MiRNA-133b promotes the proliferation of human Sertoli cells through targeting GLI3

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor historically known for its toxic responses to man-made pollutants such as dioxin. More recently, the AhR has emerged as a suppressor of inflammation, oxidative stress and apoptosis from cigarette smoke by mechanisms that may involve the regulation of microRNA. However, little is known about the AhR regulation of miRNA expression in the lung in response to inhaled toxicants. Therefore, we exposed Ahr−/− and Ahr+/− mice to cigarette smoke for 4 weeks and evaluated lung miRNA expression by PCR array. There was a dramatic regulation of lung miRNA by the AhR in the absence of exogenous ligand. In response to cigarette smoke, there were more up-regulated miRNA in Ahr−/− mice compared to Ahr+/− mice, including the cancer-associated miRNA miR-96. There was no significant change in the expression of the AhR regulated proteins HuR and cyclooxygenase-2 (COX-2). There were significant increases in the anti-oxidant gene sulfiredoxin 1 (Srxn1) and FOXO3a- predicted targets of miR-96. Collectively, these data support a prominent role for the AhR in regulating lung miRNA expression. Further studies to elucidate a role for these miRNA may further uncover novel biological function for the AhR in respiratory health and disease.

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“…g . miR-137, miR-196a and miR-133b)123435. These data suggest that the AhR is a homeostatic regulator of basal miRNA expression.…”

Section: Discussionmentioning

confidence: 74%

Aryl hydrocarbon receptor (AhR)-dependent regulation of pulmonary miRNA by chronic cigarette smoke exposure

Rogers

Souza

Zago

et al. 2017

Sci Rep

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“…Considering the crucial role of immature SC in spermatogenesis, miRNAs act as important regulators in SC proliferation, maturation, and barrier regulation via the modulation of transcription factors involved in the cell cycle (Jiao et al, ; Ma et al, ; Yao et al, ). miR‐133b promoted SC proliferation by directly targeting the transcription factor GLI family zinc finger 3 and increased cyclin B1 and cyclin D11 expressions (Yao et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Specific expression of miRNAs is found in different periods of mouse testes through gene chip and miRscan, and it has been confirmed that follicle stimulating hormone and androgen can regulate male reproductive activity through the expression of miRNAs in SCs (Mishima et al, ; Nicholls et al, ; Wu, Bao, Wang, Hu, & Xu, ; Yan et al, ). Recent studies have demonstrated that miRNAs repress the expression of SC target genes, thereby regulating SC proliferation, maturation, apoptosis, blood‐testis barrier, and stages of the seminiferous epithelium cycle (Jiao et al, ; Ma et al, ; Yao et al, ). Specific knockdown of Dicer, a central component of RNA interference machinery, downregulates the expression of several key genes in SCs and results in male infertility because of spermatozoa absence and progressive testicular degeneration (Papaioannou et al, ; Papaioannou et al, ).…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Zhang

Yang

Wang

et al. 2019

Molecular Reproduction Devel

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Adenosine monophosphate‐activated protein kinase (AMPK) plays a key role in cellular energy homeostasis and cell proliferation. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. It is unclear to whether miRNAs are involved in AMPK‐regulated Sertoli cell (SC) proliferation. To further understand the regulation of miRNAs in the immature boar SC proliferation, 5‐aminoimidazole‐4‐carboxamide‐1‐β‐D‐ribofuranoside (AICAR) was added to activate AMPK. By an Illumina small RNA deep sequencing, we obtained sequences and relative expression levels of 272 known mature miRNAs, among which 9 miRNAs were significantly upregulated whereas 16 miRNAs were downregulated following the AICAR treatment. The results identified 38 conserved miRNAs, with 8 significantly downregulated miRNAs whereas no upregulated miRNAs. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that miR‐1285 was involved in many activities and pathways associated with cell proliferation via targeting on AMPKα2. We validated that AICAR significantly downregulated miR‐1285 level in SCs. Transfection of miR‐1285 mimic increased the SC viability and cell cycle progression but reduced AMPKα2 mRNA and protein levels, indicating that miR‐1285 is involved in the immature boar SC proliferation via downregulating AMPKα2 expression.

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“…For example, using spermatogonial stem cell line C18-4, we have successfully demonstrated that Nodal plays an essential role in promoting the self-renewal of mouse SSCs via Smad2/3 pathway and Pou5f1 activation (He et al 2009) and GDNF stimulates the proliferation and survival of mouse SSCs by activating Ras/ERK1/2 signaling pathway and transcription factor Fos (He et al 2008). In addition, we have shown that miRNA-133b is uninvolved in the regulation of the human SSCs using a human SSC line (Hou et al 2015) and it specifically enhances the proliferation of human Sertoli cells (Yao et al 2016). Sertoli cell lines, e.g.…”

Section: Spermatocyte Line Immortalized With a Specific Promoter-basementioning

confidence: 98%

“…The establishment of male germ cell and Sertoli cell lines brings new promise for reproductive and regenerative medicine due to the great plasticity of spermatogonia and Sertoli cells. Human SSCs including spermatogonia can differentiate into male gametes (Yang et al 2014a), dedifferentiate to pluripotent ES-like cells (Golestaneh, et al 2009), and transdifferentiate to functional and mature hepatocytes (Chen et al 2016). Therefore, they may bring inspirations for new therapeutic strategies to cure male infertility patients with spermatogonia and other human diseases (e.g.…”

Section: Spermatocyte Line Immortalized With a Specific Promoter-basementioning

confidence: 99%

Establishment and applications of male germ cell and Sertoli cell lines

et al. 2016

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MiRNA-133b promotes the proliferation of human Sertoli cells through targeting GLI3

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Cited by 68 publications

References 38 publications

Aryl hydrocarbon receptor (AhR)-dependent regulation of pulmonary miRNA by chronic cigarette smoke exposure

Aryl hydrocarbon receptor (AhR)-dependent regulation of pulmonary miRNA by chronic cigarette smoke exposure

Identification of microRNAs for regulating adenosine monophosphate‐activated protein kinase expression in immature boar Sertoli cells in vitro

Establishment and applications of male germ cell and Sertoli cell lines

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