MiR-574-5p mediates the cell cycle and apoptosis in thyroid cancer cells via Wnt/β-catenin signaling by repressing the expression of Quaking proteins

Abstract

Abstract. Thyroid cancer is the most frequently occurring type of endocrine tumor, with a rapidly increasing incidence rate. MicroRNA (miR)-574-5p is a candidate oncogene in various types of cancer. The present study identified that miR-574-5p affected the cell cycle distribution and apoptosis of BCPAP and FTC133 thyroid cancer cells via β-catenin/Wnt signaling by targeting Quaking proteins (QKIs). An MTT assay demonstrated that the knockdown of miR-574-5p suppressed the proliferation of the thyroid cancer cells. Fluorescence-activated cell sorting analysis demonstrated that the inhibition of miR-574-5p induced the G 1 /S phase arrest and apoptosis of the cells. Reverse transcription-quantitative polymerase chain reaction and western blot analyses revealed that the knockdown of miR-574-5p significantly upregulated the mRNA and protein expression levels of QKIs. Furthermore, western blot analysis identified that the knockdown of miR-574-5p also repressed the Wnt/β-catenin pathway via downregulating the expression of β-catenin, cyclin D1 and survivin, and upregulating the phosphorylation of β-catenin. The further depletion of QKIs in combination with the knockdown of miR-574-5p not only increased the expression of β-catenin, cyclin D1 and survivin, but also rescued the apoptosis of thyroid cancer cells induced by the miR-574-5p knockdown. In conclusion, these findings indicated that the aberrant upregulation of miR-574-5p may be oncogenic, through regulating the Wnt/β-catenin pathway by targeting QKIs.

miR‑25‑3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3

“…Cell transfection. U251 and T98G cells in the logarithmic phase were seeded at a density of 5x10 5 termed miR-25 in + siFBXW7) or 100 nM miR-25 inhibitor and 100 nM DKK3 siRNA (cat. no.…”

“…MicroRNAs (miRs), which are small endogenous noncoding RNAs comprising 22-25 nucleotides, regulate gene expression at the transcriptional and/or post-transcriptional level by binding to the 3'untranslated region (UTR) of their target mRNA (5,6). By repressing protein translation or promoting mRNA degradation, miRs play important roles in multiple cellular processes, including cell proliferation, survival, apoptosis, differentiation and motility (6)(7)(8).…”

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

“…MiR-574-5p are implicated in the pathophysiology of many tumors, including breast cancer, colorectal cancer and other oncoma [ 4 , 5 , 6 ]. In addition, miR-574-5p is a very important regulator for cell migration and proliferation [ 7 , 8 , 9 ]. Based on our laboratory sequencing data, miR-574-5p is differentially expressed in the colostrum and peak lactation periods [ 10 ].…”

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

“…miR-574-5p is one of the most well-studied miRNAs that is linked to thyroid carcinomas, FTC and PTC in particular, via Wnt pathway. Up-regulation of miR-574-5p was reported by different groups to promote proliferation, Epithelial-Mesenchymal Transition (EMT), invasion, migration and inhibit apoptosis (63)(64)(65). miR-574-5p exerts its oncogenic effects via targeting Forkhead Box N3 (FOXN3), Quaking protein 5-7 (QKI5-7), and Suppressor of Cancer Cell Invasion (SCAI) transcripts.…”

“…Collectively, this results in the activation of the Wnt signalling pathway. This, in turn, up-regulates β-catenin, N-cadherin (a mesenchymal biomarker), Snail, c-Myc, cyclin D1 and survivin proteins (63)(64)(65), leading to downstream oncogenic phenotypes and thyroid cancer development.…”