MiR-let-7d-3p inhibits granulosa cell proliferation by targeting TLR4 in polycystic ovary syndrome

Wu, Wei; Duan, Cuicui; Lv, Houyi; Song, Junxian; Cai, Wei-Xiong; Fu, Kaiyou; Xu, Jian

doi:10.1016/j.reprotox.2021.10.003

Cited by 7 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Very few miRs were similarly modified across age or sex, particularly for day 1. For some miRs that were consistently downregulated in young males on day 1 and day 4 (miR-let-7d-3p) or downregulated in young males and females on day 4 (miR-383-5p), previous research suggest that downregulation is neuroprotective against inflammation and associated oxidative stress [41,42,61]. Similarly, for miRs that exhibited an increase on day 4, in young and old septic males (miR-223-3p, miR-544-5p, miR-219a-5p, miR-15a-5p), the increased expression has been linked to inhibition of inflammation and neuroprotection [47][48][49][50][51][52][53][54].…”

Section: Figure 3 Direction Of Mrna Expression Associated With Increased Mir Expression On Day 1 and Day 4 Post-sepsismentioning

confidence: 98%

“…On day 4 following sepsis, the number of downregulated miRs increased to 30 and included 2, miR-335-3p and miRlet-7d-3p, which were also decreased on day 1. Expression of miR-let-7d-3p has been linked the regulation of the inflammatory response [40,41] and expression of miR-335-3p has been linked to the response to stress [42] and regulation of neuroprotection and neurodegeneration [43,44]. In addition, elevated expression of miR-335-3p has been associated with poorer memory during aging [45] and inhibition of estrogen receptor expression [46].…”

Section: Young Adult Male Micementioning

confidence: 99%

See 1 more Smart Citation

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Rani

Barter

Kumar

et al. 2022

Aging

View full text Add to dashboard Cite

Sepsis, defined as a dysregulated host immune response to infection, is a common and dangerous clinical syndrome. The excessive host inflammatory response can induce immediate and persistent cognitive decline, which can be worse in older individuals. Sex-specific differences in the outcome of infectious diseases and sepsis appear to favor females. We employed a murine model to examine the influence of age and sex on the brain's microRNA (miR) response following sepsis. Young and old mice of both sexes underwent cecal ligation and puncture (CLP) with daily restraint stress. Expression of hippocampal miR was examined in age-and sex-matched controls at 1 and 4 days post-CLP. Few miR were modified in a similar manner across age or sex and these few miR were generally associated with neuroprotection against inflammation. Similar to previous work examining transcription, young females exhibited a better recovery of the miR profile from day 1 to day 4, relative to young males and old females. For young males and all female groups, the initial response mainly involved a decrease in miR expression. In contrast, old males exhibited only upregulated miR on day 1 and day 4 and many of the miR upregulated on day 1 and day 4 were linked to neurodegeneration, increased neuroinflammation, and cognitive impairment. The results emphasize age and sex differences in epigenetic mechanisms that likely contribute to susceptibility or resilience to cognitive impairment due to sepsis.

show abstract

Section: Figure 3 Direction Of Mrna Expression Associated With Increased Mir Expression On Day 1 and Day 4 Post-sepsismentioning

confidence: 98%

Section: Young Adult Male Micementioning

confidence: 99%

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Rani

Barter

Kumar

et al. 2022

Aging

View full text Add to dashboard Cite

show abstract

“…Additionally, hsa-let-7d-3p is a let-7 family subtype that can control a large number of immune genes. Its targets, such as TLR4 and AGO1, played significant roles in several immune responses (Wu et al, 2021). Hsa-let-7 was highly expressed in patients with fibromyalgia and closely related to intraepidermal nerve fiber density and chronic pain (Leinders et al, 2016).…”

Section: Martinezmentioning

confidence: 99%

Integrated omics analysis reveals the epigenetic mechanism of visceral hypersensitivity in IBS-D

Chai

Qiu

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Background and objective: IBS-D is a common functional bowel disease with complex etiology and without biomarker. The pathological and physiological basis of IBS-D focuses on visceral hypersensitivity. However, its epigenetic mechanism remains elusive. Our study aimed to integrate the relationship between differentially expressed miRNAs, mRNAs and proteins in IBS-D patients in order to reveal epigenetic mechanism of visceral hypersensitivity from transcription and protein levels and provide the molecular basis for discovering biomarkers of IBS-D.Methods: The intestinal biopsies from IBS-D patients and healthy volunteers were obtained for high-throughput sequencing of miRNAs and mRNAs. The differential miRNAs were selected and verified by q-PCR experiment followed by target mRNA prediction. Biological functions were respectively analyzed for target mRNAs, differential mRNAs and the previously identified differential proteins in order to explore the characteristic involved visceral hypersensitivity. At last, interaction analysis of miRNAs, mRNAs and proteins was performed for the epigenetic regulation mechanism from transcription and protein levels.Results: Thirty-three miRNAs were found to be differentially expressed in IBS-D and five of them were further confirmed, including upregulated hsa-miR-641, hsa-miR-1843, hsa-let-7d-3p and downregulated hsa-miR-219a-5p, hsa-miR-19b-1-5p. In addition, 3,812 differential mRNAs were identified. Thirty intersecting molecules were found from the analysis on the target mRNAs of miRNAs and mRNAs. Fourteen intersecting molecules were obtained from the analysis on the target mRNAs and proteins, and thirty-six intersecting molecules were identified from analysis on the proteins and different mRNAs. According to the integrated analysis of miRNA-mRNA-protein, we noticed two new molecules COPS2 regulated by hsa-miR-19b-1-5p and MARCKS regulated by hsa-miR-641. Meanwhile some critical signaling pathways in IBS-D were found such as MAPK, GABAergic synapse, Glutamatergic synapse, and Adherens junction.Conclusion: The expressions of hsa-miR-641, hsa-miR-1843, hsa-let-7d-3p, hsa-miR-219a-5p, and hsa-miR-19b-1-5p in the intestinal tissues of IBS-D patients were significantly different. Moreover, they could regulate a variety of molecules and signaling pathways, which were involved in the multifaceted and multilevel mechanism of visceral hypersensitivity of IBS-D.

show abstract

“…miRNAs have been shown to be widely expressed throughout the body, including organs such as muscles, adipose tissue and ovaries, at the intra-and extracellular levels [1,4]. Recently, miRNAs have been isolated from extracellular fluids with variable expression profile depending on the fluid [1]; urine [10], saliva, plasma/ serum [9] and follicular fluid [11]. Furthermore, miRNAs can be encapsulated in microvesicles [12] which are extracellular hetergeneous membraneous vesicles (EVs) originating from the cells.…”

Section: Mirnas As a Potential Novel Clinical Biomarker For Pcosmentioning

confidence: 99%

The Novelty of miRNAs as a Clinical Biomarker for the Management of PCOS

Alhamdan¹,

Hernandez-Medrano²

2022

Polycystic Ovary Syndrome - Functional Investigation and Clinical Application

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects around 5–10% of women of reproductive age. The aetiology of PCOS is not fully understood with various genetics, iatrogenic (e.g. chemotherapy) and environmental factors have been proposed. microRNAs (miRNAs) are small non-coding single-stranded RNAs which are known to act as a regulator to gene expression at the post-transcriptional levels. Altered expression of miRNAs has been linked to several disorders including infertility. Recent reports demonstrated the expression of differential levels of miRNAs in the serum, ovarian follicular cells and follicular fluid of PCOS patients when compared with healthy women. Therefore, miRNAs may play important role in the pathogenesis of PCOS. The aim of this chapter is to summarise the current understanding pertaining to miRNAs and PCOS and to expedite its possible role in the diagnosis and management of this disorder.

show abstract

MiR-let-7d-3p inhibits granulosa cell proliferation by targeting TLR4 in polycystic ovary syndrome

Cited by 7 publications

References 35 publications

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Integrated omics analysis reveals the epigenetic mechanism of visceral hypersensitivity in IBS-D

The Novelty of miRNAs as a Clinical Biomarker for the Management of PCOS

Contact Info

Product

Resources

About