miR‑96 exerts an oncogenic role in the progression of cervical cancer by targeting CAV‑1

Chen, Yong; Liu, Changqing; Xie, Bingfan; Chen, Shangqiu; Zhuang, Yuan; Zhang, Shaoxia

doi:10.3892/mmr.2020.11101

Cited by 6 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many factors contribute to the posttranscriptional regulation of CAV1, such as microRNA (miRNA), long noncoding RNAs (lncRNA), and circular RNA (circRNA), as well as other proteins. Among them, miRNAs, including miR-199a-5p (Zhong et al, 2020), miR-96 (Chen et al, 2020), miR-124 (Torrejón et al, 2017, miR-103/107 (Zhang et al, 2015), miR-204 (Huang et al, 2019), miR-130a (Wang et al, 2016), and miR-103-3p (Wang et al, 2021), have been demonstrated that to recognize homologous CAV1 mRNA and cause the degradation of CAV1 mRNA or inhibit its translation. In addition, lncRNAs, including lncRNA ANRIL (Zhong et al, 2020), lnc-BMP1-1 (Ling et al, 2020), and lncRNA IMFLNC1 , can regulate the protein expression and function of Cav-1.…”

Section: Regulation Of Caveolin-1 Expressionmentioning

confidence: 99%

Caveolin-1 Regulates Cellular Metabolism: A Potential Therapeutic Target in Kidney Disease

et al. 2021

View full text Add to dashboard Cite

The kidney is an energy-consuming organ, and cellular metabolism plays an indispensable role in kidney-related diseases. Caveolin-1 (Cav-1), a multifunctional membrane protein, is the main component of caveolae on the plasma membrane. Caveolae are represented by tiny invaginations that are abundant on the plasma membrane and that serve as a platform to regulate cellular endocytosis, stress responses, and signal transduction. However, caveolae have received increasing attention as a metabolic platform that mediates the endocytosis of albumin, cholesterol, and glucose, participates in cellular metabolic reprogramming and is involved in the progression of kidney disease. It is worth noting that caveolae mainly depend on Cav-1 to perform the abovementioned cellular functions. Furthermore, the mechanism by which Cav-1 regulates cellular metabolism and participates in the pathophysiology of kidney diseases has not been completely elucidated. In this review, we introduce the structure and function of Cav-1 and its functions in regulating cellular metabolism, autophagy, and oxidative stress, focusing on the relationship between Cav-1 in cellular metabolism and kidney disease; in addition, Cav-1 that serves as a potential therapeutic target for treatment of kidney disease is also described.

show abstract

Section: Regulation Of Caveolin-1 Expressionmentioning

confidence: 99%

Caveolin-1 Regulates Cellular Metabolism: A Potential Therapeutic Target in Kidney Disease

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Further, qPCR analysis of CAV1gene expression in CESC revealed a significant increase in stage II and an insignificant decrease in stage III than the noncancerous cervical tissue (p-value < 0.05; Figure 3H). A recent study on cervical cancer cell lines has reported the decreased level of CAV1 gene expression, and the miR-96 mediated restoration of the CAV1 has resulted in the decrease of cell proliferation, migration, and invasion 43 . Also, other studies have indicated the tumour suppressor role of CAV1 in various cancers, including cervical cancer [44][45][46] .…”

Section: Epithelial Genes Show a Positive Correlation With Mir34b In Cervical Cancermentioning

confidence: 99%

miR-34bassociates with epithelial-mesenchymal transition and regulatesBMP7, CAV1, ID2andFN1in human cervical cancer

Venkatesan

Xavier

Sindhu

et al. 2021

Preprint

View full text Add to dashboard Cite

The emergence of large-scale transcriptomic data provides the opportunity for identifying novel putative targets of microRNAs (miRNAs). In this study, we followed a computational pipeline to predict the candidate gene targets of the miR-34 family. This approach integrates the expressions of miR-34 with genes of heterogeneous primary cervical epithelial squamous cell carcinomas (CESC). Integration of miR-34b and epithelial-mesenchymal transition (EMT) regulated genes has also been focussed, EMT being a reversible process that fuels cancer metastasis. An in-silico approach involving three processes was carried out with CESC datasets of the cancer atlas genome (TCGA), which includes correlation analysis, target prediction database lookup, functional enrichment, network analysis, survival analysis, and EMT score derivation. The results indicate that the miR-34 family may regulate the candidate genes of the mTOR pathway, cell cycle (CCND2) and cell adhesion functions (FZD4). Further, the study reveals the possible regulation of EMT signature genes, namely BMP7, CAV1 and ID2by miR-34b. Further, these transcriptomic signatures were validated in a subset of CESC from the South Asian Indian population (n = 10) and in non-cancerous cervical tissues (n = 5). Upon stably expressing miR-34b in cervical cancer cells (C33A and HeLa), we found repression of these candidate genes and a low negative correlation (r2 = 0.07) between miR-34b and EMT score indicating FN1 as its putative target. Together, these studies revealed the potential targets of the miR-34 family, especially miR-34b, with the hope that they would emerge as potential biomarkers and/or promising therapeutic targets in CESC.

show abstract

“…MicroRNAs (miRNAs) are small, non-coding RNA molecules that are associated with the progression of many cancers including CC, serving as tumor promoters or inhibitors [ 11 , 12 ]. For instance, miR-96 served as a tumor promoter in proliferative, migratory and invasive abilities of CC cells by targeting CAV-1 [ 13 ]. While Kan et al found that the miR-1294 level was declined and impeded cell viability and metastasis by interacting with FLOT1 [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1

Wang

Du²,

Xie

et al. 2022

Reprod. Sci.

View full text Add to dashboard Cite

Cervical cancer (CC) is a leading cause of high morbidity and mortality in women worldwide. Circular RNAs (circRNAs) are considered to be essential regulators of various cancers, including CC. The purpose of this study was to investigate the role and mechanism of circ_0005576 in CC progression. The levels of circ_0005576, miR-1305, and poly(A)-binding protein-interacting protein 1 (PAIP1) were detected by quantitative real-time PCR (qRT-PCR) or western blot assay. The stability and location of circ_0005576 were determined by ribonuclease R (RNase R) assay and subcellular fractionation distribution assay, respectively. Cell proliferation was evaluated by CCK-8 assay, EDU incorporation assay, and colony formation assay. Cell migration and invasion were assessed by transwell assay. The interactions between miR-1305 and circ_0005576 or PAIP1 were validated by dual-luciferase reporter assay. The protein expression of cyclin D1, vimentin, and matrix metallopeptidase 9 (MMP9) was tested by western blot. Moreover, mice xenograft models were constructed to analyze tumor growth in vivo. Circ_0005576 and PAIP1 were upregulated, while miR-1305 was downregulated in CC tissues and cells. Circ_0005576 was a stable circRNA that was mainly distributed in the cytoplasm of cells. Knockdown of circ_0005576 suppressed the proliferation, migration, and invasion of CC cells, while the silence of miR-1305 facilitated the development of CC cells. Meanwhile, circ_0005576 could sponge miR-1305 to promote PAIP1 expression. Furthermore, PAIP1 overexpression relieved the influence of circ_0005576 silence on the growth of CC cells. Additionally, circ_0005576 silence hindered CC tumor growth in vivo. Circ_0005576 depletion suppressed tumor development in CC by regulating the miR-1305/PAIP1 axis, suggesting that circ_0005576 might be a potential biomarker for CC treatment.

show abstract

miR‑96 exerts an oncogenic role in the progression of cervical cancer by targeting CAV‑1

Cited by 6 publications

References 34 publications

Caveolin-1 Regulates Cellular Metabolism: A Potential Therapeutic Target in Kidney Disease

Caveolin-1 Regulates Cellular Metabolism: A Potential Therapeutic Target in Kidney Disease

miR-34bassociates with epithelial-mesenchymal transition and regulatesBMP7, CAV1, ID2andFN1in human cervical cancer

Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1

Contact Info

Product

Resources

About