miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways

Song, Haoming; Zhang, Yao; Liu, Na; Zhao, Sheng; Kong, Yan; Liu, Yuan

doi:10.3390/ijms17111799

Cited by 39 publications

(40 citation statements)

References 51 publications

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“…In a recent study, Song, et al reported that miR-92a-3p can target Notch1 to inhibit glioma. 12 In this study, we also observed the downregulation of Notch1 in EC cells after miR-92a-3p overexpression. Therefore, miR-92a-3p may also target Notch1 in EC.…”

Section: Discussionsupporting

confidence: 63%

“…10 In cancer biology, Notch signaling is dysregulated and plays varied roles in different types of cancers by regulating cancer cell behaviors. 11 In a recent study, Song, et al reported that miR-92a-3p can target Notch1 to participate in glioma, 12 while the interaction between miR-92a-3p and Notch1 in other malignancies remains elusive. LncRNA DCST1-AS1 is recently characterized by oncogenic lncRNA in liver cancer.…”

Section: Introductionmentioning

confidence: 99%

“…LncRNA DCST1-AS1 is recently characterized by oncogenic lncRNA in liver cancer. [12][13][14] Our bioinformatics analysis showed that DCST1-AS1 may interact with miR-92a-3p. This study was therefore carried out to investigate the interaction between DCST1-AS1 and miR-92a-3p in EC.…”

Section: Introductionmentioning

confidence: 99%

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<p>LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1</p>

Ke¹,

Hu²,

Li³

et al. 2020

CMAR

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Introduction: The functions of DCST1-AS1 have been investigated in liver cancer, while its role in endometrial carcinoma (EC) remains hardly known. This study aimed to analyze the role of DCST1-AS1 in EC. Methods: Paired EC and non-tumor tissue samples were obtained from 62 EC patients. These patients were followed up for 5 years since their admission to record their survival conditions. HEC-1 cells were transfected with DCST1-AS1, Notch1 vectors, miRNA negative control or miR-92a-3p mimic. Luciferase activity was measured. QPCR and Western blot were applied to determine the RNA level and protein expression, respectively. The invasion and migration of HEC-1 cells were analyzed by Transwell assay. Results: We in this study found that DCST1-AS1 was upregulated in EC. Survival analysis revealed that high levels of DCST1-AS1 expression predicted poor survival of EC patients. Bioinformatics analysis revealed that miR-92a-3p may bind DCST1-AS1 and the interaction between them was further confirmed by dual-luciferase activity assay. However, overexpression of miR-92a-3p and DCST1-AS1 failed to affect the expression of each other. Moreover, DCST1-AS1 overexpression led to upregulated Notch1 and increased cancer cell invasion and migration rates. Overexpression of miR-92a-3p played an opposite role and attenuated the effects of DCST1-AS1 overexpression. Discussion: DCST1-AS1 is downregulated in EC and may sponge miR-92a-3p, thereby promoting cancer cell invasion and migration.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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<p>LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1</p>

Ke¹,

Hu²,

Li³

et al. 2020

CMAR

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“…In this research, prediction websites revealed that there were potential binding sites among circ_0002232, miR-92a-3p, and PTEN. MiR-92a-3p expression has been revealed to be up-regulated in several solid cancers including breast cancer and brain glioma 30,31 . According to our experiment, miR-92a-3p expression of AML patients was obviously up-regulated compared with controls and was negatively correlated with the expression of circ_0002232.…”

Section: Discussionmentioning

confidence: 99%

Circ_0002232 Acts as a Potential Biomarker for AML and Reveals a Potential ceRNA Network of Circ_0002232/miR-92a-3p/PTEN

Zhu²,

Zhao

et al. 2020

Preprint

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Abstract Background: PTEN, known as a classical tumor suppressor, has been reported to be down-expressed in acute myeloid leukemia (AML) and affected the progression of AML patients. CircRNAs, an emerging type of non-coding RNAs, could act as competing endogenous RNAs (ceRNAs) and has been reported to regulate the expression of PTEN through sponging miRNA in many solid tumors. But there are rarely studies focused on the role of circ-PTEN in AML. Our research was aimed to investigate the expression level of circ_0002232, one of circular RNAs of PTEN, reveal the clinical significance and potential ceRNA interaction network in AML of it.Methods: Circ_0002232 expression in 117 AML patients and 48 controls was detected by using Real-time quantitative PCR. The diagnostic value of circ_0002232 expression was evaluated by receiver operating characteristic curve. Kaplan-Meier curves were used to analyse the impact of circ_0002232 for overall survival. CeRNA network of circ_0002232 was predicted by using interaction prediction websites.Results: Compared with controls, circ_0002232 was notably low-expressed in AML (P<0.001). According to the result of receiver operating characteristic curve, circ_0002232 expression could distinguish AML patients from controls (P<0.001). There were significant differences in patients’ age (P=0.002), FAB classifications (P=0.025), white blood cell count (P=0.034) and platelet count (P=0.047) between low-expressed circ_0002232 group and high-expressed circ_00022332 group. Moreover, there was a positive correlation between circ_0002232 expression and patients’ age (Pearson r=0.256, P=0.0053). Interestingly, we found that patients in low-expressed circ_0002232 group had better overall survival both in whole AML (P=0.019) and non-APL AML (P=0.044). Remarkably, the expression of circ_0002232 was positively correlated with PTEN (Pearson r=0.769, P<0.001). Furthermore, there was a negative correlation in AML between circ_0002232 and miR-92a-3p (Pearson r=-0.262, P=0.032), miR-92a-3p and PTEN (Pearson r=-0.358, P=0.019). Interaction prediction websites revealed that circ_0002232 might regulate the expression of PTEN through sponging miR-92a-3p and affect the process of AML.Conclusions: Circ_0002232, one of circRNAs of PTEN, was remarkably down-regulated in AML and could act as a promising biomarker for the diagnosis of AML. In addition, there might be a potential ceRNA interaction network of circ_0002232/miR-92a-3p/PTEN in AML.

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“…A number of researchers found a similar increase in expression in gliomas and suggested that it can be a marker of glioma (Blümcke et al, 2001). Some researchers argue that MAP2 plays a dual role in the progression of cancer, which was studied in non-glioma tumors (Song et al, 2016). However, some authors assert that MAP2 may even be a positive marker of glioma, as shown in (Yi et al, 2018).…”

Section: Fig 4 Normalized Expression Of Target Genes In Human Gliobmentioning

confidence: 98%

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Panteleev

Pustogarov

Revishchin

et al. 2019

Preprint

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Nucleolus-oriented protein nucleolin plays a significant role in the life of a normal mammalian cell. However, nucleolin is also actively expressed in cells of malignant tumors. At the same time, its expression in different types of cancer is significantly increased compared with normal cells. It is interesting that nucleolin localization often varies in tumor cells, namely in the cytoplasm and on the cell membrane. This fact is considered to be a poor prognostic indicator. This work is devoted to the study of the distribution of nucleolin in human glioblastoma cells. Glioblastoma is one of the most aggressive malignant tumors with an absolutely unfavorable prognosis. These tumors have a high proliferative potential, but in addition they are often characterized by invasive properties. Research on lineage cells does not let to fully study these properties of glioblastoma, since lineage cells are very different from the actual tumor. In our study, we used two primary cell cultures of human glioblastoma with varying degrees of invasiveness of the original tumors. The main interest was directed at studying the localization of nucleolin and its correlation with the invariability of the N- and C-termini of the corresponding protein. Particular attention was paid to the significance of the unaltered C-terminus of nucleolin for its distribution in the cells of transplanted human glioblastoma cultures derived from patient tissues.The aim of this work is to find the relationship between the deformation of the N- or C-terminal sequences of nucleolin and its localization.We showed that in glioblastoma cells, with a high degree of invasion, nucleolin is found in the cytoplasm and close to the cell membrane, and the distribution of nucleolin with undeformed C and N-terminal does not match.

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miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways

Cited by 39 publications

References 51 publications

<p>LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1</p>

<p>LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1</p>

Circ_0002232 Acts as a Potential Biomarker for AML and Reveals a Potential ceRNA Network of Circ_0002232/miR-92a-3p/PTEN

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

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