miR167 limits anther growth to potentiate anther dehiscence

Zheng, Lanjie; Nagpal, Punita; Villarino, Gonzalo H.; Trinidad, Brendan; Bird, Laurina; Huang, Yubi; Reed, Jason W.

doi:10.1242/dev.174375

Cited by 27 publications

(23 citation statements)

References 91 publications

(155 reference statements)

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“…The accumulation of miRNA167 was significantly reduced in hen1 mutants (Yu et al, 2010;Ren et al, 2012). Consistently, ARF6 and ARF8, major targets of miRNA167 (Nagpal et al, 2005;Wu et al, 2006;Yao et al, 2019;Zheng et al, 2019), were ectopically expressed in hen1-8 (Figure 7). However, introducing arf6 or arf8 did not suppress developmental defects of hen1-8 ovules (Supplementary Figure S9).…”

Section: Discussionsupporting

confidence: 54%

HUA ENHANCER1 Mediates Ovule Development

et al. 2020

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Ovules are female reproductive organs of angiosperms, containing sporophytic integuments and gametophytic embryo sacs. After fertilization, embryo sacs develop into embryos and endosperm whereas integuments into seed coat. Ovule development is regulated by transcription factors (TF) whose expression is often controlled by microRNAs. Mutations of Arabidopsis DICER-LIKE 1 (DCL1), a microRNA processing protein, caused defective ovule development and reduced female fertility. However, it was not clear whether other microRNA processing proteins participate in this process and how defective ovule development influenced female fertility. We report that mutations of HUA ENHANCER1 (HEN1) and HYPONASTIC LEAVES 1 (HYL1) interfered with integument growth. The sporophytic defect caused abnormal embryo sac development and inability of mutant ovules to attract pollen tubes, leading to reduced female fertility. We show that the role of HEN1 in integument growth is cell-autonomous. Although AUXIN RESPONSE FACTOR 6 (ARF6) and ARF8 were ectopically expressed in mutant ovules, consistent with the reduction of microRNA167 in hen1, introducing arf6;arf8 did not suppress ovule defects of hen1, suggesting the involvement of more microRNAs in this process. Results presented indicate that the microRNA processing machinery is critical for ovule development and seed production through multiple microRNAs and their targets.

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Section: Discussionsupporting

confidence: 54%

HUA ENHANCER1 Mediates Ovule Development

et al. 2020

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“…Arabidopsis miR167 is required for normal anther and ovule development through negative regulation of auxin response factors ARF6 and ARF8 (Wu et al, 2006;Zheng et al, 2019). Overexpression of ARF6 or ARF8 in Arabidopsis increased auxin response which impaired anther and ovule development, similar to the phenotypes observed in miR167 mutants (Wu et al, 2006;Yao et al, 2019).…”

Section: Non-coding Rnas Associated With Plant Hormones and Secondary Metabolites Contribute To Ecodormancy Or Bud Breakmentioning

confidence: 68%

Genome-Wide Changes of Regulatory Non-Coding RNAs Reveal Pollen Development Initiated at Ecodormancy in Peach

Bennett

Dardick

et al. 2021

Front. Mol. Biosci.

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Bud dormancy is under the regulation of complex mechanisms including genetic and epigenetic factors. To study the function of regulatory non-coding RNAs in winter dormancy release, we analyzed the small RNA and long non-coding RNA (lncRNA) expression from peach (Prunus persica) floral buds in endodormancy, ecodormancy and bud break stages. Small RNAs underwent a major shift in expression primarily between dormancy and flowering with specific pairs of microRNAs and their mRNA target genes undergoing coordinated differential expression. From endodormancy to ecodormancy, ppe-miR6285 was significantly upregulated while its target gene, an ASPARAGINE-RICH PROTEIN involved in the regulation of abscisic acid signaling, was downregulated. At ecodormancy, ppe-miR2275, a homolog of meiosis-specific miR2275 across angiosperms, was significantly upregulated, supporting microsporogenesis in anthers at a late stage of dormancy. The expression of 785 lncRNAs, unlike the overall expression pattern in the small RNAs, demonstrated distinctive expression signatures across all dormancy and flowering stages. We predicted that a subset of lncRNAs were targets of microRNAs and found 18 lncRNA/microRNA target pairs with both differentially expressed across time points. The genome-wide differential expression and network analysis of non-coding RNAs and mRNAs from the same tissues provide new candidate loci for dormancy regulation and suggest complex noncoding RNA interactions control transcriptional regulation across these key developmental time points.

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“…Arabidopsis arf6; arf8 double mutants show more severe floral phenotypes than either single mutants, suggesting partial redundancy between AtARF6 and AtARF8 (Nagpal et al, 2005). In Arabidopsis and tomato, over-expression of miR167, which targets both ARF6 and ARF8, also caused similar phenotypes as the arf6; arf8 double mutants (Wu et al, 2006; Liu et al, 2014; Zheng et al, 2019). Most relevant to this work, Arabidopsis arf8 mutants develop parthenocarpic fruit, and the expression of an “dominant negative” AtARF8 causes parthenocarpy in Arabidopsis and tomato (Goetz et al, 2007).…”

Section: Discussionmentioning

confidence: 89%

Mechanism of auxin and GA crosstalk in diploid strawberry fruit initiation

Zhou

Sittmann

Guo

et al. 2020

Preprint

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Strawberry, a high value fruit crop, has recently become amenable for genetic studies due to genomic resources and CRISPR/CAS9 tools. Unlike ovary-derived botanical fruits, strawberry is an accessory fruit derived from receptacle, the stem tip subtending floral organs. Although both botanical and accessory fruits initiate development in response to auxin and GA released from seeds, the downstream auxin and GA signaling mechanisms underlying accessory fruit development remain unknown. Using wild strawberry, we performed in depth molecular characterizations of accessory fruit development. We show that auxin signaling proteins FveARF8/FveARF6 are bound and hence inhibited by FveIAA4 and FveRGA1, repressors in auxin and GA signaling pathways. This inhibition is relieved post-fertilization or by the application of GA or auxin. Mutants of FveRGA1 developed parthenocarpic fruit suggesting a conserved function of DELLA proteins in fruit set. Further, FveARF8 was found to repress the expression of a GA receptor gene GID1c to control fruit’s sensitivity to GA, revealing a novel crosstalk mechanism. We demonstrate that consensus co-expression network provides a powerful tool for non-model species in the selection of interacting genes for functional studies. These findings will facilitate the improvement of strawberry fruit productivity and quality by guiding future production of parthenocarpic fruit.One sentence summaryGenome editing and network analysis are applied to investigating the mechanism of accessory fruit initiation in the wild strawberry, revealing conserved as well as novel crosstalk mechanisms.The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Zhongchi Liu (zliu@umd.edu)

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miR167 limits anther growth to potentiate anther dehiscence

Cited by 27 publications

References 91 publications

HUA ENHANCER1 Mediates Ovule Development

HUA ENHANCER1 Mediates Ovule Development

Genome-Wide Changes of Regulatory Non-Coding RNAs Reveal Pollen Development Initiated at Ecodormancy in Peach

Mechanism of auxin and GA crosstalk in diploid strawberry fruit initiation

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