MiR‐873‐5p modulates progression of tongue squamous cell carcinoma via targeting SEC11A

Abstract

Objective To explore the effect of miR‐873‐5p on proliferation, apoptosis, migration, and invasion of tongue squamous cell carcinoma (TSCC) by targeting SEC11A. Methods Tongue squamous cell carcinoma tissues were collected and performed by qRT‐PCR and Western blotting to determine the expression of miR‐873‐5p and SPC18. SCC9 and CAL‐27 cells were transfected and divided into Mock, mimic NC, miR‐873‐5p mimic, SEC11A, and miR‐873‐5p mimic + SEC11A groups. Then, a series of experiments including cell count kit 8 (CCK‐8), wound healing, Transwell, and flow cytometry were conducted. Besides, Western blotting was used to detect the expression of SPC18 and EGFR pathway‐related proteins. Results MiR‐873‐5p was downregulated while SPC18 was upregulated in TSCC, and miR‐873‐5p was negatively correlated with SPC18. Dual luciferase reporter gene assay confirmed SEC11A to be a target of miR‐873‐5p. Cell proliferation, migration, and invasion of SCC9 and CAL‐27 cells in miR‐873‐5p mimic group were decreased with increased cell apoptosis, presenting with downregulations of SPC18 and EGFR pathway‐related proteins, while cells in SEC11A group manifested totally different changes. Moreover, the inhibitory effect of miR‐873‐5p mimic on TSCC cell growth was abolished by SEC11A overexpression. Conclusion Overexpression of miR‐873‐5p may suppress cell proliferation, migration, and invasion, but facilitate apoptosis in TSCC via targeting SEC11A.

Signal peptidase complex catalytic subunit SEC11A upregulation is a biomarker of poor prognosis in patients with head and neck squamous cell carcinoma

“…SEC11A can promote the phosphorylation of EGFR and the downstream kinases, including ERK and Akt (3). This association was confirmed in tongue squamous cell carcinoma, a major subtype of head and neck squamous cell carcinoma [ 6 ]. EGFR is overexpressed in approximately 50 to 90% of head and neck squamous cell carcinoma cases [ 17 , 18 ].…”

“…Its expression serves as an independent prognostic biomarker for patients with CRC [ 3 ], basal-Like bladder cancer [ 4 ] and esophageal squamous cell carcinoma [ 5 ]. One recent study found that inhibiting SEC11A can reduce proliferation, migration, and invasion and induce apoptosis of tongue squamous cell carcinoma [ 6 ], suggesting a potential oncogenic role of this gene in head and neck squamous cell carcinoma.…”

MiR-873-5p: A Potential Molecular Marker for Cancer Diagnosis and Prognosis

“…Among them, miR-873-5p is up-regulated in 4 types of cancers, including NSCLC ( 4 ), lung adenocarcinoma (LUAD) ( 28 , 29 ), lung cancer (LCA) ( 5 , 8 , 30 ), and Merkel cell carcinoma (MCC) (24627810). miR-873-5p is down-regulated in 14 types of cancers, including nasopharyngeal carcinoma (NPC) ( 32 ), lung cancer (LCA) ( 33 ), cervical cancer (CC) ( 34 , 35 ), EC ( 36 ), BC ( 10 , 11 , 37 , 38 ), pancreatic ductal adenocarcinoma (PDAC) ( 39 ), glioblastoma (GM) ( 9 , 40 – 42 ), osteosarcoma (OS) ( 43 ), papillary thyroid carcinoma(PTC) ( 44 ), CRC ( 6 , 45 – 49 ), esophageal cancer(ESCA) ( 50 ),GC ( 7 , 51 , 52 ), tongue squamous cell carcinoma (TSCC) ( 53 ), and pancreatic cancer(PC) ( 54 ).…”

Pan-cancer analysis identifies SEC11A as an immunological and prognostic biomarker

“…Forced expression of SEC11A may activate gastric cancer progression via promoting TGF-α secretion. Interestingly, study found that high SEC11A expression was examined in squamous cell carcinoma of tongue and its activation induced proliferation, migration, and invasion in tongue squamous cell carcinoma cells [7]. SEC11A up-regulation is in relation to increased cancer-specific mortality among esophageal squamous cell carcinoma [8].…”