miR-762 can negatively regulate menin in ovarian cancer

Hou, Rui; Yang, Zhuo; Wang, Shizhuo; Chu, Daming; Liu, Qifang; Liu, Jia; Luo, Jianlin

doi:10.2147/ott.s127872

Cited by 31 publications

(46 citation statements)

References 22 publications

(26 reference statements)

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“…MiR-762 has been reported to serve as oncogene in breast cancer and ovarian cancer 23,24 . To investigate whether miR-762 plays the similar roles in UCB, we first measured the level of miR-762 in UCB cells and 31 pairs of patient tissues.…”

Section: Mir-762 Is Overexpressed In Ucb Cells and Tissues And Facilmentioning

confidence: 99%

Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

et al. 2020

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Numerous evidences have shown that circular RNAs (circRNAs) play a key role in regulating the pathogenesis of cancer. However, the mechanism of circRNAs in urothelial carcinoma of bladder (UCB) remains largely unclear. In this study, we found circFAM114A2 was significantly downregulated both in UCB tissue specimens and cell lines, and the expression level was highly correlated with pathological TNM stage and grade. Functionally, overexpression of circFAM114A2 dramatically inhibited the migration, invasion and proliferation of UCB cells in vitro, and suppressed tumor growth in vivo. Mechanistically, we confirmed miR-762 was copiously pulled down by circFAM114A2 in 5637 and T24 cells. Fluorescence in situ hybridization (FISH) further indicated the cytoplasmic interactions between circFAM114A2 and miR-762. By using luciferase reporter assay, we found that miR-762 could directly target TP63. Subsequently, we found that circFAM114A2 might increase the expression of ΔNP63 (main isoform of TP63 in UCB) by sponging miR-762. Taken together, our results demonstrated that circFAM114A2 might serve as a competing endogenous RNA (ceRNA) of miR-762 in regulating the expression of ΔNP63, thus suppressed UCB progression through circFAM114A2/miR-762/ΔNP63 axis.

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Section: Mir-762 Is Overexpressed In Ucb Cells and Tissues And Facilmentioning

confidence: 99%

Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

et al. 2020

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“…miR-762 is upregulated in radiation-induced tumors in mice and may affect the pathways involved in apoptosis in this context 15 . Previous studies reported abnormal miRNA expression in numerous cancers, and miR-762 overexpression has been reported in breast cancer and ovarian cancer, where it promoted cancer cell growth and metastasis 16,17 . A previous study also demonstrated an association between miR-762 expression and oral carcinogenesis 18 .…”

Section: Discussionmentioning

confidence: 99%

“…In the future, further analysis of the regulation of circulating miR-762 on cancer promotion and progression in CRC patients will be advanced elucidated. Hou et al (2017) reported that miR-762 can downregulate the expression of a tumor suppressor protein menin through a binding site in its 3'-UTR and consequently upregulate the Wnt cell signaling pathway to promote the development of ovarian cancer 17 . Our present study rst revealed that circulating miR-762 expression may be useful as a biomarker for diagnosing CRC and higher serum miR-762 expressioncaused distant metastasis of patients with CRC.…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA-762 upregulates colorectal cancer might through Wnt-1/β-catenin signaling

Lai

Chang

Chen

et al. 2020

Preprint

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Background A number of microRNAs (miRNAs) have been demonstrated to be correlated with the diagnosis, progression and prognosis of colorectal cancer (CRC). However, the key miRNAs and the associated signaling pathways that regulate the growth and metastasis of CRC remain unclear. Methods miRNA array was analyzed in CRC CT26 cell-transplanted BALB/c mice. The effects of miR-762 mimics and inhibitors on the growth, invasion, cell cycle, and regulatory pathways of CRC CT26 cells were assessed. Cell cycle and sub-G1 assay were collected from the treated CT26 cells. Finally, blood samples were collected from patients with CRC. Further analysis to compare miR-762 levels in blood samples collected from CRC patients and normal control donors. Patients’ basic data were retrieved from electronic medical records and analyzed for age, gender, tumor staging, and survival. Results The miRNA levels in mice with CRC cell implantation indicated that plasma mmu-miR-762 was upregulated. Transfection of mmu-miR-762 mimic to CT26 cells increased cell viability, invasion, and EMT, whereas transfection of mmu-miR-762 inhibitor decreased the above abilities. Cells treated with high-concentration mmu-miR-762 inhibitor induced cell cycle arrest at G0/G1 phase. However, mmu-miR-762 did not cause apoptosis of cells. Western blot analysis showed that mmu-miR-762 mimic transfection upregulated the expression of Wnt-1 and β-catenin, as well as increased the nuclear translocation of β-catenin. Further analysis was performed to demonstrate the correlation of has-miR-762 with CRC, and blood samples were collected from CRC patients and control donors. The results showed that serum has-miR-762 levels in CRC patients were higher than in control donors. Among the CRC patients, patients with distant metastasis showed higher serum has-miR-762 levels than patients without distant metastasis. Conclusions The present study demonstrated that circulating miR-762 might be a biomarker with upregulation of CRC cell growth and invasion through the Wnt/β-catenin signaling.

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“…miR-762 is upregulated in radiation-induced tumors in mice and may affect the pathways involved in apoptosis in this context 15 . Several studies reported abnormal miRNA expression in numerous cancers, and miR-762 overexpression has been reported in breast cancer and ovarian cancer, where it promoted cancer cell growth and metastasis 16,17 . A previous study also demonstrated an association between miR-762 expression and oral carcinogenesis 18 To explore the molecular mechanisms by which miR-762 enhances CRC cell growth and invasion, Wnt/b-catenin was identified as a direct target of miR-762 in CRC cells.…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA-762 promotes colorectal cancer proliferation and invasion by upregulating the Wnt-1/β-catenin pathway

Lai

Chang

Chen

et al. 2019

Preprint

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Background: A number of microRNAs (miRNAs) have been demonstrated to be associated with the diagnosis, progression and prognosis of colorectal cancer (CRC). However, the function of miRNA-762 (miR-762) in CRC remains unclear, and the molecular mechanisms underlying the effects of miR‑762 in CRC require further investigation. Methods: The circulating miRNAs from BALB/c mice with CRC CT26 cell implantation were assayed by microarray. Then, miR-762 mimic and inhibitor were transfected to CT26 cells for analysis of cell viability, invasion, and epithelial-mesenchymal transition (EMT), cell cycle, and regulatory molecule expression. Human subjects were included for comparison the circulating miR-762 levels in CRC patients and control donors, as well as the patients with and without distant metastasis. Results: The screening for miRNA levels in mice with CRC cell implantation indicated that plasma miR-762 was upregulated. Transfection of miR-762 mimic to CT26 cells increased cell viability, invasion, and EMT, whereas transfection of miR-762 inhibitor decreased the above abilities. Western blot analysis showed that miR-762 mimic transfection upregulated the expression of Wnt-1 and b-catenin, as well as increased the nuclear translocation of b-catenin. Further analysis showed that serum miR-762 levels in CRC patients were higher than in control donors. Among the CRC patients (n = 20), six patients with distant metastasis showed higher serum miR-762 levels than the patients without distant metastasis. Conclusions: Circulating miR-762 could promote CRC disease development and progression through the Wnt/b-catenin signaling. miR-762 might be used as a biomarker for CRC diagnosis and targeted therapy.

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miR-762 can negatively regulate menin in ovarian cancer

Cited by 31 publications

References 22 publications

Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762

Circulating microRNA-762 upregulates colorectal cancer might through Wnt-1/β-catenin signaling

Circulating microRNA-762 promotes colorectal cancer proliferation and invasion by upregulating the Wnt-1/β-catenin pathway

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