MiR-93/HMGB3 regulatory axis exerts tumor suppressive effects in colorectal carcinoma cells

Gu, Min; Jiang, Zuiming; Li, Huiyuan; Peng, Jun; Chen, Xiang; Tang, Manling

doi:10.1016/j.yexmp.2021.104635

Cited by 10 publications

(5 citation statements)

References 30 publications

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“… 38 miR-93 targeted regulated HMGB3 expression, and the repression effect of miR-93 on biological behavior of CRC cells could be counteracted by HMGB3 overexpression. 39 In the current work, we proved that miR-664b-3p could directly target and negatively regulate HMGB3 expression ( Figure 5 ). Meanwhile, miR-664b-3p enrichment weakened cell proliferation, cell migration and tube formation abilities, accelerated cell apoptosis in CRC cells, and the above effects were reversed due to HMGB3 over-expression ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 53%

CAFs-derived small extracellular vesicles circN4BP2L2 promotes proliferation and metastasis of colorectal cancer via miR-664b-3p/HMGB3 pathway

Yang

Zhang

Luo

et al. 2022

Cancer Biology & Therapy

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Our previous research has demonstrated that colorectal cancer (CRC) progression was promoted by circN4BP2L2. This study aimed to further explore the mechanism of circN4BP2L2 in the development of CRC from the perspective of small extracellular vesicles (sEVs). Cancer-associated fibroblasts cell (CAFs) and normal fibroblasts cell (NFs) were isolated from CRC tissues and adjacent tissues, respectively. The ultra-centrifugation was used for extraction of their related sEVs. Cell proliferation and apoptosis were analyzed using CCK-8 and flow cytometry, respectively. Transwell assay was conducted to measure cell migration. The tube formation ability was assessed by tube formation assay. The target relationships between circN4BP2L2 and miR-664b-3p, and miR-664b-3p and HMGB3 were validated by dual-luciferase reporter detection. The effect of CAFs-derived sEV (CAFs-sEVs) circN4BP2L2 on CRC was further studied in nude mice. CAFs-exo promoted cell proliferation, migration, tube formation ability, and inhibited apoptosis of CRC cells. CAFs-sEV circN4BP2L2 knockdown reversed the above results. CircN4BP2L2 directly targeted miR-664b-3p, and HMGB3 was targeted by miR-664b-3p. Moreover, subcutaneous tumorigenesis and liver metastasis of nude mice with CRC were repressed by CAFs-sEV circN4BP2L2 knockdown. CAFs-sEV circN4BP2L2 knockdown restrained CRC cell proliferation and migration by regulating miR-664b-3p/HMGB3 pathway.

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Section: Discussionmentioning

confidence: 53%

CAFs-derived small extracellular vesicles circN4BP2L2 promotes proliferation and metastasis of colorectal cancer via miR-664b-3p/HMGB3 pathway

Yang

Zhang

Luo

et al. 2022

Cancer Biology & Therapy

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“…To date, the involvement of hsa-miR-93 in CRC cell proliferation, migration, angiogenesis, angiogenesis, and resistance to radiotherapy has been reported [72][73][74] . Moreover, several studies described that hsa-miR-93 could regulate Wnt/β-catenin 75,76 and PI3K/AKT signaling pathways 77 .…”

Section: Mirnasmentioning

confidence: 99%

Integrated whole transcriptome profiling of circRNAs reveals a convoluted crosstalk in competing endogenous RNAs regulatory network in Colorectal Cancer

Mollanoori

Ghelmani

Hassani

et al. 2023

Preprint

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Recently it has been identified that circRNAs can act as miRNAs sponge to regulate gene expression in various types of cancers to associate with cancer initiation and progression. The present study aims to identify colorectal cancer-related circRNAs and the underpinning mechanisms of circRNA/miRNA/mRNA networks in the development and progress of Colorectal Cancer. Differentially expressed circRNAs, miRNAs, and mRNAs were identified in GEO microarray datasets using the Limma package of R. Differentially expressed circRNAs analysis resulted in 23 upregulated and 31 downregulated circRNAs. CeRNAs networks were constructed by intersecting the results of predicted and experimentally validated databases, circbank and miRWalk, and DEMs and DEGs analysis using Cytoscape. Then, the functional enrichment analysis was performed for DEGs included in ceRNA networks. Followed by survival analysis, expression profile validation using TCGA and GEO data, and ROC curve analysis we reached a ceRNA sub-networks which revealed the potential regulatory effect of hsa_circ_0001955 and hsa_circ_0071681 on the survival-related genes, KLF4, MYC, CCNA2, RACGAP1, and CD44. Overall, we constructed a convoluted regulatory network and the likely mechanisms of its action in CRC, which may contribute to developing more effective approaches for early diagnosis, prognosis, and treatment of CRC.

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“…Similarly, this microRNA has been found to be upregulated in gastric cancer ( 162 , 163 ) and expression correlates with poor survival and metastatic disease ( 164 , 165 ), Transfection and overexpression of miRNA-93 in gastric cancer cell lines also promotes an increase in proliferation, migration, invasion and chemoresistance ( 166 , 167 ) and decreases the expression of the tumour suppressors AHNAK ( 167 , 168 ) and TIMP-2 ( 165 ). However, there is conflicting data regarding the expression and contribution of miRNA-93 in colorectal cancer, as expression has been found to be increased in CRC tissue ( 169 ), downregulated by chemotherapeutic drugs ( 170 ) and negatively regulates the expression of the tumour suppressor PTEN in SW620 cells ( 171 ), yet has also been found to be downregulated in CRC tissue ( 172 – 174 ), with suppression associated with metastatic disease and poor overall survival ( 175 ). In addition, other investigations have shown that overexpression of miRNA-93 in CRC cell lines decreases cell viability, increases apoptosis ( 176 ) and can suppress proliferation and colony formation ( 177 ).…”

Section: The Relationship Between Specific Micrornas and Pdcd4 Expres...mentioning

confidence: 99%

The Role and Interactions of Programmed Cell Death 4 and its Regulation by microRNA in Transformed Cells of the Gastrointestinal Tract

Ferris

2022

Front. Oncol.

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Data from GLOBOCAN 2020 estimates that there were 19.3 million new cases of cancer and 10.0 million cancer-related deaths in 2020 and that this is predicted to increase by 47% in 2040. The combined burden of cancers of the gastrointestinal (GI) tract, including oesophageal-, gastric- and colorectal cancers, resulted in 22.6% of the cancer-related deaths in 2020 and 18.7% of new diagnosed cases. Understanding the aetiology of GI tract cancers should have a major impact on future therapies and lessen this substantial burden of disease. Many cancers of the GI tract have suppression of the tumour suppressor Programmed Cell Death 4 (PDCD4) and this has been linked to the expression of microRNAs which bind to the untranslated region of PDCD4 mRNA and either inhibit translation or target the mRNA for degradation. This review highlights the properties of PDCD4 and documents the evidence for the regulation of PDCD4 expression by microRNAs in cancers of the GI tract.

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MiR-93/HMGB3 regulatory axis exerts tumor suppressive effects in colorectal carcinoma cells

Cited by 10 publications

References 30 publications

CAFs-derived small extracellular vesicles circN4BP2L2 promotes proliferation and metastasis of colorectal cancer via miR-664b-3p/HMGB3 pathway

CAFs-derived small extracellular vesicles circN4BP2L2 promotes proliferation and metastasis of colorectal cancer via miR-664b-3p/HMGB3 pathway

Integrated whole transcriptome profiling of circRNAs reveals a convoluted crosstalk in competing endogenous RNAs regulatory network in Colorectal Cancer

The Role and Interactions of Programmed Cell Death 4 and its Regulation by microRNA in Transformed Cells of the Gastrointestinal Tract

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