MiR-590-3p suppresses hepatocellular carcinoma growth by targeting TEAD1

Gong, Lan

doi:10.1177/1010428317695947

Cited by 45 publications

(44 citation statements)

References 24 publications

(29 reference statements)

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“…31 In hepatocellular carcinoma cells, miR-590-3p suppresses cell growth in vitro and in vivo by targeting TEAD1. 33 In intrahepatic cholangiocarcinoma cells, miR-590-3p dramatically suppresses cell migration and invasion. 32 In breast cancer cells, miR-590-3p suppresses cell survival and triggers cell apoptosis via targeting sirtuin-1 and deacetylation of p53.…”

Section: Discussionmentioning

confidence: 99%

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

Yuan

Pan

Wen

et al. 2019

J Cellular Molecular Medi

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Previous studies have implicated the attractive and promising role of miR‐590‐3p to restore the cardiac function following myocardial infarction (MI). However, the molecular mechanisms for how miR‐590‐3p involves in cardiac fibrosis remain largely unexplored. Using human cardiac fibroblasts (HCFs) as the cellular model, luciferase report assay, mutation, EdU assay and transwell migration assay were applied to investigate the biological effects of miR‐590‐3p on the proliferation, differentiation, migration and collagen synthesis of cardiac fibroblasts. We found that miR‐590‐3p significantly suppressed cell proliferation and migration of HCFs. The mRNA and protein expression levels of α‐SMA, Col1A1 and Col3A were significantly decreased by miR‐590‐3p. Moreover, miR‐590‐3p directly targeted at the 3’UTR of ZEB1 to repress the translation of ZEB1. Interfering with the expression of ZEB1 significantly decreased the cell proliferation, migration activity, mRNA and protein expressions of α‐SMA, Col1A1 and Col3A. Furthermore, the expressions of miR‐590‐3p and ZEB1 were identified in infarct area of MI model in pigs. Collectively, miR‐590‐3p suppresses the cell proliferation, differentiation, migration and collagen synthesis of cardiac fibroblasts by targeting ZEB1. These works will provide useful biological information for future studies on potential roles of miR‐590‐3p as the therapeutic target to recover cardiac function following MI.

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Section: Discussionmentioning

confidence: 99%

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

Yuan

Pan

Wen

et al. 2019

J Cellular Molecular Medi

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“…Some of these miRNAs, including miR‐139, miR‐384, miR‐122, and miR‐188‐5p, have been shown to be downregulated in hepatocellular carcinoma and served as tumor suppressors. In contrast, some miRNAs are commonly upregulated and play oncogenic role in hepatocellular carcinoma, such as miR‐517a, miR‐151 and miR‐18a . However, in this research, we illustrated that miR‐490‐3p was low expressed in cell lines of Huh‐7, BEL‐7402 and HepG2.…”

Section: Discussionmentioning

confidence: 53%

“…Meanwhile, some research about miR‐490‐3p had been reported. For example, Ge and Gong showed that a tumor suppressor role of miR‐490‐3p in hepatocellular carcinoma via targeting transcriptional enhancer activator domain I ( TEAD I ) . Besides, Jiang et al illustrated that miR‐490‐3p upgrades proliferation and invasion in human HCCs via directly targeting TGF‐beta RII .…”

Section: Discussionmentioning

confidence: 99%

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MiR‐490‐3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma

Yu-xiang

Liu

2018

IUBMB Life

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The miR-490-3p was transfected into HepG2 cells to explore the correlation between miR-490-3p and hepatocellular carcinoma cell proliferation, apoptosis, and autophagy and its downstream target gene ATG7. Then we could possibly provide a mechanism for the treatment of hepatocellular carcinoma. MiR-490-3p was screened out by fold change > 4 and P < 0.01 using gene microarray data. The expression level of miR-490-3p was tested by qRT-PCR and the prognosis analysis was achieved by using TCGA data. The cell proliferation was tested via colony formation assay and CCK-8 after the miR-490-3p mimics were transfected into HepG2 cells; the variations of cell cycle and apoptosis was examined by flow cytometry assay; the number of autophagosome was observed by electron microscopy and the changes of autophagy-relative protein LC-II and LC-I as well as their ratio was tested by western blot. MiR-490-3p is low expressed in hepatocellular carcinoma cell lines and tissues. The results of TCGA showed that miR-490-3p high expression indicated better prognosis. After HepG2 cells were transfected with miR-490-3p mimics, cell viability was increased, cell proliferation was enhanced, cell cycle was blocked in G0/G1 phase, cell apoptosis rate was increased, the number of autophagosomes was reduced, autophagy-associated protein LC-II was decreased, and LC-I was increased and their ratio was decreased. After 3-MA was added, cell proliferation was declined, cell apoptosis rate was increased. Besides, the autophagy was inhibited by knocking down the ATG7, which promoted the cell apoptosis. MiR-490-3p could suppress cell proliferation, retard cell cycle and upgrade cell apoptosis by inhibiting autophagy in HCC cells via targeting ATG7. © 2018 IUBMB Life, 70(6):468-478, 2018.

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“…Several important genes were found to be the direct target of miR‐590‐3p in different disease models. For instance, in cancers, miR‐590‐3p suppresses hepatocellular carcinoma growth by targeting TEAD1 (Ge & Gong, ), inhibits epithelial–mesenchymal transition in intrahepatic cholangiocarcinoma by targeting SIP1 (Zu et al, ), and represses migration, invasion, and epithelial–mesenchymal transition in glioblastoma multiforme by targeting ZEB1 and ZEB2 (Pang, Zheng, Zhao, Xiu, & Wang, ). In myocarditis, miR‐590‐3p was also identified as a novel miRNA that could target nuclear factor κ‐B in vivo (Zhao et al, ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐590‐3p inhibits trophoblast‐dependent maternal spiral artery remodeling by repressing low‐density lipoprotein receptor‐related protein 6

Zhang

Pan

et al. 2018

Molec Gen & Gen Med

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BackgroundThe remodeling of maternal spiral artery following embryo implantation, which relies on well‐regulated trophoblast functions, is a pivotal process to ensure a successful pregnancy. Low‐density lipoprotein receptor‐related protein 6 (LRP6) and microRNAs (miRNAs, miRs) are suggested to be involved in angiogenesis and several vascular diseases; however, their functions in the control of trophoblast remain elusive. We therefore aimed to examine the roles of LRP6 and miR‐590‐3p in the regulation of trophoblast during the remodeling of maternal spiral artery.MethodsHTR‐8/SVneo cell, a trophoblast cell line, was utilized to study the effects of LRP6 and miR‐590‐3p on apoptosis, cell proliferation, migration, invasion, as well as tube formation. Expression of angiogenic factors placental growth factor (PlGF), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and activities of canonical Wnt/β‐catenin signaling pathway, which were implicated in the process of artery remodeling, were also examined.ResultsMiR‐590‐3p directly targeted 3′ untranslated region (3′‐UTR) of LRP6 mRNA and repressed LRP6 expression, which in turn inhibited proliferation, migration, invasion, as well as tube formation, and resulted in apoptosis in HTR‐8/SVneo cells. Further, inhibition of LRP6 through miR‐590‐3p significantly suppressed the expression of PlGF, MMPs, and VEGF and reduced the activation of Wnt/β‐catenin signaling pathway.ConclusionMicroRNAs‐590‐3p may inhibit trophoblast‐dependent maternal spiral artery remodeling, via both trophoblast invasion and endovascular formation, by repressing LRP6.

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MiR-590-3p suppresses hepatocellular carcinoma growth by targeting TEAD1

Cited by 45 publications

References 24 publications

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

MiR‐590‐3p regulates proliferation, migration and collagen synthesis of cardiac fibroblast by targeting ZEB1

MiR‐490‐3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma

MicroRNA‐590‐3p inhibits trophoblast‐dependent maternal spiral artery remodeling by repressing low‐density lipoprotein receptor‐related protein 6

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