miR210 modulates respiratory burst in Apostichopus japonicus coelomocytes via targeting Toll-like receptor

Li, Chenghua; Zhao, Mengru; Zhang, Chi; Zhang, Weiwei; Zhao, Xuelin; Duan, Xuemei; Xu, Wei

doi:10.1016/j.dci.2016.08.008

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…Consistent with our previous study ( 16 ), the spatial expression results in this study showed that the highest relative expression levels of AjPAK1 transcripts and protein were obtained in coelomocytes (the major immune-related organ of sea cucumbers) of A. japonicus , suggesting a possible immune-related function of AjPAK1. This observation was similar to those obtained in studies of AjC3 ( 23 ), MyD88 , TRAF6 ( 24 ), IRAK-1 ( 25 ), p105 ( 25 , 26 ), Tollip ( 27 , 28 ), Toll ( 29 ), NF-κB/Rel ( 30 ), TLR3 ( 17 , 29 ), IRAK-1 ( 25 ), Toll ( 31 ), AjBHMT ( 32 ) and AjRhoA ( 19 ) in A. japonicus . In addition, relative expression alterations were observed both in miR-7 and AjPAK1 (mRNA and protein) after V. splendidus infection in A. japonicus , indicating an involvement of both miR-7 and AjPAK1 in response to V. splendidus infection.…”

Section: Discussionsupporting

confidence: 89%

MiR-7 Regulates Pathogen-Induced Immune Response via PAK1 in the Sea Cucumber Apostichopus japonicus

Zhao

Ren

et al. 2022

Front. Immunol.

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MicroRNA-7 (miR-7) is a highly conserved short non-coding RNA involved in various bioprocesses via the regulation of multiple target genes. To enrich our knowledge of the functions of miR-7 in innate immune regulation in echinoderms, we first investigated the targeting relationship between miR-7 and PAK1 in the sea cucumber Apostichopus japonicus and then explored the functions of miR-7, the PAK1 gene, and the miR-7/PAK1 axis in the pathogen-induced immune response of A. japonicus. Our results showed that miR-7 can bind to the 3ʹUTR of PAK1 and negatively regulate the expression of PAK1 in A. japonicus. Overexpression and inhibition of miR-7 and inhibition of the expression of PAK1 can alter phagocytosis, cellular agglutination, and lysozyme contents in A. japonicus. Both miR-7 and the PAK1 gene are involved in immune defense against Vibrio splendidus infection; the miR-7/AjPAK1 axis showed immune regulatory function at 48 to 72 h post-infection (hpi) after V. splendidus infection in A. japonicus. In summary, the results of this study established that miR-7 regulates the pathogen-induced immune response by targeting PAK1 in A. japonicus.

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Section: Discussionsupporting

confidence: 89%

MiR-7 Regulates Pathogen-Induced Immune Response via PAK1 in the Sea Cucumber Apostichopus japonicus

Zhao

Ren

et al. 2022

Front. Immunol.

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“…These effects were eliminated by the knockdown of miR-210. Studies have found that miR-210 has other targets including PLK1, ISCU, Toll-like receptor, and Kruppel-like factor 7 [29][30][31][32]. Expanding on this data, we identified AIFM3 as a novel target of miR-210.…”

Section: Discussionmentioning

confidence: 59%

Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis

et al. 2020

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Background Myocardial infarction (MI) is a major cause of death worldwide. Although percutaneous coronary intervention and coronary artery bypass grafting can prolong life, cardiac damage persists. In particular, cardiomyocytes have no regenerative capacity. Mesenchymal stem cells (MSCs) are attractive candidates for the treatment of MI. The manner by which MSCs exert a beneficial effect upon injured cells is a source of continued study. Methods After the isolation and identification of exosomes from MSCs, the expression of miR-210 was determined by microarray chip. Subsequently, gain- and loss-function approaches were conducted to detect the role of exosomes and exosomal-miR-210 in cell proliferation and apoptosis of cardiomyocytes, as well as the MI in vivo. Dual-Luciferase Report Gene System was used to demonstrate the target gene of miR-210. Results We tested the hypothesis that MSC-derived exosomes transfer specific miRNA to protect cardiomyocytes from apoptotic cell death. Interestingly, direct cardiac injection of MSC exosomes reduced infarct size and improved heart function after coronary ligation. In vitro, the MSC exosomes enhanced cardiomyocyte survival to hypoxia. Confirmation of exosome uptake in myocytes was confirmed. Dual-luciferase reporter assay implicated miR-210 as a mediator of the therapeutic effect and AIFM3 as a downstream target. Treatment with miR-210 overexpressing MSC exosomes improved myocyte protection to both in vitro and in vivo stress. Furthermore, the endogenous and exogenous miR-210 had the same therapeutic effects. Conclusion These results demonstrated that the beneficial effects offered by MSC-exosomes transplantation after MI are at least partially because of excreted exosome containing mainly miR-210. Graphical abstract

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“…MiRNAs served as crucial post-transcriptional regulators of gene expression by transcriptional degradation of target mRNA or inhibition of protein synthesis (Li et al, 2016; Miao et al, 2017; Shao et al, 2017). In order to reveal the microRNA/mRNA regulatory relationship, we predicted that miR-125a could target Wnt2, belonging to the Wnt genes family.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs Profiling Identifies miR-125a and Its Target Gene Wnt2 in Skins of Different Haired Rabbits

et al. 2018

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MicroRNAs (miRNAs) play critical roles in the control of skin and hair follicle development, epidermal homeostasis and pigmentation. However, the roles of miRNAs in the skins of rabbits with different hair types are unclear. In this study, we profiled miRNAs in the skins of long and short haired rabbits by Illumina deep sequencing. The dataset was compared with known mammalian miRNAs in miRBase 21.0. In total, 118 miRNAs were found to be differentially expressed between the two different rabbit types, of which 94 were upregulated, and 24 were downregulated in the skin of short haired vs. long haired rabbits. The expression levels of five randomly selected miRNAs detected by quantitative real-time PCR indicated that the expression patterns were consistent with Illumina sequencing results. What’s more, bioinformatics analysis showed that miR-125a might target Wnt2, an important modulator for hair follicle development. To test whether Wnt2 is a target of miR-125a, luciferase reporter vector (pMir-report-Wnt2-3′-UTR-WT) and its substitution mutant (pMir-report-Wnt2-3′-UTR-MUT) were constructed. Co-transfection and reporter enzyme assays showed that compared with control (miR-125a NC transfection), miR-125a mimics transfection significantly inhibited the reporter luciferase activities expressed by pMir-report-Wnt2-3′-UTR-WT, while transfection of miR-125a inhibitors increased reporter enzyme activities. RT-PCR and Simple Western analysis found that Wnt2 mRNA and protein levels were induced or repressed by miR-125a overexpression or inhibition, respectively. Moreover, the mRNA expression levels of genes in Wnt signaling pathway, such as CTNNB1, LEF-1, PPARD and TGFB1, were also significantly changed (P < 0.05), consistent with Wnt2. It indicated that the regulation of Wnt2 expression by miRNAs may depend on the transcriptional degradation. The results will help to further understand the role of miRNAs in hair follicle development and the genetic mechanism underlying hair length phenotype.

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miR210 modulates respiratory burst in Apostichopus japonicus coelomocytes via targeting Toll-like receptor

Cited by 23 publications

References 30 publications

MiR-7 Regulates Pathogen-Induced Immune Response via PAK1 in the Sea Cucumber Apostichopus japonicus

MiR-7 Regulates Pathogen-Induced Immune Response via PAK1 in the Sea Cucumber Apostichopus japonicus

Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis

MicroRNAs Profiling Identifies miR-125a and Its Target Gene Wnt2 in Skins of Different Haired Rabbits

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