MiR-608 rs4919510 C &gt; G polymorphism increased the risk of bladder cancer in an Iranian population

Hashemi, Mohammad; Bizhani, Fatemeh; Danesh, Hiva; Narouie, Behzad; Sotoudeh, Mehdi; Radfar, Mohammad Hadi; Ramezani, Mehdi; Bahari, Gholamreza; Taheri, Mohsen; Saeid, Ghavami

doi:10.3934/genet.2016.4.212

Cited by 7 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-608 is a novel prognostic marker in carcinogenesis with its expression downregulated in various cancers including chordoma (18), colon cancer (19), glioblastoma (20) and osteosarcoma (21). Single-nucleotide polymorphisms in miR-608 have also been associated with several cancers such as nasopharyngeal carcinoma (39), colorectal adenocarcinoma (40)(41)(42), breast (43,44) and bladder cancer (45). In recent years evidence has emerged illustrating the role miR-608 plays as a tumour suppressor.…”

Section: Discussionmentioning

confidence: 99%

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Othman

Nagoor

2017

International Journal of Oncology

View full text Add to dashboard Cite

Lung cancer remains a major health problem with a low 5-year survival rate of patients. Recent studies have shown that dysregulation of microRNAs (miRNAs) are prevalent in lung cancer and these aberrations play a significant role in the progression of tumour progression. In the present study, bioinformatics analyses was employed to predict potential miR-608 targets, which are associated with signaling pathways involved in cancer. Luciferase reporter assay identified AKT2 as a novel target of miR-608, and suppression of its protein levels was validated through western blot analysis. Zebrafish embryos were microinjected with cells transfected with miR-608 to elucidate the role of miR-608 in vivo, and immunostained with antibodies to detect activated caspase-3. We present the first evidence that miR-608 behaves as a tumour suppressor in A549 and SK-LU-1 cells through the regulation of AKT2, suggesting that selective targeting of AKT2 via miR-608 may be developed as a potential therapeutic strategy for miRNA-based non-small cell lung cancer (NSCLC) therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Othman

Nagoor

2017

International Journal of Oncology

View full text Add to dashboard Cite

show abstract

“…A total of 136 patients with histopathologically confirmed papillary urothelial cancers of the bladder and 144 healthy controls were enrolled in this case-control study. The study design and recruitment procedures were described previously [35] . All participants were from the Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.…”

Section: Methodsmentioning

confidence: 99%

Association between Pri-miR-34b/c rs4938723 polymorphism and bladder cancer risk

Hashemi¹,

Hasanpour²,

Danesh³

et al. 2019

J Biomed Res

View full text Add to dashboard Cite

Several studies examined the impact of miR-34b/c rs4938723 polymorphism and cancer risk, but the findings are inconsistent. However, no study has been conducted to inspect the impact of miR-34b/c polymorphism on bladder cancer. This study aimed to assess possible association between rs4938723 polymorphism and bladder cancer risk. This case-control study was done on 136 pathologically proven bladder cancer patients and 144 controls. Genotyping of Pri-miR-34b/c rs4938723 polymorphism was achieved by using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Our findings did not show any statistically significant differences in genotype and allele frequencies between bladder cancer and controls. Larger sample sizes with diverse ethnicities are required to validate our findings.

show abstract

“…A study by Shen et al (33) demonstrated that the miR-3131 expression level was upregulated by 92-fold in HepG2 cells treated with Ganoderma lucidum polysaccharide, proposing that miR-3131 may be involved in the proliferation and differentiation of HCC cells. Polymorphisms in miR genes, including pri-miR (17,34,35), pre-miR (36) and mature miR, may affect the processing of miR as well as the regulatory function on their target genes, and consequently may be implicated in the development and prognosis of various types of cancer (37)(38)(39). In conclusion, to the best of our knowledge, the present study provided evidence for the first time that the 3-bp indel polymorphism of pre-miR-3131 significantly increased the risk of developing PCa in a sample of an Iranian population.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism

Hashemi

Bahari

Sattarifard

et al. 2017

Molecular and Clinical Oncology

Self Cite

View full text Add to dashboard Cite

Abstract. The present study aimed to examine the impact of a 3-bp indel (rs57408770) polymorphism within the pre-microRNA (miR)-3131 polymorphism on prostate cancer (PCa) risk in a sample of an Iranian population. In total, 340 subjects, including 177 patients with PCa and 170 patients with benign prostatic hyperplasia, were enrolled in the present case-control study. A mismatch polymerase chain reaction-restriction fragment length polymorphism method was designed for genotyping the 3-bp indel (rs57408770) polymorphism. The present findings demonstrated that the indel variant significantly increased the risk of PCa in codominant [odds ratio (OR)=2.23, 95% confidence interval (CI)=1.13-4.37; P=0.021, insertion (ins)/ins vs. deletion (del)/del] and recessive (OR=2.33, 95% CI=1.25-4.36; P=0.009, ins/ins vs. del/del + del/ins). In conclusion, to the best of our knowledge, the present findings for the first time proposed that a 3-bp indel variant of miR-3131 may be a risk factor for susceptibility to PCa in a sample of an Iranian population. Further studies with different ethnicities and larger sample sizes are required to validate the present findings. IntroductionProstate cancer (PCa), the second most common malignancy in men, is the fifth leading cause of cancer-related mortality among men globally (1). The incidence rate of PCa in Iran is lower than that in the rest of the world (2-4). Despite the high prevalence of PCa, little is known about the mechanisms underlying the development and progression of PCa. It has been proposed that genomic and environmental factors contribute to the development and progression of PCa (5-8). Twin studies have indicated that 42% of the variation in PCa risk may be attributed to genetics (9). Single nucleotide polymorphisms (SNPs), the most common type of genetic variation in the human genome, have been demonstrated to be associated with the risk of developing PCa (10-12).MicroRNA (miR) are small, non-coding, endogenous, single-stranded RNA molecules that are ~22 nucleotides in length (13,14). They regulate gene expression by directing sequence-specific degradation or inhibiting translation of target mRNA (13,14). Mounting evidence has suggested that mutation or SNPs in miR genes may affect target-binding activity, expression, or processes of mature miR, thus affecting the expression of their target genes (15,16). Polymorphisms in mature and/or pre-miR sequences may affect miR biogenesis and be associated with the development of various types of cancer (17-21). Small insertions and deletion (indels) polymorphisms are one of the most common genetic alterations in the human genome that influence human traits and diseases (22,23). There is limited information regarding the association between pre-miR-3131 polymorphisms and cancer risk. Recently, Wang et al (20) investigated the impact of a 3-bp indel polymorphism (rs57408770) in pre-miR-3131 on hepatocellular carcinoma (HCC) and observed that the insertion (ins) allele significantly increased the risk of HCC in a Chinese population. ...

show abstract

MiR-608 rs4919510 C > G polymorphism increased the risk of bladder cancer in an Iranian population

Cited by 7 publications

References 42 publications

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

Association between Pri-miR-34b/c rs4938723 polymorphism and bladder cancer risk

Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism

Contact Info

Product

Resources

About