miR156-SPL modules regulate induction of somatic embryogenesis in citrus callus

Jiang, Long; Liu, Chaoyang; Feng, Meng-Qi; Liu, Yun; Wu, Xiaomeng; Guo, Wen‐Wu

doi:10.1093/jxb/ery132

Cited by 88 publications

(62 citation statements)

References 91 publications

(126 reference statements)

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“…Most of the SPL genes in Arabidopsis are targeted by miR156 and miR157 [14,56,57], and positively regulate downstream flowering genes directly, such as SOC1, LFY, AP1, FUL, and so on [35,51]. This regulation pattern has also been verified in other species [28,53,58,59]. In this study, we found some SPL binding sites (GTAC) in the promoter regions of EjLFY, EjSOC1, and EjAP1 (Sequence S2); and the dual-luciferase reporter assays verified that EjSPLs activate their expression, in which, EjSPL3, EjSPL4, EjSPL5, and EjSPL9 effectively activated EjLFY-1 and EjSOC1-1 expression; and EjSPL4, EjSPL5, and EjSPL9 significantly improve the expression of EjAP1-1 (Figure 7).…”

Section: Discussionmentioning

confidence: 63%

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

Jiang

Peng

Wang

et al. 2019

IJMS

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The age pathway is important for regulating flower bud initiation in flowering plants. The major regulators in this pathway are miR156 and SPL transcription factors. To date, SPL genes have been identified in many species of plants. Loquat, as a woody fruit tree of Rosaceae, is unique in flowering time as it blooms in winter. However, the study of its SPL homologous genes on the regulation mechanism of flowering time is still limited. In this study, four SPL homologs—EjSPL3, EjSPL4, EjSPL5, and EjSPL9—are cloned from loquat, and phylogenetic analysis showed that they share a high sequence similarity with the homologues from other plants, including a highly conserved SQUAMOSA promoter binding protein (SBP)-box domain. EjSPL3, EjSPL4, EjSPL5 are localized in the cytoplasm and nucleus, and EjSPL9 is localized only in the nucleus. EjSPL4, EjSPL5, and EjSPL9 can significantly activate the promoters of EjSOC1-1, EjLFY-1, and EjAP1-1; overexpression of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in wild-type Arabidopsis thaliana can promote flowering obviously, and downstream flowering genes expression were upregulated. Our work indicated that the EjSPL3, EjSPL4, EjSPL5, and EjSPL9 transcription factors are speculated to likely participate in flower bud differentiation and other developmental processes in loquat. These findings are helpful to analyze the flowering regulation mechanism of loquat and provide reference for the study of the flowering mechanism of other woody fruit trees.

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Section: Discussionmentioning

confidence: 63%

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

Jiang

Peng

Wang

et al. 2019

IJMS

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“…An example of such regulation was described for micropropagated strawberry, where during in vitro culture, the miR156 is upregulated whereas other miRNAs (miR164, miR172, and miR390) are down-regulated (Li et al 2012). miR156 is one of the eminently conserved plant microR-NAs (Axtell and Bowman 2008) and is involved in many fundamental biological processes that include for example plant phase changes (Massoumi et al 2017;Wu et al 2009), stress response (Matthews et al 2019) or regulating somatic embryogenesis induction (Long et al 2018;Szyrajew et al 2017). Thus, under in vitro conditions, various miRNAs even from the same family, may differ in their expression manner.…”

Section: Epigenetic Aspects Of Plant Regeneration Via In Vitro Tissuementioning

confidence: 99%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture. Key message Variation appeared in regenerated plants as well as variation inherited by generative progeny of regenerants can may many, positive or negative impact, of gained plant materials. This review focused on factors that triggered this phenomenon with underlying oxidative stress.

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“…Callus tissues have an advantage of vigorous growth and can be easily collected without season and space limitation. Callus transformation system has been proven to be a rapid and efficient approach to assess gene function in fruit trees such as apple [46][47][48][49] and citrus 50 . In peach, callus tissues induced from various explants such as leaf, stem and calyx have been reported, but there is no report on establishment of callus transformation system.…”

Section: Discussionmentioning

confidence: 99%

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Lai

Zhao

et al. 2020

Sci Rep

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Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach. open Scientific RepoRtS | (2020) 10:2836 | https://doi.

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miR156-SPL modules regulate induction of somatic embryogenesis in citrus callus

Abstract: Overexpression of csi-miR156a enhances the somatic embryogenesis capability of citrus through downstream repression of CsSPL3 and CsSPL14.

Cited by 88 publications

References 91 publications

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

Plant tissue culture environment as a switch-key of (epi)genetic changes

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

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