miR-618 Inhibits Prostate Cancer Migration and Invasion by Targeting FOXP2

Song, Xiaolei; Tang, Yao; Lei, Xiang-Hui; Zhao, Shan‐Chao; Wu, Ziqing

doi:10.7150/jca.17407

Cited by 35 publications

(50 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…qRT-PCR and dual luciferase reporter analysis further demonstrated that circ_0005230 directly sponges miR-618 and inhibits miR-618 expression. Previously, Song et al revealed that miR-618 could inhibit prostate cancer metastatic properties through targeting FOXP2 [22]. In addition, miR-618 regulates cell proliferation by targeting PI3K/ Akt signaling in thyroid cancer [23].…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Circular RNAs (circRNAs) are a class of non-coding RNAs. They have been proved to be critically involved in tumorigenesis and progression of malignancies through competing endogenous RNA (ceRNA) mechanism. Nevertheless, the exploration between circRNAs and pathogenesis of breast cancer (BC) is limited. Previously, circ_0005230 was identified upregulated in BC tissues screened by circRNA microarray. In the present study, we aimed to investigate the expression pattern, functional role, and mechanism of circ_0005230 in BC. Methods: qRT-PCR was conducted to elucidate the expression levels of circ_0005230 in BC tissues and cells. Additionally, the clinical severity and prognostic value were investigated. CCK-8, colony-forming, flow cytometric assays were performed. Animal study was conducted to validate the in vitro data. What’s more, Transwell assays were induced to detect the cell metastatic properties of circ_0005230 exerts in BC cells. Luciferase reporter assay was used to measure the mechanism of circ_0005230. Results: circ_0005230 was overexpressed in BC tissue specimens and cell lines. The overexpression of circ_0005230 was related to adverse phenotypes in the patients with BC. In addition, circ_0005230 could be regarded as a prognostic predictor in BC patients. In vitro and in vivo data demonstrated the cell growth promoting role of circ_0005230. Moreover, circ_0005230 could also promote cell migratory and invasive capacities. For the mechanism investigation, circ_0005230 was proved to be a sponge of miR-618, and expression of miR-618 could regulate CBX8 expression via targeting the 3’UTR of CBX8. Rescue assays also illustrated an oncogenic function of circ_0005230 in BC via acting as a miR-618 sponge to promote CBX8 expression. Conclusion: circ_0005230/miR-618/CBX8 axis might play a key role in BC tumorigenesis and development.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Interestingly, these authors also con rmed these results in vivo in a nude mouse model [17]. Furthermore, it should be noted that FOXP2 has also been associated with the promotion of migration and invasion of tumor cellsin breast cancer [26,27], hepatocellular carcinoma [14], prostate cancer [10] and colorectal carcinoma [28], among others. Therefore, results from in vitro studies suggest that FOXP2 may promote the malignant biological behavior of glioma cells.…”

Section: Foxp2 Expression In Glioblastomamentioning

confidence: 68%

“…The expression of FOXP2 in some tumors has previously been reported. On the one hand, high expression of FOXP2 has been associated with a bad prognosis in prostate cancer [10], neuroblastoma [11] and multiple mieloma [12]. On the other hand, other studies have reported an association between FOXP2 expression and a better prognosis in breast cancer [13], liver cell carcinoma [14], osteosarcoma [15] and gastric cancer [16].…”

Section: Introductionmentioning

confidence: 99%

Low Expression of FOXP2 is Associated with Better Prognosis in Glioblastoma.

Plata‐Bello

Fariña-Jerónimo

Betancor

2020

Preprint

View full text Add to dashboard Cite

FOXP2 expression has been associated with the prognosis of some tumors, but the role of FOXP2 in glioblastoma has not been studied in-depth until now. The aim of the present work is to study the role of FOXP2 as a prognostic biomarker in glioblastoma.This is a retrospective observational case series study in which the expression of FOXP2 has been analyzed both at the protein level (immunohistochemistry) and at the mRNA level (RNAseq, in a cohort of glioblastoma patients from The Cancer Genome Atlas [TCGA] database). Other molecular and clinical data have also been included in the study, with special focus on miRNA expression data.Survival analysis using Log-Rank test and COX-regression have been used. Non-parametric statistical tests were also used to study differences between low and high FOXP2 expression groups.Patients with a high expression of FOXP2 protein showed a worse prognosis than those patients with low expression in both, progression free survival (PFS) (HR=1.711; p=0.034) and overall survival (OS) (HR=1.809;p=0.014). These associations were still statistically significant in multivariate analysis.No prognostic association was found with FOXP2 RNA expression. Interestingly, two miRNAs that target FOXP2 (hsa-miR-181a-2-3p and hsa-miR-20a-3p) showed an interaction effect on OS with FOXP2 expression. A low level of these miRNAs expression was associated with a significantly worse prognosis in patients with high FOXP2 RNA expression.Higher expression of FOXP2 at the protein level is associated with a worse prognosis. This protein expression may be regulated by the expression of specific miRNAs that target FOXP2 mRNA: hsa-miR-181a-2-3p and hsa-miR-20a-3p.

show abstract

“…Numerous recent studies indicate a tumor suppressor like role for FOXP2 (Campbell et al, 2010;Cuiffo et al, 2014;Yan et al, 2015;Diao et al, 2018;Song et al, 2017;Li et al, 2019), others present evidence for an oncogene-like role of the protein (Campbell et al, 2010;Zhong et al, 2017;Wu et al, 2018;.…”

Section: Forkhead Box Protein P2 Encoded By Foxp2 Genementioning

confidence: 99%

Use of signals of positive and negative selection to distinguish cancer genes and passenger genes

Bányai

Trexler

Kerekes

et al. 2020

Preprint

View full text Add to dashboard Cite

A major goal of cancer genomics is to identify all genes that play critical roles in carcinogenesis. Most approaches focused on genes that are positively selected for mutations that drive carcinogenesis and neglected the role of negative selection. Some studies have actually concluded that negative selection has no role in cancer evolution. In the present work we have re-examined the role of negative selection in tumor evolution through the analysis of the patterns of somatic mutations affecting the coding sequences of human genes. Our analyses have confirmed that tumor suppressor genes are positively selected for inactivating mutations. Oncogenes, however, were found to display signals of both negative selection for inactivating mutations and positive selection for activating mutations. Significantly, we have identified numerous human genes that show signs of strong negative selection during tumor evolution, suggesting that their functional integrity is essential for the growth and survival of tumor cells.

show abstract

miR-618 Inhibits Prostate Cancer Migration and Invasion by Targeting FOXP2

Cited by 35 publications

References 38 publications

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Low Expression of FOXP2 is Associated with Better Prognosis in Glioblastoma.

Use of signals of positive and negative selection to distinguish cancer genes and passenger genes

Contact Info

Product

Resources

About