miR535 negatively regulates cold tolerance in rice

Shen, Yang; Yang, Junkai; Cai, Xiaoxi; Li, Hongyu; Zhu, Yanming; Jia, Bowei; Sun, Xiaofen

doi:10.1007/s11032-019-1094-0

Cited by 26 publications

(15 citation statements)

References 46 publications

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“…As shown in Figure 1 , the real-time qPCR assay showed that the expression of OsmiR535 could be induced by dehydration, 200 mM NaCl, 10 μM ABA, and 20% PEG treatments. On the other hand, as the coding sequence of OsSPL2/7/11/14/16/18/19 have the OsmiR535 targeting site, their expression level should be inhibited by an elevated OsmiR535 expression level [ 10 ]. However, the expression of OsSPL2 and OsSPL18 were upregulated to be 10-fold higher under drought, 200 mM NaCl, and 20% PEG treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, identifying drought-resistance genes could be beneficial for understanding the genetic mechanism of drought tolerance. OsmiR535 is a single-copy microRNA gene belonging to the miR156/529/535 superfamily in rice [ 6 , 10 ]. These microRNAs modulate plant development and stress responses mainly through interactions with target genes, leading to either translational inhibition or gene silencing.…”

Section: Discussionmentioning

confidence: 99%

“…The OsmiR535 role in abiotic stresses was firstly reported in a microarray study, which demonstrated that OsmiR535 could be induced by cold-stresses [ 9 ]. Recent studies revealed the OsmiR535 negatively regulates cold tolerance in rice, as the overexpression of OsmiR535 aggravated cold-induced cell death and ROS(Reactive oxygen species) accumulation [ 10 ]. OsmiR535 modulates plant height, panicle architecture, and grain length by suppressing the expression of OsSPL7/12/16 [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

Yue

Cao

Liu

2020

Plants

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OsmiR535 belongs to the miR156/miR529/miR535 superfamily, a highly conserved miRNA family in plants. OsmiR535 is involved in regulating the cold-stress response, modulating plant development, and determining panicle architecture and grain length. However, the role that OsmiR535 plays in plant responses to drought and salinity are elusive. In the current study, molecular and genetic engineering techniques were used to elucidate the possible role of OsmiR535 in response to NaCl, PEG(Poly ethylene glycol), ABA(Abscisic acid), and dehydration stresses. Our results showed that OsmiR535 is induced under stressed conditions as compared to control. With transgenic and CRISPR/Cas9 knockout system techniques, our results verified that either inhibition or knockout of OsmiR535 in rice could enhance the tolerance of plants to NaCl, ABA, dehydration and PEG stresses. In addition, the overexpression of OsmiR535 significantly reduced the survival rate of rice seedlings during PEG and dehydration post-stress recovery. Our results demonstrated that OsmiR535 negatively regulates the stress response in rice. Moreover, our practical application of CRISPR/Cas9 mediated genome editing created a homozygous 5 bp deletion in the coding sequence of OsmiR535, demonstrating that OsmiR535 could be a useful genetic editing target for drought and salinity tolerance and a new marker for molecular breeding of Oryza sativa.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

Yue

Cao

Liu

2020

Plants

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“…The overexpression of the Osa-miR319 gene led to increased cold stress tolerance (4 • C) after chilling acclimation (12 • C) of plants as compared to wild-type plants [43,47,48]. The miR319 overexpression lines showed more tolerance to cold stress than the OsPCF5 and OsTCP21 RNAi lines in rice [49].…”

Section: Mirnas In Abiotic Stress Tolerancementioning

confidence: 96%

“…Likewise, several transcription factors and micro-RNAs have been reported related to temperature stress tolerance. In rice, miR319, miR535, miR396b, and ONAC095 were reported to increase cold stress tolerance [47][48][49]147]. Additionally, PIF4 is a key facilitator for auxin biosynthesis and is also required for hypocotyl elongation and high temperature stress tolerance [131].…”

Section: Recent Progresses In Genome Editing For Crop Improvementmentioning

confidence: 99%

Transgenic Breeding Approaches for Improving Abiotic Stress Tolerance: Recent Progress and Future Perspectives

Anwar

Kim

2020

IJMS

107

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The recent rapid climate changes and increasing global population have led to an increased incidence of abiotic stress and decreased crop productivity. Environmental stresses, such as temperature, drought, nutrient deficiency, salinity, and heavy metal stresses, are major challenges for agriculture, and they lead to a significant reduction in crop growth and productivity. Abiotic stress is a very complex phenomenon, involving a variety of physiological and biochemical changes in plant cells. Plants exposed to abiotic stress exhibit enhanced levels of ROS (reactive oxygen species), which are highly reactive and toxic and affect the biosynthesis of chlorophyll, photosynthetic capacity, and carbohydrate, protein, lipid, and antioxidant enzyme activities. Transgenic breeding offers a suitable alternative to conventional breeding to achieve plant genetic improvements. Over the last two decades, genetic engineering/transgenic breeding techniques demonstrated remarkable developments in manipulations of the genes for the induction of desired characteristics into transgenic plants. Transgenic approaches provide us with access to identify the candidate genes, miRNAs, and transcription factors (TFs) that are involved in specific plant processes, thus enabling an integrated knowledge of the molecular and physiological mechanisms influencing the plant tolerance and productivity. The accuracy and precision of this phenomenon assures great success in the future of plant improvements. Hence, transgenic breeding has proven to be a promising tool for abiotic stress improvement in crops. This review focuses on the potential and successful applications, recent progress, and future perspectives of transgenic breeding for improving abiotic stress tolerance and productivity in plants.

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Regulatory Role of Micro-RNAs in Plants Under Challenging Environmental Conditions with Special Focus on Drought and Salinity

Kumar¹,

Kaur

Rajam

2021

Harsh Environment and Plant Resilience

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miR535 negatively regulates cold tolerance in rice

Cited by 26 publications

References 46 publications

OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

Transgenic Breeding Approaches for Improving Abiotic Stress Tolerance: Recent Progress and Future Perspectives

Regulatory Role of Micro-RNAs in Plants Under Challenging Environmental Conditions with Special Focus on Drought and Salinity

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