MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p

Raj, Kul; Liao, Yuan; Cai, Mengjuan; Qiu, Haori; Wen, Faxin; Peng, Min; Wang, Song; Li, Shasha; Guo, Guijie; Chi, Xiaojuan; Maarouf, Mohamed; Chen, Yongqin David; Huang, Shile; Chen, Ji‐Long

doi:10.1128/mbio.02510-22

Cited by 11 publications

(5 citation statements)

References 53 publications

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“…MIR155HG plays a crucial role in regulating innate immunity, far beyond the processing of miR-155. Knockout mouse models lacking most of the MIR155HG sequences and another lacking the core miRNA-155 sequence showed that MIR155HG and miR-155 have distinct functions in immunity, with MIR155HG acting through IFN production and miR-155 acting through the regulation of the STAT1 signaling pathway [66]. While MIR155HG is a direct target of NF-κB that promotes its transcription [22], in a positive feedback mechanism, the MIR155HG/miR-155 axis also regulates NF-κB activity by interfering with its upstream elements or related signaling molecules and induces inflammation and the release of proinflammatory cytokines [67].…”

Section: Discussionmentioning

confidence: 99%

Association of HOTAIR, MIR155HG, TERC, miR-155, -196a2, and -146a Genes Polymorphisms with Papillary Thyroid Cancer Susceptibility and Prognosis

Karajovic,

Kovacevic,

Uzelac

et al. 2024

Cancers

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Polymorphisms in long non-coding RNA and microRNA genes may play a significant role in the susceptibility and progression of papillary thyroid carcinoma (PTC). The current study investigates the polymorphisms HOTAIR rs920778, MIR155HG rs1893650, TERC rs10936599, miR-155 rs767649, miR-196a2 rs11614913 and miR-146a rs2910164 in 102 PTC patients and 106 age- and sex-matched controls of the Caucasian Serbian population, using real-time PCR. We observed differences in genotype distributions of the HOTAIR rs920778 (p = 0.016) and MIR155HG rs1893650 (p = 0.0002) polymorphisms between PTC patients and controls. HOTAIR rs920778 was associated with increased PTC susceptibility (adjusted OR = 1.497, p = 0.021), with the TT variant genotype increasing the risk compared to the CC genotype (OR = 2.466, p = 0.012) and C allele carriers (CC + CT) (OR = 1.585, p = 0.006). The HOTAIR rs920778 TT genotype was associated with lymph node metastasis (p = 0.022), tumor recurrence (p = 0.016), and progression-free survival (p = 0.010) compared to C allele carriers. Multivariate Cox regression revealed that ATA risk (HR = 14.210, p = 0.000004) and HOTAIR rs920778 (HR = 2.811, p = 0.010) emerged as independent prognostic factors in PTC. A novel polymorphism, MIR155HG rs1893650, was negatively correlated with susceptibility to PTC, with TC heterozygotes exerting a protective effect (OR = 0.268, p = 0.0001). These results suggest that the polymorphisms HOTAIR rs920778 and MIR155HG rs1893650 could be potential prognostic and risk biomarkers in papillary thyroid carcinomas.

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Section: Discussionmentioning

confidence: 99%

Association of HOTAIR, MIR155HG, TERC, miR-155, -196a2, and -146a Genes Polymorphisms with Papillary Thyroid Cancer Susceptibility and Prognosis

Karajovic,

Kovacevic,

Uzelac

et al. 2024

Cancers

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“…Human lung epithelial cells (A549), human embryonic kidney cells (HEK293T/293T), mouse embryo fibroblast cells (NIH/3T3), murine lung epithelial-12 cells (MLE-12), mouse breast cancer cells (4T1), mouse lung adenoma epithelial-4 cells (LA-4), porcine kidney epithelial cells (PK-15), mouse mononuclear macrophage cells (RAW 264.7), chicken embryonic fibroblast cells (DF-1), and MDCK cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Billings, MT) supplemented with 10% (vol/vol) fetal bovine serum (Gibco), 100 U/mL penicillin, and 100 U/mL streptomycin in 37°C incubator under humidified 5% CO 2 as previously described ( 48 ). Recombinant human IFN-β was purchased from PeproTech (PeproTech Inc., Rocky Hill, NJ), and poly(I:C), BAY 11-7082, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Influenza A Virus-Induced circRNA circMerTK Negatively Regulates Innate Antiviral Responses

et al. 2023

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“…There is increasing evidence revealing that long noncoding RNAs (lncRNAs) play critical roles in regulating innate immunity and IAV pathogenesis [37][38][39]. To dissect the autophagy-independent mechanism by which ATG7 promotes the viral replication, we performed RNA-Seq to analyze differentially expressed lncRNAs between control and ATG7 knockdown A549 cells infected with influenza virus A/PR8/34 (H1N1) (Figs 6A and S6A).…”

Section: Identification Of Lncrna Gaplinc As a Critical Regulator Inv...mentioning

confidence: 99%

ATG7/GAPLINC/IRF3 axis plays a critical role in regulating pathogenesis of influenza A virus

Chen,

Guo,

Wang

et al. 2024

PLoS Pathog

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Autophagy-related protein 7 (ATG7) is an essential autophagy effector enzyme. Although it is well known that autophagy plays crucial roles in the infections with various viruses including influenza A virus (IAV), function and underlying mechanism of ATG7 in infection and pathogenesis of IAV remain poorly understood. Here, in vitro studies showed that ATG7 had profound effects on replication of IAV. Depletion of ATG7 markedly attenuated the replication of IAV, whereas overexpression of ATG7 facilitated the viral replication. ATG7 conditional knockout mice were further employed and exhibited significantly resistant to viral infections, as evidenced by a lower degree of tissue injury, slower body weight loss, and better survival, than the wild type animals challenged with either IAV (RNA virus) or pseudorabies virus (DNA virus). Interestingly, we found that ATG7 promoted the replication of IAV in autophagy-dependent and -independent manners, as inhibition of autophagy failed to completely block the upregulation of IAV replication by ATG7. To determine the autophagy-independent mechanism, transcriptome analysis was utilized and demonstrated that ATG7 restrained the production of interferons (IFNs). Loss of ATG7 obviously enhanced the expression of type I and III IFNs in ATG7-depleted cells and mice, whereas overexpression of ATG7 impaired the interferon response to IAV infection. Consistently, our experiments demonstrated that ATG7 significantly suppressed IRF3 activation during the IAV infection. Furthermore, we identified long noncoding RNA (lncRNA) GAPLINC as a critical regulator involved in the promotion of IAV replication by ATG7. Importantly, both inactivation of IRF3 and inhibition of IFN response caused by ATG7 were mediated through control over GAPLINC expression, suggesting that GAPLINC contributes to the suppression of antiviral immunity by ATG7. Together, these results uncover an autophagy-independent mechanism by which ATG7 suppresses host innate immunity and establish a critical role for ATG7/GAPLINC/IRF3 axis in regulating IAV infection and pathogenesis.

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MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p

Cited by 11 publications

References 53 publications

Association of HOTAIR, MIR155HG, TERC, miR-155, -196a2, and -146a Genes Polymorphisms with Papillary Thyroid Cancer Susceptibility and Prognosis

Association of HOTAIR, MIR155HG, TERC, miR-155, -196a2, and -146a Genes Polymorphisms with Papillary Thyroid Cancer Susceptibility and Prognosis

Influenza A Virus-Induced circRNA circMerTK Negatively Regulates Innate Antiviral Responses

ATG7/GAPLINC/IRF3 axis plays a critical role in regulating pathogenesis of influenza A virus

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