignancies, suggesting an important role for miR-154 in tumorigenesis. To be specific, it is considered as a tumor suppressor miRNA and exerts its beneficial effects by targeting several genes. This review systematically summarizes the recent advances done on the role of miR-154 in different cancers and discusses its potential prognostic, diagnostic and therapeutic values.

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MiRNA-154-5p inhibits cell proliferation and metastasis by targeting PIWIL1 in glioblastoma

Cited by 41 publications

References 25 publications

Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154

Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154

LncRNA DLGAP1-AS2 SiRNA Silencing Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Up-regulating MiR-154-5p Through Methylation

<p>MicroRNA-154: A Novel Candidate for Diagnosis and Therapy of Human Cancers</p>

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