miR‐605‐5p promotes invasion and proliferation by targeting TNFAIP3 in non–small‐cell lung cancer

Liao, Youxia; Cao, Lirong; Wang, Fang; Rong, Pang

doi:10.1002/jcb.29323

Cited by 24 publications

(19 citation statements)

References 25 publications

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“…Invasion and metastasis are the main causes of poor prognosis and high mortality rate in patients with lung cancer (4). Although great efforts, such as the identification of novel biomarkers, establishment of modified operations and development of specific drugs, have been made in the clinical setting, the postoperative survival rate of patients has not significantly improved (5,6). Therefore, it is of great clinical significance to explore the molecular mechanisms underlying the progression of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non‑small cell lung cancer

Wang

Li²,

Zhang

2020

Mol Med Rep

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The platelet isoform of phosphofructokinase (PFKP) is a rate-limiting enzyme involved in glycolysis that serves an important role in various types of cancer. The aim of the present study was to explore the specific regulatory relationship between PFKP and non-small cell lung cancer (NSCLC) progression. PFKP expression in NSCLC tissues and corresponding adjacent tissues was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical analysis. PFKP expression in human bronchial epithelial cells (16HBE) and NSCLC cells (H1299, H23 and A549) was also detected using RT-qPCR. Cell proliferation was detected by Cell Counting Kit-8 and colony formation assays. Transwell invasion and wound healing assays, and flow cytometry were used to detect cell invasion, migration and apoptosis, respectively. The expression levels of glycolysis-associated enzymes (hexokinase-2, lactate dehydrogenase A and glucose transporter-1), epithelial-mesenchymal transition-related proteins (N-cadherin, vimentin and E-cadherin) and apoptosis-related proteins (caspase-3 and B-cell lymphoma-2) were detected by western blotting. Glucose uptake, lactate production and the adenosine trisphosphate/adenosine diphosphate ratio were measured using the corresponding kits. The results of the present study demonstrated that PFKP expression was upregulated in NSCLC tissues and cells, and PFKP expression was related to lymph node metastasis and histological grade. In addition, overexpression of PFKP inhibited cell apoptosis, and promoted proliferation, migration, invasion and glycolysis of H1299 cells, whereas knockdown of PFKP had the opposite effects. In conclusion, PFKP inhibited cell apoptosis, and promoted proliferation, migration, invasion and glycolysis of NSCLC cells; these findings may lay the foundation for novel treatments of NSCLC.

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Section: Introductionmentioning

confidence: 99%

Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non‑small cell lung cancer

Wang

Li²,

Zhang

2020

Mol Med Rep

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“…However, as far as we know, there are limited studies towards the identified miRNAs in HPV-signature. A research implied that miR-605-5p promoted proliferation and invasion of non-small-cell lung cancer cell carcinoma via sponging TNFAIP3 ( 48 ). It was reported miR-135b-3p could inhibit cell clonogenicity and metastasis in triple-negative breast cancer by targeting RGMA ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Distinguishable Prognostic miRNA Signatures of Head and Neck Squamous Cell Cancer With or Without HPV Infection

et al. 2021

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Since their discovery in the 1990’s, microRNAs (miRNA) have opened up new vistas in the field of cancer biology and are found to have fundamental roles in tumorigenesis and progression. As head and neck squamous cell carcinoma (HNSCC) with positive human papillomavirus (HPV+) is significantly distinct from its HPV negative (HPV−) counterpart in terms of both molecular mechanisms and clinical prognosis, the current study aimed to separately develop miRNA signatures for HPV+ and HPV− HNSCC as well as to explore the potential functions. Both signatures were reliable for the prediction of prognosis in their respective groups. Then Enrichment analysis was performed to predict the potential biological functions of the signatures. Importantly, combining previous studies and our results, we speculated that HPV+ HNSCC patients with low signature score had better immunity against the tumors and enhanced the sensitivity of therapies leading to improved prognosis, while HPV− HNSCC patients with high signature score acquired resistance to therapeutic approaches as well as dysregulation of cell metabolism leading to poor prognosis. Hence, we believe that the identified signatures respectively for HPV+ and HPV− HNSCC, are of great significance in accessing patient outcomes as well as uncovering new biomarkers and therapeutic targets, which are worth further investigation through molecular biology experiments.

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“…miR-605 also functions as a tumor suppressor in melanoma by regulating the expression of inositol polyphosphate-4-phosphatase type II B ( 35 ). In addition, miR-605 was also demonstrated to affect the proliferation, migration and invasion of non-small cell lung cancer, prostate cancer, and breast cancer ( 18 , 19 , 36 ). In the present study, miR-605 was found to be a vital factor in the progression of OS, as the downregulation of miR-605 significantly promoted cell proliferation, migration, and invasion of OS, which indicates that miR-605 may be involved in the progression and development of OS.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the expression and function of miR-605 were investigated in OS tissues and cells in vitro, however, the role of miR-605 in the progression and prognosis of OS should be verified in in vivo experiments. On the other hand, previous studies have provided some potential targets of miR-605, such as tumor necrosis factor α-induced protein 3, EN2, enhancer of zeste 2 (EZH2) and forkhead Box P1, which mediate the functional effects of miR-605 in human malignancies ( 18 , 19 , 37 , 38 ). EZH2 is a widely investigated oncogene in several human malignancies, such as non-small cell lung cancer and bladder cancer ( 39 , 40 ).…”

Section: Discussionmentioning

confidence: 99%

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Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

2020

Oncol Lett

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Osteosarcoma (OS) is a type of primary bone tumor, which is one of the leading causes of cancer-related death. MicroRNA (miR)-605 has been demonstrated to act as a prognostic biomarker and therapeutic target in various cancers, such as breast cancer and non-small cell lung cancer, but its function in OS remains unclear. The aim of the present study was to investigate the prognostic value of miR-605 in patients with OS by evaluating its expression levels and to explore the biological function of miR-605 in OS progression. For this purpose, tumor tissues and adjacent normal tissues were collected from OS patients, and the expression of miR-605 in the collected tissues and OS MG63, U2OS, HOS, and SAOS-2 cell lines was detected by quantitative real-time PCR. The prognostic value of miR-605 was evaluated by Kaplan-Meier survival curves and Cox regression analysis. The effects of miR-605 on OS cell proliferation, migration and invasion were analyzed by the CCK-8 and transwell assays, respectively. The results of the present study revealed that miR-605 was significantly downregulated in OS tissues compared with adjacent normal tissues, which was associated with the clinical stage and distant metastasis of patients. Additionally, the downregulation of miR-605 predicted the poor prognosis of patients with OS and served as an independent prognostic indicator. The downregulation of miR-605 enhanced cell proliferation, migration, and invasion of OS cells, which suggested that miR-605 may be involved in the progression of OS. The findings of the present study provide a new therapeutic target for the treatment of patients with OS.

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miR‐605‐5p promotes invasion and proliferation by targeting TNFAIP3 in non–small‐cell lung cancer

Cited by 24 publications

References 25 publications

Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non‑small cell lung cancer

Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non‑small cell lung cancer

Distinguishable Prognostic miRNA Signatures of Head and Neck Squamous Cell Cancer With or Without HPV Infection

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

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