MiR-613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5

Zhang, Yiting; Zhu, Xinyue; Zhu, Xiaomin; Wu, Yan; Liu, Yajun; Yao, Borui; Huang, Zhenping

doi:10.1177/1010428317691674

Cited by 39 publications

(32 citation statements)

References 28 publications

(35 reference statements)

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“…LncRNA DANCR is over‐expressed in osteosarcoma cell proliferation, migration, invasion, and xenograft tumor growth in vitro and in vivo and regulates osteosarcoma stemness features by upregulating AXL via miR‐33a‐5p inhibition (Jiang et al, ). Besides, Zhang, Zhu et al () have reported that miR‐613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5, which is closely correlated with our study.…”

Section: Discussionsupporting

confidence: 90%

Long noncoding RNA DANCR aggravates retinoblastoma through miR‐34c and miR‐613 by targeting MMP‐9

Wang

Yang

2018

Journal Cellular Physiology

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Long non-coding RNAs (lncRNAs) have been identified to play vital roles in cancers, including human retinoblastoma (RB). However, the deepgoing mechanism is still ambiguous. In present study, we investigate the biological role of lncRNA DANCR (differentiation antagonizing non-protein coding RNA) in carcinogenesis of RB. Results revealed that DANCR was up-regulated in RB tissue and cell lines. Moreover, the ectopic overexpression of DANCR indicated poor overall survivals and disease free survival (DFS) for RB patients. In vitro and in vivo experiments, DANCR knockdown suppress the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) correlated protein (N-cadherin, Vimentin) of RB cells. Bioinformatics analysis predicted that miR-34c and miR-613 targeted with 3'-UTR of DANCR, besides, miR-34c and miR-613 also targeted with 3'-UTR of MMP-9, which was validated by luciferase reporter assay. Functional experiments demonstrated that miR-34c and miR-613 could reverse the oncogenic function of DANCR in RB tumorigenesis. In conclusion, our results reveal that DANCR function as competing endogenous RNA (ceRNA) for miR-34c and miR-613 to modulate progression and metastasis in RB oncogenesis via targeting MMP-9, presenting the in-depth regulation of DANCR in RB and providing a novel insight for ceRNA mechanism for RB.

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Section: Discussionsupporting

confidence: 90%

Long noncoding RNA DANCR aggravates retinoblastoma through miR‐34c and miR‐613 by targeting MMP‐9

Wang

Yang

2018

Journal Cellular Physiology

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“…Besides that, E2F5 had been verified as a direct target of several miRNAs and had negative association with their expression, such as miR-129-3p [36], miR-132 [37] and miR-613 [38]. In present research, miR-1271-5p was found to directly target E2F5 and was inversely regulated its expression in OC.…”

Section: Discussionsupporting

confidence: 55%

MicroRNA-1271-5p inhibits the tumorigenesis of ovarian cancer through targeting E2F5 and negatively regulates the mTOR signaling pathway

Li¹,

Shi²,

Xu³

2020

Preprint

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Background: MicroRNA-1271-5p (miR-1271-5p) has been reported to participate in the progression of many human cancers. However, the role of miR-1271-5p still remains unclear in ovarian cancer (OC). Therefore, we explored the effect of miR-1271-5p on the development of OC in present study. Methods: We measured the miR-1271-5p expression via the qRT-PCR assay. Then the function of miR-1271-5p was analyzed through MTT and Transwell assays. The relationship among miR-1271-5p and E2F5 was verified by dual luciferase assay. The protein expression levels were examined through western blot.Results: MiR-1271-5p was downregulated in OC tissues which predicted poor prognosis of OC patients. Moreover, E2F5 was a direct target of miR-1271-5p in OC. And miR-1271-5p suppressed cell proliferation, migration and invasion in OC through targeting E2F5. Furthermore, E2F5 was upregulated in OC tissues which predicted poor prognosis of OC patients. Besides that, miR-1271-5p suppressed EMT and mTOR pathway in OC. Conclusion: MiR-1271-5p inhibited the tumorigenesis of OC through targeting E2F5 and negatively regulated the mTOR signaling pathway.

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“…12 Recently, abnormally expressed miRNAs were broadly implicated in retinoblastoma development. [13][14][15] However, the biological role of miR-214-3p in retinoblastoma is still largely unclear. In this study, miRNA array analysis revealed that miR-214-3p was significantly down-regulated in retinoblastoma tissues.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-214-3p Regulates Multi-Drug Resistance and Apoptosis in Retinoblastoma Cells by Targeting ABCB1 and XIAP</p>

Yang¹,

Zhang²,

Lu³

et al. 2020

OTT

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Background: MicroRNAs (miRNAs) have been shown to contribute to the initiation and progression of human cancer, including retinoblastoma. However, expression levels and potential roles of miRNAs in retinoblastoma remain largely unknown. In this study, we aimed to identify dysregulated miRNAs and explore their functional roles in the development of retinoblastoma. Material and Methods: First, miRNA expression profiling in retinoblastoma tissues was performed via microarray analysis. To evaluate the involvement of miR-214-3p in multi-drug resistance, gain-of-function experiments were employed in vitro and in vivo. Bioinformatics analysis, luciferase reporter assay, qRT-PCR and Western blot were used to investigate the underlying mechanisms. Results: Here, we identified 57 up-regulated and 34 down-regulated miRNAs. Among them, miR-214-3p was the most significantly decreased. We found that miR-214-3p level was positively correlated with clinical outcome and chemotherapy response. Overexpression of miR-214-3p significantly sensitized retinoblastoma cells to multiple chemodrugs and promoted cell apoptosis in vitro and in vivo. Further investigations revealed that miR-214-3p directly regulated ABCB1 and XIAP expression through interacting with the 3' untranslated regions (3'UTRs). Pearson correlation analysis showed that miR-214-3p expression in retinoblastoma tissues was negatively correlated with ABCB1 and XIAP expression. We also observed that overexpression of ABCB1 or XIAP partly reversed the chemoresistance inhibition-induced by miR-214-3p overexpression. Conclusion: Our data demonstrate that miR-214-3p functions as a tumor suppressor to inhibit the chemoresistance in retinoblastoma, suggesting that miR-214-3p might be potential diagnostic and therapeutic targets for retinoblastoma treatment.

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MiR-613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5

Cited by 39 publications

References 28 publications

Long noncoding RNA DANCR aggravates retinoblastoma through miR‐34c and miR‐613 by targeting MMP‐9

Long noncoding RNA DANCR aggravates retinoblastoma through miR‐34c and miR‐613 by targeting MMP‐9

MicroRNA-1271-5p inhibits the tumorigenesis of ovarian cancer through targeting E2F5 and negatively regulates the mTOR signaling pathway

<p>miR-214-3p Regulates Multi-Drug Resistance and Apoptosis in Retinoblastoma Cells by Targeting ABCB1 and XIAP</p>

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