miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways

Abstract

Abstract. MicroRNAs (miRNAs/miRs) are involved in the regulation of various types of cancer, either as oncogenes or tumor suppressors. miR302a has been reported that it could suppress tumor cell proliferation by inhibiting Akt in prostate cancer. The present study examined the effect of miR302a on proliferation and invasion in esophageal cancer cell lines. The expression levels of miR302a in esophageal cancer cell lines was determined by reverse transcription-polymerase chain reaction. Subsequently, miR302a mimics were transfected into esophageal cancer cells, and cell viability and invasion were assessed by MTT and Transwell assays. In addition, the effects of miR302a on the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathways were investigated by western blot analysis. The results revealed that miR302a expression was significantly decreased in the esophageal cancer cell lines compared with a healthy esophagus epithelium cell line. Upregulation of miR302a inhibited the proliferation and invasion of esophageal cancer cells, and decreased the phosphorylation of extracellular signal-regulated kinase 1/2 and Akt. Taken together, the results of the present study indicated that miR302a overexpression inhibited the proliferation and invasion of esophageal cancer cells through suppression of the MAPK and PI3K/Akt signaling pathways, indicating the potential value of miR302a as a treatment target for human esophageal cancer.

Integrating MicroRNA Expression Profiling Studies to Systematically Evaluate the Diagnostic Value of MicroRNAs in Pancreatic Cancer and Validate Their Prognostic Significance with the Cancer Genome Atlas Data

“…GO term enrichment of biological processes demonstrated that putative miRNA target was the MAPK signaling pathway. Several miRNAs suppressed the MAPK pathway, prohibiting cancer cell proliferation [74,75].…”

E2F4 functions as a tumour suppressor in acute myeloid leukaemia via inhibition of the MAPK signalling pathway by binding to EZH2

“…We found 276 co-altered genes for both shRNAs targeting E2F4 ( Figure 6A). 18,19 The MAPK signalling pathway is also studied in AML. The MAPK signalling pathway is a classic cancer-associated signalling pathway involved in the proliferation and differentiation of tumour cells.…”

“…The MAPK signalling pathway is a classic cancer-associated signalling pathway involved in the proliferation and differentiation of tumour cells. 18,19 The MAPK signalling pathway is also studied in AML. 20,21 To determine the specific mechanism of E2F4-induced tumour cell proliferation and differentiation, we further examined the protein levels of MAPK, p-MAPK, ERK and p-ERK when E2F4 expression levels were manipulated in AML cells.…”

<p>Association Study of <em>MAP3K1</em> SNPs and Risk Factors with Susceptibility to Esophageal Squamous Cell Carcinoma in a Chinese Population: A Case–Control Study</p>

“…30 Further to prove the point, overexpression of miR302a inhibited the viability and invasion of esophageal cancer cells via the MAPK and PI3K/AKT signaling pathways. 31 Several studies also reported that the inhibition of MAPK pathway could enhance target sensitivity to tumor Ag-specific CTL lysis, therefore improving the prognosis in esophageal and gastric cancer patients. 32 To the best of our knowledge, this is the first ever report to study the association between MAP3K1 SNPs and ESCC with or without exposure to risk factors.…”