miR-675 Mediates Downregulation of Twist1 and Rb in AFP-Secreting Hepatocellular Carcinoma

Hernandez, Jonathan M.; Elahi, Abul; Clark, Charles W.; Wang, J.; Humphries, Leigh Ann; Centeno, B.; Bloom, Gregory; Fuchs, Bryan C.; Yeatman, Tim; Shibata, David

doi:10.1245/s10434-013-3106-3

Cited by 70 publications

(66 citation statements)

References 38 publications

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“…In addition, the overexpression of miR-675 alters cellular morphology, reduces invasive potential, and increases anchorageindependent growth capacity. These findings are consistent with a mesenchymal-to-epithelial transition associated with a reduction in the expression of the key EMT mediator, Twist1 (Hernandez et al, 2013).…”

Section: Mir-675supporting

confidence: 83%

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

Bronte

Fanale

et al. 2016

Critical Reviews in Oncology/Hematology

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Section: Mir-675supporting

confidence: 83%

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

Bronte

Fanale

et al. 2016

Critical Reviews in Oncology/Hematology

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“…H19/ miR-675 functions in an indirect way by targeting important downstream genes. Many targets of miR-675 have been identified, such as Twist1 and RB in hepatocellular carcinoma and colorectal cancer [27,28], CaMKIIδ in cardiomyocyte hypertrophy [29], RUNX1 and p53 in bladder cancer [30], and TGF-β1/Smad3 in osteogenic differentiation [37]. We show that CRYAA, which encodes the predominant structural protein involved in the maintenance of lens clarity and refractive properties, is a new target of miR-675 in HLECs and is involved in the pathogenesis of nuclear ARC.…”

Section: Discussionmentioning

confidence: 99%

“…Many targets of miR-675 have been identified, such as Twist1 and RB in hepatocellular carcinoma and colorectal cancer [27,28]; CaMKIIδ in cardiomyocyte hypertrophy [29]; RUNX1 and p53 in bladder cancer [30]; and TGF-β1/Smad3 in osteogenic differentiation [31]. We thus further investigate the expression level of the above-mentioned target genes between nuclear ARC and transparent lens capsules by qRT-PCR.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Liu

Shan

et al. 2018

Cell Physiol Biochem

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Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.

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“…However, H19 is found to reverse EMT by activating the miR-200 family in hepatocellular carcinoma (35). In addition, overexpressed miR-675 downregulates Twist1 and reverses EMT in AFP-secreting hepatocellular carcinoma (30). Furthermore, no effect of H19 reduction on proliferation is observed in endometrial cancer cells.…”

Section: Discussionmentioning

confidence: 93%

“…The observations in certain cancers support an oncogenic role of H19, since it is overexpressed and regulates genes involved in tumor growth, metastasis and angiogenesis (15,24,28,29). However, in other cases, H19 was not considered an oncodevelopmental marker (13,30). At present, the function of H19 in endometrial cancer invasion has not been well established.…”

Section: Discussionmentioning

confidence: 99%

H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition

Zhao

Chen

et al. 2016

Oncology Letters

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Abstract. Endometrial cancer is one of the most common types of gynecological malignancy worldwide. Novel biomarkers and therapeutic targets are imperative for improving patients' survival. Previous studies have suggested the long non-coding RNA H19 as a potential cancer biomarker. To investigate the role of H19 in endometrial cancer, the present study examined the expression pattern of H19 in endometrial cancer tissues by quantitative polymerase chain reaction, and characterized its function in the endometrial cancer cell line via knocking down its expression with small interfering RNAs. It was found that H19 level was significantly higher in tumor tissues than in paratumoral tissues. Knockdown of H19 did not affect the growth rate of HEC-1-B endometrial cancer cells, but significantly suppressed in vitro migration and invasion of HEC-1-B cells. Furthermore, H19 downregulation decreased Snail level and increased E-cadherin expression without affecting vimentin level, indicating partial reversion of epithelial-mesenchymal transition (EMT). The present findings suggested that H19 contributed to the aggressiveness of endometrial cancer by modulating EMT process.

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miR-675 Mediates Downregulation of Twist1 and Rb in AFP-Secreting Hepatocellular Carcinoma

Abstract: Expression of the miR-675 in hepatocellular carcinoma links a dramatic upregulation of proliferative and growth capacity with inhibition of motility in HCC cells.

Cited by 70 publications

References 38 publications

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition

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