miR-7/TGF-β2 axis sustains acidic tumor microenvironment-induced lung cancer metastasis

Su, Tao; Huang, Suchao; Zhang, Yanmin; Guo, Yajuan; Zhang, Shuwei; Guan, Jiaji; Meng, Mingjing; Liu, Linxin; Wang, Caiyan; Yu, Dihua; Kwan, Hiu Yee; Huang, Zhiying; Huang, Qiuju; Leung, Elaine Lai‐Han; Hu, Ming; Wang, Ying; Liu, Zhongqiu; Lu, Linlin

doi:10.1016/j.apsb.2021.06.009

Cited by 16 publications

(8 citation statements)

References 38 publications

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“…In breast cancer, its expression depends on estrogen ( 26 ). It was found that miR-7 is a new and reliable biomarker of an acidic tumor microenvironment (TME) and a novel therapeutic target for non-small cell lung cancer (NSCLC) ( 27 ). Ku et al found that LINC00240 acts as a sponge for miR-7-5p and induces EGFR overexpression.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of the miR-7/BCL2L1/P53 signaling axis regulating the progression of hepatocellular carcinoma

Zhang¹,

Wang²,

Zhu³

et al. 2023

Ann Transl Med

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Background: To investigate the roles of miR-7 and its potential mechanisms in hepatocellular carcinoma (HCC).Methods: The functions of miR-7 were identified and measured by 5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide], colony formation, transwell, and flow cytometry assays. A luciferase assay was applied to verify the direct binding of miR-7 on BCL2L1 3'untranslated region (3'UTR).An in vitro experiment was then used to investigate the biological effects of miR-7 and BCL2L1. A coimmunoprecipitation (COIP) assay was used to detect the protein interaction between BCL2L1 and P53.Results: We found that miR-7 overexpression suppressed cell proliferation, migration, and invasion in HCC. BCL2L1 was also demonstrated as a direct target gene of miR-7. This study showed that BCL2L1 could partially rescue the inhibitory effect of miR-7 on the proliferation, migration, and invasion of HCC cells. Our research showed that miR-7 could inhibit the epithelial-mesenchymal transition (EMT) pathway by regulating BCL2L1. We also further confirmed that miR-7 inhibits the proliferation, migration, and invasion of Hep3B and Huh7 cells by targeting BCL2L1. Furthermore, we observed that the BCL2L1 protein interacts with the P53 protein and BCL2L1 affects the development of liver cancer through P53. We also found that BCL2L1 could promote the invasion and migration of liver cancer cells through P53 inhibition.BCL2L1 also inhibited the expression of Caspase 3/7 in hepatoma cells by inhibiting the expression of P53.Conclusions: Our study demonstrated that miR-7/BCL2L1/P53 may serve as a regulatory molecular axis for HCC treatment. Our results suggest that miR-7/BCL2L1/P53 may have predictive value and represent a new treatment strategy for liver cancer.

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Section: Discussionmentioning

confidence: 99%

Molecular mechanism of the miR-7/BCL2L1/P53 signaling axis regulating the progression of hepatocellular carcinoma

Zhang¹,

Wang²,

Zhu³

et al. 2023

Ann Transl Med

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“…Tumor cells highly express many receptors/transporters specifically, and thus nanoparticles (NPs) engineered with targeting ligands can enter these cells selectively [ 22 ]. In addition, the tumor microenvironment (TME) has some specific characteristics, such as being weakly acidic, hypoxia, and so on [ 23 , 24 ]. These properties can be applied to the design of TME-responsive NPs that promote the precise uptake and release of drugs in tumor cells [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Nano-Based Drug Delivery of Polyphenolic Compounds for Cancer Treatment: Progress, Opportunities, and Challenges

Jia

Zhou

et al. 2023

Pharmaceuticals

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Polyphenols and their derivates, a kind of natural product distributed in herb plants, vegetables, and fruits, are the most abundant antioxidants in the human diet and have been found to display cancer-preventative effects in several epidemiological studies. The scientific community has also validated the anti-cancer bioactivities and low toxicities of polyphenolic compounds, including flavones, tannins, phenolic acids, and anthocyanins, through in vitro and in vivo studies. However, the low stability, weak targeting ability, poor solubility, and low bioavailability of pure polyphenolic agents have significantly impaired their treatment efficacy. Nowadays, nano-based technology has been applied to surmount these restrictions and maximize the treatment efficacy of polyphenols. In this review, we summarize the advantages and related mechanisms of polyphenols in cancer treatment. Moreover, aiming at the poor solubility and low bioavailability of pure polyphenols in vivo, the advantages of nano-based delivery systems and recent research developments are highlighted. Herein, particular emphasis is mainly placed on the most widely used nanomaterials in the delivery of natural products, including liposomes, micelles, and nanogels. Finally, we present an overview and the challenges of future implementations of nano-based delivery systems of polyphenolic compounds in the cancer therapeutic field.

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“…It can inhibit cell proliferation, migration, and invasion in NSCLC cells by targeting FAK through the ERK/MAPK signaling pathway (Cao et al 2016 ). The miR-7/TGF-b2 axis sustains lung cancer metastasis induced by the acidic tumor microenvironment (Su et al 2022 ). In addition, upregulation of miR-7 by EGFR through the Ras/ERK/Myc signaling promotes lung tumorigenesis (Chou et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

EIF4A3-induced circular RNA SCAP facilitates tumorigenesis and progression of non-small-cell lung cancer via miR-7/SMAD2 signaling

Zhang

2023

Environ Sci Pollut Res

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The eukaryotic translation initiation factor 4A (eIF4A) family determines transcription efficiency by directly binding to precursor RNAs. One member, EIF4A3, modulates the expression of circRNAs. Circular RNA SCAP (circSCAP), a newly found circRNA, has been implicated in atherosclerosis. Yet, how circSCAP regulates cancer development and progression remains understudied. Here, we investigated the function of circSCAP and the molecular mechanism in the tumorigenesis and progression of non-small-cell lung cancer (NSCLC). CircSCAP was upregulated in both NSCLC tissues and cell lines and was mainly located in the cytoplasm. CircSCAP expression was promoted by EIF4A3, which was associated with poor prognosis in patients with NSCLC. CircSCAP sponged miR-7 to upregulate small mothers against decapentaplegic 2 (SMAD2). CircSCAP knockdown undermined cell proliferation, migration, and invasion abilities in NSCLC cell lines (SPCA1 and A549), which was rescued by either inhibiting miR-7 or overexpressing SMAD2. Moreover, circSCAP knockdown upregulated E-cadherin, while downregulating N-cadherin, Vimentin, and MMP9 in SPCA1 and A549 cells, which were abolished by either inhibiting miR-7 or overexpressing SMAD2. Additionally, miR-7 was markedly downregulated, whereas SMAD2 was significantly upregulated in NSCLC tissues. MiR-7 expression was inversely correlated with circSCAP and SMAD2 expression in NSCLC tissues. In conclusion, this study demonstrates that circSCAP is significantly upregulated in NSCLC cell lines and tissues and elucidates that circSCAP facilitates NSCLC progression by sponging miR-7 and upregulating SMAD2. The study provides a novel molecular target for early diagnosis and treatment of NSCLC.

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miR-7/TGF-β2 axis sustains acidic tumor microenvironment-induced lung cancer metastasis

Cited by 16 publications

References 38 publications

Molecular mechanism of the miR-7/BCL2L1/P53 signaling axis regulating the progression of hepatocellular carcinoma

Molecular mechanism of the miR-7/BCL2L1/P53 signaling axis regulating the progression of hepatocellular carcinoma

Nano-Based Drug Delivery of Polyphenolic Compounds for Cancer Treatment: Progress, Opportunities, and Challenges

EIF4A3-induced circular RNA SCAP facilitates tumorigenesis and progression of non-small-cell lung cancer via miR-7/SMAD2 signaling

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