MiRNA-130a, a potential endometriosis-inducing factor

Azmy, Osama; El-Garf, Wael

doi:10.1097/01.mjx.0000422790.36924.3d

Cited by 7 publications

(15 citation statements)

References 24 publications

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“…An earlier research group in the national research centre has proved that the blood of women with endometriosis possesses a factor, endometriosis inducing factor (EIF), that has the ability to differentiate stem cells cultured with that blood into endometrial like cells and glands. Rasheed and coworkers studied previously the effect of serum of endometriotic women on human umbilical cord blood stem cells and on the contrary from our study they showed morphological cell changes and concluded that endometriotic women serum may contain a factor that they called it Endometriosis Inducing Factor [33]. The difference in the source of mesnchymal stem cells between their study and ours may contribute for the discrepancy in the results.…”

Section: Disscussioncontrasting

confidence: 78%

“…Several factors may play role in development of endometriosis as including abnormal endometrium, altered peritoneal environment, reduced immune surveillance [53], increased angiogenic capacity [14] and endometrium inducing factor(s) [33]. Long-term endometriotic lesions may develop from endometrial stem/progenitor cells, those that may have been established by more mature transient amplifying progenitor cells.…”

Section: Introductionmentioning

confidence: 99%

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Differentially expressed genes: OCT -4, SOX 2, STAT 3, CDH 1 and CDH 2, in cultured mesenchymal stem cells challenged with serum of women with endometriosis

Salama

Eldeen

Abdel-Rasheed

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Endometriosis is a common chronic gynecological disorder defined as the presence of ectopic functional endometrial tissues, outside uterine cavity, primarily on the pelvic peritoneum and the ovaries. Several studies revealed a correlation between aberrant stem-cell activity in the endometrium and endometriosis. Yet the molecular and cellular behaviors of mesnchymal stem cells in development of endometriosis are hampered by lack of invitro experiments. Our aim was to explore morphological and molecular changes associated with mesenchymal stem cells (MSCs) exposition to serum derived from women with severe endometriosis. Two cell cultures of MSCs isolated from endometrial tissues of two endometriosis-free women. Each cell culture was treated individually with the serum of women with endometriosis (experimental group/n = 7), and serum of women without endometriosis (control group/ n = 4) for 14 days. Quantitative Real-Time PCR was performed later to reveal expression of OCT-4, CDH1 and CDH2, STAT3 and SOX2 genes. Morphologically, cells showed no significant changes. However from molecular point of view, we found increased expression in OCT-4, CDH1 and CDH2. For STAT3 and SOX2 we did not find a significant difference. This study shows that endometriosis serum induced molecular changes in human endometrial MSCs (EnMSCs) that might be related to altered cell behavior which may be a step in differentiation that may be completed invivo by other factors to complete the process of transition. Further researches are needed for optimization to reach differentiation.

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Section: Disscussioncontrasting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Differentially expressed genes: OCT -4, SOX 2, STAT 3, CDH 1 and CDH 2, in cultured mesenchymal stem cells challenged with serum of women with endometriosis

Salama

Eldeen

Abdel-Rasheed

et al. 2018

Journal of Genetic Engineering and Biotechnology

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“…Recently, it was demonstrated that MSCs could lead to the expression of endometriosis in a mouse model. Azmy and Elgarf [13] found that this EIF is miR-130a. Our earlier work has proved that EIF exits in the serum of women suffering from endometriosis [11].…”

Section: Discussionmentioning

confidence: 95%

Multicenter cohort molecular evidence of the presence of endometriosis-inducing factor (mir-130a) as a potent regulator of gene expression in endometriosis

Azmy¹,

Said

El-Nouri³

et al. 2014

Medical Research Journal

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Objective To study the role of endometriosis-inducing factor on the differentiation of cultured stem cells into endometrial cells.Methods This was a multicenter prospective cohort casecontrol study where 323 women were examined, but only 134 infertile women were recruited and compiled with the inclusion criteria. The study group comprised 64 women in whom laparoscopy showed endometriotic lesions, and the control group included 70 women free of endometriotic implants. The women's sera were cocultured with mesenchymal stem cells, which were followed up weekly by quantitative real-time PCR to examine the expression of SPARC (secreted protein, acidic, cysteine-rich) and MYC (myelocytomatosis) genes using the 2 ÀDDCt method.Results By coculturing the serum of women with endometriosis and the control group with stem cells, none of the cultures of the sera of women within the control group showed any changes in the normal expression of either SPARC or MYC gene levels. Furthermore, the stem cells were normally multiplying in the same cell line during the entire duration of the study. Nevertheless, stem cells that were cocultured with sera from women with endometriosis showed upregulation of the SPARC gene mRNA with mean respiratory quotient of 3.534 ± 1.129, whereas the MYC gene mRNA was downregulated with a mean respiratory quotient of 0.488 ± 0.104. ConclusionThe sera of women with endometriosis were able to induce transformation of mesenchymal stem cells into endometrial-like cells on a molecular basis. This evidence supports the endometriosis-inducing factor theory of endometriosis and may have tremendous effect on the therapeutic implications of this debilitating condition.Endometriosis and mir-130a as a potent regulator of gene expression Azmy et al. 3The distribution of mRNA expression of the MYC gene within the study group. There is a decrease in the expression of the MYC gene compared with the control group with a mean RQ of 0.488 ± 0.104. MYC, myelocytomatosis; RQ, respiratory quotient.

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“…In the present study, we investigated the effect of the stressful pathogenic condition of endometriosis, represented in endometriotic serum, on human endometrial mesenchymal stromal/stem cells (eMSCs) with regard to their miRNA expression. This study design was based on our previous observation which revealed that serum of women with endometriosis possibly harbors endometriosis inducing factor(s) (EIF) that can lead to the transformation of MSCs into endometrial like cells and glands in vitro (Rasheed et al, 2010[ 45 ]; Azmy and Elgarf, 2012[ 4 ]; Azmy et al, 2014[ 3 ]).…”

Section: Discussionmentioning

confidence: 99%

“…Later on, by assessing the differential expression of 84 miRNAs in the sera of severe endometriosis cases, we suggested that miR-130a may be EIF mediates the trans-differentiation of MSCs into endometrial-like cells, in addition to regulating gene expression in several endometriosis related biological processes and cell functions (Azmy and Elgarf, 2012[ 4 ]; Azmy et al, 2014[ 3 ]).…”

Section: Introductionmentioning

confidence: 99%

microRNA expression analysis in endometriotic serum treated mesenchymal stem cells

Abdel-Rasheed

Eldeen

Mahmoud

et al. 2017

EXCLI Journal; 16:Doc852; ISSN 1611-2156

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MiRNA-130a, a potential endometriosis-inducing factor

Cited by 7 publications

References 24 publications

Differentially expressed genes: OCT -4, SOX 2, STAT 3, CDH 1 and CDH 2, in cultured mesenchymal stem cells challenged with serum of women with endometriosis

Differentially expressed genes: OCT -4, SOX 2, STAT 3, CDH 1 and CDH 2, in cultured mesenchymal stem cells challenged with serum of women with endometriosis

Multicenter cohort molecular evidence of the presence of endometriosis-inducing factor (mir-130a) as a potent regulator of gene expression in endometriosis

microRNA expression analysis in endometriotic serum treated mesenchymal stem cells

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