MiRNA-141 and miRNA-200b are closely related to invasive ability and considered as decision-making biomarkers for the extent of PLND during cystectomy

Abstract

BackgroundMicroRNAs (miRNAs) are small non-coding RNAs that silence their cognate target genes by specifically binding and cleaving messenger RNAs or inhibiting their translation. In this study, we explored whether miRNA-141 and miRNA-200b are involved in regulation of the invasive ability and epithelial–mesenchymal transition (EMT) of bladder cancer cells in vitro. We also evaluated their potential as biomarkers for deciding the extent of pelvic lymph node dissection (PLND) required during radical cystectomy.MethodsPri- and anti-miR cell lines were constructed. The invasive capacity of the cells was tested using a cell invasion assay. The MMP-2, MMP-9 and EMT-related markers were validated through Western blotting analysis. Seventy-eight urine samples from patients undergoing cystectomy and super-extended lymph node dissection were evaluated by qRT-PCR.ResultsLoss of expression of miRNA-141 and miRNA-200b was associated with increased invasion and migration ability, upregulated MMP-2, MMP-9, vimentin and N-cadherin expression, and downregulated E-cadherin expression in bladder cancer cell lines. Urine miRNA-141 and miRNA-200b levels could discriminate patients with lymph node metastasis from those who were lymph node negative (AUC: 0.704 and 0.674, respectively).ConclusionMiRNA-141 and miRNA-200b play important roles in the invasive ability and EMT phenotype of bladder cancer. Detection of miRNA-141 and miRNA-200b can help to identify patients undergoing cystectomy who are likely to have lymph node metastasis, and therefore those who may benefit from super-extended PLND.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1110-7) contains supplementary material, which is available to authorized users.

miRNA-200c and miRNA-141 as potential prognostic biomarkers and regulators of epithelial-mesenchymal transition in eyelid sebaceous gland carcinoma

“…This observation is in line with recent studies suggesting a lower expression of miRNA-200c in renal cell carcinoma and colorectal cancer 12. Similar studies suggesting low expression of miRNA-141 are available for bladder cancer23 and renal cell carcinoma 24…”

Long noncoding RNA LINC00483/microRNA‐144 regulates radiosensitivity and epithelial–mesenchymal transition in lung adenocarcinoma by interacting with HOXA10

“…In NSCLC, downregulated BCAR4 has been verified to exert an inhibitory effect on migration and metastasis by elevating the expression of E‐cadherin and downregulating that of N‐cadherin and vimentin (N. Li, Gao, & Liu, ). Additionally, it has been validated that upregulation of miR‐141 and miR‐200b expression can lead to MMP‐2, MMP‐9, vimentin, and N‐cadherin reduction, and E‐cadherin elevation in bladder cancer cells (Liu et al, ). Additionally, lncRNA UCA1 interference has been shown to regulate EMT, with increased E‐cadherin but decreased MMP‐7, N‐cadherin, and vimentin expression (Xiao, Wu, & Hu, ), which are consistent with the findings in this experiment.…”

MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα

“…Among the miRNAs, the miR-200 family is proven to be directly associated with tumor metastasis and invasiveness (30)(31)(32). miR-200b, which is one of the members of the miR-200 family, has also been reported to have abnormal expression in various types of cancer, including bladder cancer and gastric carcinoma (33,34) PKCα belongs to the protein kinase C family and has been indicated to be involved in tumorigenesis, invasion, metastasis and proliferation (35)(36)(37). Previous studies of breast cancer indicated that PKCα could serve as an effective therapeutic target due to its notable mediator role in cancer (38)(39)(40).…”